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Robert W Hepler et al.
Protein science : a publication of the Protein Society, 27(11), 1923-1941 (2018-08-26)
Chlamydial major outer membrane protein (MOMP) is the major protein constituent of the bacterial pathogen Chlamydia trachomatis. Chlamydia trachomatis Serovars D-K are the leading cause of genital tract infections which can lead to infertility or ectopic pregnancies. A vaccine against
Kazuhiro Sakamaki et al.
Biochemistry and biophysics reports, 3, 175-189 (2015-08-07)
Cellular FLICE-like inhibitory protein (c-FLIP, gene symbol CFLAR) was first identified as a negative regulator of death receptor-mediated apoptosis in mammals. To understand the ubiquity and diversity of the c-FLIP protein subfamily during evolution, c-FLIP orthologs were identified from a
Masahiko Ajiro et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 24(18), 4518-4528 (2018-05-02)
Purpose: Cervical cancer is one of the leading causes of cancer-related deaths among women worldwide. The purpose of this study is to assess the therapeutic effect of the newly developed cyclin-dependent kinase 9 (CDK9) inhibitor FIT-039 on cervical neoplasia induced
Diego J M Gómez-Coronado et al.
Journal of agricultural and food chemistry, 51(18), 5196-5201 (2003-08-21)
Extraction and chromatographic conditions for measuring tocopherols from Laurus nobilis were optimized. Newly harvested leaves were dried in a microwave oven and crushed; then, alpha- and gamma-tocopherol and tocol, added as internal standard, were directly extracted from portions of ground
F J Rupérez et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 800(1-2), 225-230 (2003-12-31)
An HPLC method for Vitamins A and E in rat plasma has been developed. The main goals of the method are the small amount of sample, 50 microl, and the direct extraction of analytes in one step with acetone, which
R Nageswara Rao et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 873(1), 119-123 (2008-08-30)
A highly sensitive and specific liquid chromatography/tandem mass spectrometric (LC-MS/MS) method for investigating the pharmacokinetics of adrafinil in rats was developed. Rat serum pretreated by solid-phase extraction (SPE) was analyzed by LC-MS/MS with an electrospray ionization (ESI) interface. The mobile
A E Leonard et al.
The Biochemical journal, 350 Pt 3, 765-770 (2000-09-06)
The Saccharomyces cerevisiae protein ELO2p is involved in the elongation of saturated and monounsaturated fatty acids. Among several sequences with limited identity with the S. cerevisiae ELO2 gene, a consensus cDNA sequence was identified from the LifeSeq(R) database of Incyte
György Székely et al.
Drug testing and analysis, 6(9), 898-908 (2013-11-19)
This paper discusses a design of experiments (DoE) assisted optimization and robustness testing of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method development for the trace analysis of the potentially genotoxic 1,3-diisopropylurea (IPU) impurity in mometasone furoate glucocorticosteroid. Compared to the
Jiaxin Pi et al.
Pakistan journal of pharmaceutical sciences, 28(1), 213-220 (2015-01-03)
In targeting delivery system research on salvianolic acid B, it's vital but hard to evaluate the tissue distribution for its low concentrations in tissues. So the simple, rapid, selective and sensitive UPLC-MS/MS method was provided hereby to determine the concentration
Chuansong Zhan et al.
Biochemical and biophysical research communications, 495(1), 1271-1277 (2017-11-29)
Anemone flaccida Fr. Shmidt (Ranunculaceae), known as 'Di Wu' in China, is a perennial herb which has long been used to treat arthritis. The rhizome of A. flaccida contains pharmacologically active components i.e. oleanane-type triterpenoid saponins. Oleanolic acid is natural triterpenoid
S Bhoopathy et al.
Journal of pharmaceutical and biomedical analysis, 37(4), 739-749 (2005-03-31)
A direct injection LC/MS/MS method involving a novel incubation technique was developed for the inhibition screening of CYP 2D6 and CYP 3A4 isoenzymes using dextromethorphan and midazolam as probe substrates. Both assays were performed using an electrospray ionization source in
R Nageswara Rao et al.
Journal of pharmaceutical and biomedical analysis, 53(4), 833-842 (2010-07-14)
Forced degradation of ritonavir (RTV), under the conditions of hydrolysis (acidic, basic and neutral), oxidation, photolysis and thermal stress as prescribed by ICH was studied using LC-MS/MS. Eight degradation products were formed and their separation was accomplished on Waters XTerra
E Dijkman et al.
Journal of chromatography. A, 926(1), 113-125 (2001-09-14)
This study investigated the effects of matrix interferences on the analytical performance of a triple quadrupole mass spectrometric (MS-MS) detector coupled to various reversed-phase liquid chromatographic (LC) modes for the on-line determination of various types of acidic herbicides in water
Ghada M Hadad et al.
Talanta, 79(5), 1360-1367 (2009-07-29)
A stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) method has been developed which can separate and accurately quantitate paracetamol, dantrolene, cetirizine and pseudoephedrine. The method was successfully validated for the purpose of conducting stability studies of the four analytes in quality
R D Yeole et al.
Journal of chromatography. A, 1108(1), 38-42 (2006-01-24)
A sensitive, simple, specific, precise, accurate and rugged method for the assay and determination of enantiomeric purity of S-(-)-9-fluoro-6,7-dihydro-8-(4-hydroxypiperidin-1-yl)-5-methyl-1-oxo-1H,5H-benzo[i,j]quinolizine-2-carboxylic acid L-arginine salt tetrahydrate (WCK 771) in bulk drug has been developed. The method is RP-HPLC using endcapped C-18 stationary phase
Raja Reddy Kallem et al.
