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药物研发2.0_药物发现_药物代谢_药物分析-默克生命科学
默克致力于开发创新型产品，从专业角度分析制药行业发展，挖掘客户需求。从基础科学研究到审批生产，默克完整、高效的解决方案，能够帮助新药研发的科学家们，加快药物研发速度，解决药物研发中的难题。
一种简单的超滤法蛋白富集
人血浆蛋白质组是生物标志物发现和研究的广阔资源。
用于梯度离心的Ficoll® 400
Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。
胰蛋白酶抑制剂
天然胰蛋白酶抑制剂也称为丝氨酸蛋白酶抑制剂（serpins），是最大和最多样化的蛋白酶抑制剂家族。Serpins通过抑制体内丝氨酸蛋白酶来控制蛋白质的活化和分解代谢。
PCR/qPCR数据分析
传统的PCR完成后，会通过琼脂糖凝胶——最近更常使用的是毛细管电泳系统——来分辨并分析数据。
推荐单核细胞标准分离方法
以下操作步骤已接受Ficoll-Paque PLUS评估，推荐用于分离正常血样以尽可能提高可重现性。
PCR/qPCR/dPCR实验设计
整个PCR工作流程易受引入变量的影响。许多变量无法避免，例如样本来源和逆转录步骤的要求。实验设计也波动较大，可能决定PCR的成败，影响实验的可重复性和灵敏度。
胃肠道癌症中的免疫组织化学应用
结直肠癌是男性和女性中第三大常见癌症。预计2016年将发生136,000例结直肠癌。
FITC标记多糖
包括FITC-葡聚糖、FITC-DEAE-葡聚糖(FDD)、FITC-羧甲基 - 葡聚糖（FCM-葡聚糖）在内的FITC标记多糖的补充信息。本页面主要介绍了重均分子量对于对应分子尺寸和其他物理性质的影响
Extractables Study with LC-UV/MS
Reliable identification and quantification of unknown extractables of a single-use system (SUS) with a 21 component CRM mix for liquid chromatography (LC-UV/MS) methods using Ascentis® C18 column.
Column Packing and Preparation for Affinity Chromatography of Antibodies
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Optimizing Crude Sample Plant PCR
Overcoming potent inhibitors and sample diversity in direct plant PCR.
Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins
This page describes efficient column packing and preparation for affinity chromatography of GST-tagged proteins using Cytiva products.
Pressure-Temperature Calculator for Solvents
Use the calculator to determine the boiling point or pressure of solvents based on the Antoine Equation and see solvent attributes. View a list of products based on the solvent CAS number.
Extraction and Analysis of Lasix (Furosemide) in Horse Serum
Studies have suggested that there is a correlation between furosemide and improved racing performance, prompting the horse racing industry to monitor the use of this drug
Sample Preparation in Ion Exchange Chromatography
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
General Adherent Cells
Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.
NHC-based Palladium Catalysts
In collaboration with Umicore AG and Co.,1 we are pleased to offer a series of robust Pd(II) and Pd(0) complexes employed as the linchpin in C–C bond forming reactions.
Simplified LC-MS/MS Method for Glyphosate, AMPA, and Glufosinate in Oat-Based Cereals
A simplified LC-MS/MS method to determine glyphosate and related compounds using a Supel™ Carbon LC U/HPLC column for stability under higher pH conditions and sufficient retention for the polar analytes in the presence of methanol as an extraction solvent.
Amicon® Pro Purification System – Protein Purification
Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.
hESC Transduction Protocol
A detailed protocol for the transduction of human embryonic stem cells using Lentiviral Vectors. Includes product list and references.
Induced Pluripotent Stem Cell Reprogramming Protocols
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
Validation of shorter protocol for detection of Salmonella enterica subsp. Enterica in peanut butter samples followed by a rRNA detection system
Food-borne pathogens Salmonella is commonly evaluated in manufacturing of peanut butter and other food products.
HPLC Separation of Ginsenosides from American Ginseng
The HPLC method for ginsenoside separation was optimized using a ginsenoside standard mixture and then applied to a sample of American Ginseng root. The experimental conditions and chromatograms are shown.
ADCC Protocol using BioIVT Cryopreserved NK Effector Cells
Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.
Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column
Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column
Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications
Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
Myokine Analysis | MILLIPLEX® Multiplex Assays
Myokine analysis with multiplex immunoassays, such as the MILLIPLEX® Human Myokine Panel, allow researchers to simultaneously measure multiple muscle-secreted factors, providing more insights into related diseases.
Organosilanols
Over the past several years, the Pd-catalyzed cross-coupling of silicon compounds (Hiyama coupling) has rapidly gained acceptance as a suitable alternative to more commonly used methods such as Stille (Sn), Kumada (Mg), Suzuki (B), and Negishi cross-couplings (Zn).
ATRP Ligands and Initiators for the Clean Synthesis of Functional Polymers
Atom transfer radical polymerization (ATRP) has emerged as one of the most successful synthetic techniques for the preparation of polymers with predetermined molecular weights, narrow molecular weight distributions, and high degrees of chain end functionalities.
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