Version: 07
Content Version: November 2020
Photometric endpoint determination.
Cat. No. 11 746 081 001 1 test combination
approximately 64 assays
Store the product at +2 to +8°C.
https
and splenocytes from BALB/c
mice immunized with a synthetic peptide corresponding
to amino acids 540-565 of human RNF20. The isotype is
determined by a double diffusion immunoassay using
Mouse Monoclonal
, J. Cell Biol., 185: 27-34 (2009).
5. Liang, X.T., et al., J. Gastroenterol. Hepatol., 26:
544-540 (2001).
Notice
We provide information and advice to our customers
on application technologies
dilutions by titration.
References
1. Simons, M., and Trotter, J., Curr. Opin. Neurobiol.,
17, 533-540 (2007).
2. Quarles, R. H., Cell. Mol. Life Sci., 59, 1851-1871
(2002).
3. Snipes, G. J., et al.
completely dissolved,
the optical density of each well was measured in a
microplate reader set at 540 nm. For background noise
subtraction, the second wavelength was set at 690 nm.
The Neutralization
stained and scored for cytopathic
effect by measurement of optical densities in a
microplate reader at 540 nm.
The Neutralization Dose50 (ND50) for this antibody is
defined as that concentration of antibody
stained and scored for cytopathic
effect by measurement of optical densities in a
microplate reader at 540 nm.
The Neutralization Dose50 (ND50) for this antibody is
defined as that concentration of antibody
months at +2 to +8°C
�-Gal ELISA y Version 07
http://www.sigmaaldrich.com/site-level/corporate/worldwide-offices
3.1 Preparation of working solutions, continued
8 sigma-aldrich.com
to bring all salts into solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store the unused portion
at 2-8°C for up to one month.
D. Preparation of Serum Matrix
to bring all salts into solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store unused portion
at 2-8C for up to one month.
D. Preparation of Serum Matrix
to bring all salts into
solution. Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store the
unused portion at 2-8°C for up to one month.
C. Preparation of lyophilized HeLa Cell
to bring all salts into solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store unused portion
at 2-8°C for up to one month.
D. Preparation of Mouse MMP Standard
to bring all salts into solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store the unused
portion at 2-8°C for up to one month.
D. Preparation of Human Kidney Injury
to bring all salts into
solution. Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store
unused portion at 2-8C for up to one month.
Standard 6 Standard 5 Standard 4
to bring all salts into solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store the unused
portion at 2-8°C for up to one month.
D. Preparation of Canine Kidney Toxicity
to bring all salts into solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store the unused
portion at 2-8°C for up to one month.
D. Preparation of Serum Matrix
solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store unused portion
at 2-8°C for up to one month.
MADPNMAG-70K-01 Rev. 07-JUN-2017 PAGE 10
solution. Dilute
60 mL of 10X Wash Buffer with 540 mL deionized water. Store unused portion at 2-8°C for
up to one month.
MADCYMAG-72K Rev. 07-JUN-2017 PAGE 9
mix to bring all salts into solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store the unused
portion at 2-8°C for up to one month.
D. Preparation of Serum Matrix
This step is