Methods, 184(1), 39-51 (1995).
7. Homberg, C.H., et al., Human neutorphils lose their
surface Fc gamma RIII and acquire Annexin V
binding sites during apoptosis in vitro. Blood,
85(2), 532-540 (1995).
Methods, 184(1), 39-51 (1995).
7. Homberg, C.H., et al., Human neutorphils lose their
surface Fc gamma RIII and acquire Annexin V
binding sites during apoptosis in vitro. Blood,
85(2), 532-540 (1995).
Methods, 184(1), 39-51 (1995).
7. Homberg, C.H., et al., Human neutorphils lose their
surface Fc gamma RIII and acquire Annexin V
binding sites during apoptosis in vitro. Blood,
85(2), 532-540 (1995).
550 nm (540-560 nm) excitation and long pass >610 nm emission/barrier filter pairing.
Lysosomes will appear red. When illuminating with a blue light (480 nm) excitation filter, a green light (540-550
stain is subsequently dissolved in 100 L of 33% acetic
acid and the optical density is read at 540 nm.
The Neutralization Dose50 (ND50) is 0.5-2 g/ml in the
presence of 0.1 g/ml of soluble
terminus of aminoacyl-tRNA and competes for
binding to the A-site of the large ribosomal subunit.
540��� �5 mg
100 mg
€47
€157
Selection Agents
* Size available only in Europe.
For technical
five main points remain unchanged:
1. Read the absorbance 562 nm. For a multiwell plate
reader, which does not have the exact filter, a filter
in the range of 540–590 nm can be substituted
(See Graph
4. Add 540 µL of 37% formaldehyde (or 1100 µL of 18.5% formaldehyde) directly to 20 mL of
growth media to crosslink. Gently swirl dish to mix.
� Final concentration of formaldehyde is 1%. Use high
4. Add 540 µL of 37% formaldehyde (or 1100 µL of 18.5% formaldehyde) directly to 20 mL of
growth media to crosslink. Gently swirl dish to mix.
� Final concentration of formaldehyde is 1%. Use high
room temperature and mix to bring all salts into solution.
Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store the unused portion
at 2-8°C for up to one month.
room temperature and mix to bring all salts into
solution. Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store
unused portions at 2-8C for up to one month.
D. Preparation
room temperature and mix to bring all salts into
solution. Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store unused
portions at 2-8 °C for up to one month.
Preparation
of 10X Wash Buffer with 540 mL deionized water. Store unused
portion at 2-8 °C for up to one month.
Preparation of Human Neurodegenerative Disease Panel 4 Standard
1. Prior to use, reconstitute
4. Add 540 µL of 37% formaldehyde (or 1100 µL of 18.5% formaldehyde) directly to 20 mL of
growth media to crosslink. Gently swirl dish to mix.
� Final concentration of formaldehyde is 1%. Use high