Biomedical chromatography : BMC, 20(12), 1289-1294 (2006-09-29)
For pharmacokinetic and toxicokinetic purpose a simple HPLC-UV method has been developed and validated for the estimation of DRF-4848, a novel COX-2 inhibitor in rat plasma. A liquid-liquid extraction was used to extract DRF-4848 and internal standard (IS, DRF-4367) from
Haijun Yang et al.
Journal of pharmaceutical and biomedical analysis, 49(1), 140-144 (2008-11-28)
Annonaceous acetogenins (ACGs) isolated from Annonaceae plants exhibited a broad range of biological bioactivities such as cytotoxic, antitumoral, antiparasitic, pesticidal and immunosuppresive activities. However, their structures were liable to change at more than 60 degrees C and their extraction yields
Andrew N Hobbs et al.
Drug discovery today, 18(3-4), 148-154 (2012-09-25)
Practical purifications of hydrophilic fragments and lead/drug-like molecules are reviewed, focussing particularly on the rational use of C-18 reverse phase flash chromatography by exploiting the relationship between retention time and distribution coefficients. Logical process changes have been implemented to suit
Blessings Thuboy et al.
Biomedical chromatography : BMC, e4269-e4269 (2018-05-05)
A bioanalytical method was developed and validated for the quantification of capreomycin (Cm) analogs, Cm IA and Cm IB, in human plasma. This implemented ion-pairing solid phase extraction, followed by ion-pairing high-performance liquid chromatography, with tandem mass spectrometry detection. Chromatographic
Sheelendra Pratap Singh et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 876(1), 1-7 (2008-11-14)
A highly sensitive and specific HPLC-ESI-MS/MS method has been developed and validated for the estimation of centchroman with 100 microL rat plasma using tamoxifen as an internal standard (IS). The assay procedure involved a single-step, liquid-liquid extraction of centchroman and
Roman Kand'ár et al.
Journal of pharmaceutical and biomedical analysis, 43(4), 1382-1387 (2006-12-22)
A HPLC method for determination of both reduced (GSH) and oxidized (GSSG) glutathione in plasma, whole blood and rat hepatocytes has been developed and evaluated. Reduced glutathione reacts with orthophthaldehyde (OPA) to form a stable, highly fluorescent tricyclic derivate at
Antal Péter et al.
Journal of chromatography. A, 1031(1-2), 171-178 (2004-04-03)
Direct and indirect reversed-phase (RP) high-performance liquid chromatographic methods were developed for the separation of enantiomers of 18 unnatural beta-amino acids, including several beta-3-homo amino acids. The direct separations of the underivatized analytes were performed on chiral stationary phases (CSPs)
Ming Huang et al.
Cancer research, 78(18), 5431-5445 (2018-08-04)
Small GTPases of the Ras superfamily are master regulators of intracellular trafficking and constitute essential signaling components in all eukaryotes. Aberrant small GTPase signaling is associated with a wide spectrum of human diseases, including cancer. Here, we developed a high-throughput
Hui Chen et al.
Talanta, 107, 88-94 (2013-04-20)
A method to study Abl1 tyrosine kinase inhibitors (TKIs) by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) was developed and validated. Chromatographic separation was achieved on a Symmetry(®) C-18 column using a gradient. The detection was performed by selected ion monitoring (SIM)
Dan Song et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 33(17), 2133-2135 (2008-12-11)
To determine lobetyolin in the root of Codonopsis tangshen from the various cultivation areas. A Supelco Discovery C18 Column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-0.5% acetic acid in water (20:80) as the mobile phase and
R Nageswara Rao et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 94-103 (2007-10-24)
A reversed-phase high performance liquid chromatographic (RP-HPLC) method for evaluation of purity of tamsulosin in bulk drugs and pharmaceuticals was developed. The separation was accomplished on an Inertsil C(18) column using 10 mM ammonium acetate: acetonitrile as a mobile phase
P Shalem Raj et al.
International journal of systematic and evolutionary microbiology, 62(Pt 5), 1069-1074 (2011-06-28)
Two strains (JA492(T) and JA590) of spiral-shaped, anaerobic, Gram-stain-negative, motile, purple non-sulfur bacteria were isolated from aquatic sediments from a bird sanctuary and a stream, respectively, and were characterized by a polyphasic taxonomic approach. Bacteriochlorophyll a and carotenoids (rhodopin, lycopene
Yulia Y Tyurina et al.
Free radical biology & medicine, 44(3), 299-314 (2008-01-25)
Although gamma-irradiation-induced tissue injury has been associated with lipid peroxidation, the individual phospholipid molecular targets have not been identified. We employed oxidative lipidomics to qualitatively and quantitatively characterize phospholipid peroxidation in a radiosensitive tissue, the small intestine, of mice exposed
Rao N V S Mamidi et al.
Biomedical chromatography : BMC, 17(6), 417-420 (2003-09-19)
To the best of our knowledge, bioanalytical methods to determine rosiglitazone in human plasma reported in literature use internal standards that are not commercially available. Our purpose was to develop a simple method for the determination of rosiglitazone in plasma
Katakam Prakash et al.
Scientia pharmaceutica, 82(3), 571-583 (2015-04-09)
A selective and sensitive liquid chromatography-tandem mass spectrometric method (LC-MS/MS) has been developed and validated for the quantification of zileuton in human plasma. Deuterated internal standard (zileuton D4) was used as the internal standard (ISTD). Zileuton was extracted by liquid-liquid
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