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13. European
ratio (F/P) of the product: 3.0 to 9.0
Spectral Characteristics of Cy3:15
Absorbance Max = 552 nm
Emission Max = 570 nm
Precautions and Disclaimer
Due to the sodium azide content
Rubio, FJ, et al., Gene Therapy (1999) 6:1851-1866.
13) Gould, E., et al., Science (1999) 286:548-552.
14) Nichols, M., et al., Science (1999) 286:1558-1561.
15) Justicia, C. et al., Glia (2000) 30
Determination of Dye to Protein Molar Ratio
(D/P ratio)
1. Measure the Cy3 and Cy5 absorbance at 552 nm
and 650 nm, respectively. Read the absorbance of
the dyes at their exact absorbance wavelength
Determination of Dye to Protein Molar Ratio (D/P
ratio)
1. Measure the Cy3 and Cy5 absorbance at 552 nm
and 650 nm, respectively. Read the absorbance of
the dyes at their exact absorbance wavelength
bgh) polyadenylation signal is patented under U.S. Patent No.
5,122,458, European Patent No. 0 173 552, and Japanese Patent No. 6-83669. Use of the bgh
polyadenylation signal found in screening systems
Determination of dye to protein molar ratio (D/P
ratio).
1. Measure the Cy3 and Cy5 absorbance at 552 nm
and 650 nm, respectively. Read the absorbance of
the dyes at their exact absorbance wavelengths
Determination of Dye to Protein Molar Ratio
(D/P ratio)
1. Measure the Cy3 and Cy5 absorbance at 552 nm
and 650 nm, respectively. Read the absorbance of
the dyes at their exact absorbance wavelength
Activation of programmed cell death
in the rat ventral prostate after castration. Endocrin. 122: 552-562.
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Application of biotin, digoxigenin
Activation of programmed cell death
in the rat ventral prostate after castration. Endocrin. 122: 552-562.
22. Li, W., W.M. James, F. Traganos and Z. Darzynkiewicz. (1995)
Application of biotin, digoxigenin
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American Public Health Assn., Washington , D.C. pp. 539-552
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for Viral, Rickettsial
TdT) assay for the flow
32
cytometric analysis of apoptosis in drug resistant and drug sensitive
leukemia cells. Cytometry 20:245-256.
9. Davidson, F.D., M.
utilizing energy transfer primers: comparison with gel- and ELISA-based detection.
Biotechniques 26 (3):552-558.
20
Disclaimers
SYBR® is a registered trademark of Molecular Probes.
utilizing energy transfer primer: Comparison with gel- and ELISA-based detection.
Biotechniques 26:552-558.
12. Okayama, H., D. Curiel, M. Brantly, M. Holmes, and R. Crystal. 1989. Rapid, nonradioactive
utilizing energy transfer primer: Comparison with gel- and ELISA-based detection.
Biotechniques 26:552-558.
12. Okayama, H., D. Curiel, M. Brantly, M. Holmes, and R. Crystal. 1989. Rapid, nonradioactive
utilizing energy transfer primer: Comparison with gel- and ELISA-based detection.
Biotechniques 26:552-558.
12. Okayama, H., D. Curiel, M. Brantly, M. Holmes, and R. Crystal. 1989. Rapid, nonradioactive
structure of virus
filters using scanning electron microscopy with gold
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Parenteral Drug Association, Bethesda
Anti Cy3+Cy5 C0992 NA NA M
517 Nitrotyrosin N0409 NA NA P n/d n/d n/d
528 Anti Cy3+Cy5 C0992 NA NA M
552 Anti Cy3+Cy5 C0992 NA NA M
565 Phosphoserine P5747 NA NA M
566 Phosphothreonine P6623 NA NA M
567
Anti Cy3+Cy5 C0992 NA NA M
517 Nitrotyrosin N0409 NA NA P n/d n/d n/d
528 Anti Cy3+Cy5 C0992 NA NA M
552 Anti Cy3+Cy5 C0992 NA NA M
565 Phosphoserine P5747 NA NA M
566 Phosphothreonine P6623 NA NA M
567
Anti Cy3+Cy5 C0992 NA NA M
517 Nitrotyrosin N0409 NA NA P n/d n/d n/d
528 Anti Cy3+Cy5 C0992 NA NA M
552 Anti Cy3+Cy5 C0992 NA NA M
565 Phosphoserine P5747 NA NA M
566 Phosphothreonine P6623 NA NA M
567