and 179° 175 - 176°
Specific rotation, [α]D
25
10 mg/mL solution in dioxane 〈781〉
between +76° and +83° + 77°
Water Method 1 〈921〉 not more than 3.5% 3.3%
Chromatographic purity 〈621〉 not
and 179° 175 − 178°
Specific rotation, [α]
D
25
10 mg/mL solution in
dioxane 〈781S〉
between +76° and +83° + 80°
Water Method 1 〈921〉 not more than 3.5% 3.0
Chromatographic purity 〈621〉 not more
179° 175° − 178°
Specific rotation, [α]
D
25
10 mg/mL solution in
dioxane 〈781S〉
between +76° and +83° + 80°
Water Method 1 〈921〉 not more than 3.5% 3.0
Chromatographic purity 〈621〉 not more
0.1
0
A
b
s
o
r
b
a
n
c
e
Bio-Tek Uvikon XL
Capillary Gas Chromatography
Column Supelco SPB-1 (Cat. No. 2-4162)
I.D. 0.20 mm Length 15 m Film Thickness 0.20 µm
Oven Temperature 50°C (2
Percentage of Improvement
Decreased solvent usage by 76%
Reduced quantity of chemical usage
tBuXPhos (638080)
N/A
N/A
N/A
N/A
0= Most Desirable
92%
Reduced chemical processing
adsorption and subse-
quent elution.
References
1. Broze, G. J., Jr., et al., J. Clin. Invest., 76, 937
(1985).
2. Broze, G. J., Jr., and Majerus, P. W., J. Biol. Chem.,
255, 1242 (1980).
al., Methods in Cell Biology, 30,
449 (1989).
5. Wulf, E. et al., Proc. Nat. Acad. of Sci., USA, 76,
4498 (1979).
6. Faulstich, H. et al., Exp. Cell Res., 144, 73 (1983).
7. Lawson, M.A., and Maxfield
Netherlands
t 31 20 655 79 28
f 31 20 659 76 73
United Kingdom
t 0800 376 8660
f 0800 279 1117
All Other European
Countries
t 31 (0) 20 659 60 51
f 31 (0) 20 659 76 73
L AT I N AME
to spin to 50 µL retentate at 3000 x g, 25 °C
Initial Volume of Time to Spin to
0.5 mg/mL 50 µL Retentate
Cytochrome c (µL) (minutes)
100 µL 15
200 µL 25
requirement
Specific rotation
10 mg per mL in dioxane 〈781 S〉
between +72° and +80° +76°
Loss on drying 〈731〉 not more than 0.5% meets requirement
Residue on
meets requirement
Specific rotation
10 mg per mL in dioxane 〈781 S〉
between +72° and +80° +76°
Loss on drying 〈731〉 not more than 0.5% meets requirement
Residue on ignition 〈281〉 not more