quantitated by the diaphorase mediated reduction of
resazurin to the fluorescent dye resorufin
(ex = 540 nm/em = 590 nm).
Components
The kit is sufficient for 200 fluorometric reactions
D2HG Standard
Tox-4 Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine
Tox-4 Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine
Tox-4 Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine
Tox-4 Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine
Tox-4 Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine
Tox-4 Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine
Tox-4 Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine
to 450 nm.
If wavelength correction is available, set to 540 nm
or 570 nm. If wavelength correction is not
available, subtract readings at 540 nm or 570 nm
from the readings at 450 nm. This subtraction
room temperature and mix to bring all salts into solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store unused portion
at 2-8C for up to one month.
D.
room temperature and mix to bring all salts into solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store unused portion
at 2-8C for up to one month.
D.