c-KIT (544-end) Active (C0624) - Datasheet
c-KIT (544-end), Active, GST-tagged, human
Precisio Kinase
recombinant, expressed in Sf9 cells
Catalog Number C0624
Lot Number 109K0817
Storage
150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA,
0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼82 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 544–736 nmole/min/
c-KIT (544-end) Active (C0624) - Datasheet
c-KIT (544-end), active, GST-tagged, human
PRECISIO Kinase
recombinant, expressed in Sf9 cells
Catalog Number C0624
Lot Number 110M0842
Storage
c-KIT (544-end) Active (C0624) - Datasheet
c-KIT (544-end), active, GST-tagged, human
PRECISIO
Kinase
recombinant, expressed in Sf9 cells
Catalog Number C0624
Storage Temperature
c-KIT (544-end) Active (C0624) - Datasheet
c-KIT (544-end), active, GST-tagged, human
Precisio Kinase
recombinant, expressed in Sf9 cells
Catalog Number C0624
Lot Number 050M0859
Storage
c-KIT (544-end, V654A) Active (C0499) - Datasheet
c-KIT (544-end, V654A), Active, GST-tagged, human
Precisio Kinase
recombinant, expressed in Sf9 cells
Catalog Number C0499
Lot Number 109K0816
c-KIT (544-end, V654A), active (C0499) - Datasheet
c-KIT (544-end, V654A), active, GST-tagged, human
PRECISIO Kinase
recombinant, expressed in Sf9 cells
Catalog Number C0499
Lot Number 041M0969
c-KIT (544-end) Active (C0624) - Lot 041M0985 - Datasheet
c-KIT (544-end), active, GST-tagged, human
PRECISIO Kinase
recombinant, expressed in Sf9 cells
Catalog Number C0624
Lot Number 041M0985
Hyunwook Jung, Arch Plast Surg. 47(4):
297-300 (2020)
5. Holmbeck, K.; Birkedal-Hansen, H.
6. Encyclopedia of Biological Chemistry, 542-544 (2013)
7. Harold E. Van Wart, in Handbook of Proteolytic
Immunol. 6;31(1):
51-56 (2019)
4. Holmbeck, K.; Birkedal-Hansen, H.
5. Encyclopedia of Biological Chemistry,
542-544 (2013)
6. Harold E. Van Wart, in Handbook of Proteolytic
Enzymes (Third
Kunitz, M., J. Gen. Physiol., 33(4),
349-362 (1950).
5. Holmbeck, K.; Birkedal-Hansen, H.
6. Encyclopedia of Biological Chemistry,
542-544 (2013)
7. Harold E. Van Wart, in Handbook of Proteolytic
8. Encyclopedia of Biological Chemistry, 542-544
(2013)
9. Harold E. Van Wart, in Handbook of Proteolytic
Enzymes (Third Edition),607-611 (2013)
10. Brondon Y.H.Chan et al, Encyclopedia of
solution Light amber
Appearance in solution Opalacent
pH (5% in water) 5.0-6.0
Loss on drying (Moisture) ≤10%
Sulfated ash ≤5%
Heavy (toxic) metals (as Pb) 0.0005%
Ca ≤0.1%
Mg ≤0.05%
1992), pp. 544-546.
4. Grover, P. K., et al., Dissolved urate salts out
calcium oxalate in undiluted human urine in vitro.
Implications for calcium oxalate stone genesis.
Chem. Biol., 10(3), 271-278
buffer, pH 8.0. Add 0.25 mL DTNB (5’,5’-dithio-bis(2-nitrobenzoic acid),
D8130) solution. Bring the volume to 10 mL with 0.1 M Tris, pH 8.0 (note the exact volume). Shake 10
min. Filter or centrifuge to
Immunoprecipitation: a working amount of 10-20 µg is
recommended using lysates of HEK-293T cells over-
expressing human dysbindin (DTNBP1).
Immunofluorescence: a working concentration of
5-10 µg/mL is recommended using
Biochemistry with Clinical
Correlations, Devlin, T. M., ed., Wiley-Liss (New
York, NY: 1992), pp. 544-546.
5. DNA Replication, 2nd ed., Kornberg, A., and
Baker, T. A., W. H. Freeman and Co. (New York,
NY
et al., Cyclophilin: a specific
cytosolic binding protein for cyclosporin A. Science,
226(4674), 544-547 (1984).
4. Wesselborg, S. et al., Identification of a physical
interaction between calcineurin
properties of
ascochlorin. (Studies on antiviral and antitumor
antibiotics. I). J. Antibiot., 21, 539-544 (1968).
2. Takatsuki, A. et al., Screening of antiviral antibiotics
from actinomycetes: correlation
Arch. Gen. Psychiatry, 61,
544-555 (2010).
3. Talbot, K., et al., J. Clin. Invest., 113, 1353-1363
(2004).
4. Takao, K., et al., Mol. Brain, 1,11- (2008).
5. Ghiani, C.A., et al.
properties of
ascochlorin. (Studies on antiviral and antitumor
antibiotics. I). J. Antibiot., 21, 539-544 (1968).
3. Takatsuki, A. et al., Screening of antiviral antibiotics
from actinomycetes: correlation
a
PVP-free polycarbonate filter (5 m pore size) and
0.2 10
4
cells/well was added to the top chamber. After
incubation for 3 hours at 37 C in a 5% CO2 humidified
incubator, the
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Anti-AtNOS1 (C-terminal) is developed in rabbit using a
synthetic peptide corresponding to amino acids 544-
561 located at the C-terminus of AtNOS1, conjugated to
KLH, as immunogen. The antibody is affinity-purified
Natl.
Acad. Sci. USA, 69, 1430 (1972).
10. Lachaael, M., et al., J. Biol. Chem., 271, 5225
(1996).
11. Shepro, D., et al., J. Cell Biol., 47, 544 (1970).
12. White, J.G., Roussel Conference
the lower chamber are then
read in a 96-well spectrofluorimeter with excitation
wavelength at 544 nm and emission at 590 nm.
The ND50 is the concentration of antibody required to
yield one-half maximal
547 reactive is an aminoreactive fluorescent biolabel designed for excitation by He/Ne lasers (at
544 nm) or mercury arc lamps. It can be used for covalent coupling to proteins and other biomolecules containing