clear plates. Avoid bubbles.
4. Read at 490 nm excitation and 520 nm emission, or
select the filter pairing which most closely
approximates this range.
5. Fluorescein has an optimal excitation
clear plates. Avoid bubbles.
4. Read at 490 nm excitation and 520 nm emission, or
select the filter pairing which most closely
approximates this range.
5. Fluorescein has an optimal excitation
.M. and Pagano, M., Regulation of the G1 to S
transition by the ubiquitin pathway. FEBS Lett., 490, 179-
189 (2001).
2. Yew, P.R., Ubiquitin-mediated proteolysis of vertebrate
G1 and S-phase regulators
sequence 5’-CTATCATGCCATACCGCGAAAGG-3’,
available from Sigma-Genosys, is recommended for
sequencing through the N-terminal junction.
pT7-FLAG-2 Features
Feature Map Position
T7 Promoter 72-91
lacO 92
L.M. and Pagano, M., Regulation of the
G1 to S transition by the ubiquitin pathway. FEBS
Lett., 490, 179-189 (2001).
2. Yew, P.R., Ubiquitin-mediated proteolysis of
vertebrate G1 and S-phase regulators
the Office of Technology
Commercialization and Partnerships, Brookhaven National
Laboratory, Bldg. 490-C, P.O. Box 5000, Upton, New York
11973-5000, telephone (631)-344-7134.
2. No materials that contain
the Office of Technology Commercialization and Partnerships,
Brookhaven National Laboratory, Bldg. 490-C, P.O. Box
5000, Upton, New York 11973-5000, telephone (631)-344-
7134.
2. No materials that contain
the Office of Technology Commercialization and Partnerships,
Brookhaven National Laboratory, Bldg. 490-C, P.O. Box
5000, Upton, New York 11973-5000, telephone (631)-344-
7134.
2. No materials that contain
the Office of Technology Commercialization and Partnerships,
Brookhaven National Laboratory, Bldg. 490-C, P.O. Box
5000, Upton, New York 11973-5000, telephone (631)-344-
7134.
2. No materials that contain
the Office of Technology
Commercialization and Partnerships, Brookhaven National
Laboratory, Bldg. 490-C, P.O. Box 5000, Upton, New York
11973-5000, telephone (631)-344-7134.
2. No materials that contain
the Office of Technology Commercialization and Partnerships,
Brookhaven National Laboratory, Bldg. 490-C, P.O. Box
5000, Upton, New York 11973-5000, telephone (631)-344-
7134.
2. No materials that contain
above and
add to 490 µL of Assay Buffer. This 1000 pg/mL standard concentration will be
Standard 7 in the table below.
3. Label 6 polypropylene microfuge tubes as Std 6, Std 5, Std 4
Plate
A
vg
. M
FI
Recovery of Total Events (%)
With 2 Hr. Incubation, 5 washes, 8"Hg, n =32
Single Events 87-91%
0%
20%
40%
60%
80%
100%
0.45 µm HV
MultiScreen Filter Plate
1.2 µm
and the Propidium Iodide (PI) counterstain can be
viewed with a “long pass” filter for FITC (ex. 490 nm & em. 520 nm);
this filter allows sufficient PI signal to “bleed through”. A “dual pass”
filter