flammability and toxicity hazards
53%
100
Re-engineered Score
Previous Score
51 35
30%
43% Increased yield. Used less raw materials
43%
N/A
N/A
0
The life science business
46 h at 33-35 °C 70-200 %
Escherichia coli 8739 10-100 42-46 h at 33-35 °C 70-200 %
Pseudomonas aeruginosa
27853 10-100 42-46 h at 33-35 °C 70-200 %
9027 10-100 42-46 h at
146444); digits 4-9: lot number; digits 10-14: batch
specific individual number; digits 15-20: expiration date (YY/MM/DD). The agar is aerobically incubated
for 24-48 hours at 30-35 °C. For testing
aureus
6538 10-100 20-24 h at 35-37 °C
70-200 %; good growth;
yellow, medium-sized
colonies with slight double
hemolysis
Escherichia coli 8739 10-100 20-24 h at 35-37 °C
70-200 %; good growth
Results
Escherichia coli 8739 10-100 20-24 h at 33-35 °C good growth; pronounced
turbidity
Pseudomonas
aeruginosa
9027 10-100 20-24 h at 33-35 °C good growth; pronounced
turbidity
Staphylococcus
Germany and/or its affiliates. All Rights Reserved.
TN1242EN00 Page 4 of 4
Merck KGaA
64271 Darmstadt, Germany
Fax: +49 (0) 61 51 / 72-60 80
mibio@merckgroup.com
Find contact information for your
Germany and/or its affiliates. All Rights Reserved.
TN5556EN00 Page 4 of 4
Merck KGaA
64271 Darmstadt, Germany
Fax: +49 (0) 61 51 / 72-60 80
mibio@merckgroup.com
Find contact information for your
Incubation Expected Results
Escherichia coli 25922 ≥ 104 24 h at 35 °C Growth inhibited
Salmonella typhimurium 14028 10-100 24 h at 35 °C
Growth, enrichment from
a mixed inoculum
Please refer to
Incubate at 35 °C for 1.5 to 2.0 hours in a humid atmosphere.
3. Transfer the filter - again top side up - to the surface of the agar. Avoid entrapment of air.
4. Incubate inverted plates at 35 °C
Results
Escherichia coli 25922 About 99 % 24 h at 35 °C
Enrichment from a mixed
inoculum ≤ 10 %
Salmonella typhimurium 14028 About 1 % 24 h at 35 °C
Enrichment from a mixed
inoculum
Candida albicans 10231 10-100
3 d at 30-35 °C Good growth, sediment
4 d at 20-25 °C Good growth, sediment
Candida glabrata 70614 (DSM) 10-100 4 d at 20-25 °C Good growth, sediment
Microsporum
Preparation
Suspend 51 g in 1 litre of distilled or deionized water containing
20 ml of ethanol 96 % and heat to boiling to dissolve completely.
Do not autoclave! Cool to 45-50 °C. Dispense 4 ml amounts into
25416 10-100 24-28 h at 33-35 °C 70-200 %
Ralstonia pickettii 27511 10-100 24-28 h at 33-35 °C 70-200 %
Methylobacterium
extorquens
15911
(NBRC #)
50-200 4-7 d at 20-25
6538 10-100 20-24 h at 30-35 °C 50-200
Clostridium sporogenes 11437 10-100 44-48 h at 30-35°C, anaerobicly 50-200
Pseudomonas aeruginosa 9027 10-100 20-24 h at 30-35 °C 50-200
Bacillus subtilis