# 70-501 Page 3 of 15
Tech Support 1-800-548-7853
www.upstate.com
III. UNIVERSAL TFA KIT COMPONENTS
A. Provided Kit Components (Note Storage Temperatures)
Store
Connect the incubator to a standard grounded outlet:
■ 115V 50/60 Hz for XX63 501 15
■ 230V 50/60 Hz for XX63 502 30
2. Turn on the power using the power switch. The white
caspase 8
mediates the mitochondrial damage in the Fas
pathway of apoptosis. Cell, 94, 491-501 (1998).
4. Zha, J. et al., Posttranslational N-myristoylation of
BID as a molecular switch for targeting
alterations. Transplantation, 76(3), 501-508
(2003).
8. Kim, J. S., and Southard, J. H., Phospholipid
metabolism of hypothermically stored rat
hepatocytes. Hepatology, 30(5), 1232-1240
(1999).
9.
low speed) to gently agitate the nuclear suspension at 4°C for 30-60 minutes.
4. Centrifuge the nuclear suspension at 16,000 x g for 5 minutes at 4°C.
5. Transfer the supernatant to a fresh tube. This
the total recovered volume on 4%
agarose gel containing ethidium bro-
mide. The DNA marker is an MspI digest
of puc18 (mw- 67, 89, 110, 147, 190,
242, 353, 404, 49, and 501 bases). Yeast
tRNA was loaded
the total recovered volume on 4%
agarose gel containing ethidium bro-
mide. The DNA marker is an MspI digest
of puc18 (mw- 67, 89, 110, 147, 190,
242, 353, 404, 49, and 501 bases). Yeast
tRNA was loaded
, C. L., The
phosphatidylinositol 3-kinase Akt pathway in human
cancer. Nat. Rev. Cancer, 2, 489-501 (2002).
AH,PHC 05/05-1
Sigma brand products are sold
µl sample
size).
4. The appropriate range of this assay is 0.078 ug/mL to 5.0 ug/mL Human APO
AIV (20 µl sample size). Any result greater than 5.0 ug/mL in a 20 µL of 1/501
diluted sample
protein sample (section A, step 4) by
adding 25 µl of the 200 mM Tributylphosphine
Solution per 1 ml of protein solution. Incubate the
sample at room temperature for 15-30 minutes.
2. Alkylate
bisacrysmide) minigel (1.5 mm
width) at 30 mA/gel ~1-1.5 hours.
3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)
4. Stain the transferred bands with Chemicon
human PDGF Rα, a 501 amino acid residue,
has a predicted molecular mass of approximately 56
kDa. PDGF Rα and PDGF Rβ share 44 % sequence
identity. Within the extracellular domain, 30 % of the
amino acid
Sawyers, C.L., The
phosphatidylinositol 3-Akt pathway in human
cancer. Nat. Rev. Cancer, 2, 489-501 (2002).
ADM,SH,PHC,MAM 06/11-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
IB to every 1 gram of purified
wet inclusion body pellet.
4. Vortex this suspension for 1 minute.
5. Shake the suspension for 30 minutes at room
temperature.
6. Centrifuge the suspension at
immunoglobulins, which do not
specifically bind to the peptide. Recombinant human
PDGF sRα is a 501 amino acid (~56 kDa)
transmembrane protein, expressed in a mouse
myeloma NSO cell line.1.
Anti-Platelet-Derived