determine optimal working dilutions by titration.
References
1. Nusse, R., Trends Genet., 15, 1-3 (1999).
2. Katoh, M., Curr. Drug Targets, 9, 565-570 (2008).
3. Semenov, M., and Snyder, M., Genomics, 42
0.408 565
OB
0 450 µg/l DEHA 1 inch B) 562
0.027 0.667 mg/l Carbohy 20 mm 0.544 565
0 600 µg/l Carbohy 1 inch B) 562
0.053 1.315 mg/l Hydro 20 mm 1.073 565
wavelength at 470-495 nm (FLIPRTETRA) or 485 nm (FLIPR1,
FLIPR2, FLIPR3) and emission wavelength at 515-565 nm (FLIPRTETRA) or
emission filter for Ca2+ dyes (FLIPR1, FLIPR2, FLIPR3). Set pipet tip height at
To read on the Erenna® Immunoassay System
1. Add 10 µL per well of Buffer C using reverse pipetting to Erenna® reading plate
(Fisher Scientific PN 12-565-384) using a 12-channel manual P20.
2. Transfer
To read on the Erenna® Immunoassay System
1. Add 10 µL per well of Buffer C using reverse pipetting to Erenna® reading plate
(Fisher Scientific PN 12-565-384) using a 12-channel manual P20.
2. Transfer
Burlington MA 01803, USA, Tel. +1-978-715-4321
Sigma-Aldrich Canada Co. or Millipore (Canada) Ltd.
2149 Winston Park, Dr. Oakville, Ontario, L6H 6J8
Phone: +1 800-565-1400
7.75327.0005
15
References
1. Giuliano KA, Haskins JR, Taylor DL. Advances in high content screening for drug discovery.
Assay Drug Dev Technol. 2003;1:565–577. PMID: 15090253
2. Giuliano
Nitrotyrosin N0409 NA NA P n/d n/d n/d
528 Anti Cy3+Cy5 C0992 NA NA M
552 Anti Cy3+Cy5 C0992 NA NA M
565 Phosphoserine P5747 NA NA M
566 Phosphothreonine P6623 NA NA M
567 Phosphotyrosine P1869 NA NA
Nitrotyrosin N0409 NA NA P n/d n/d n/d
528 Anti Cy3+Cy5 C0992 NA NA M
552 Anti Cy3+Cy5 C0992 NA NA M
565 Phosphoserine P5747 NA NA M
566 Phosphothreonine P6623 NA NA M
567 Phosphotyrosine P1869 NA NA
Nitrotyrosin N0409 NA NA P n/d n/d n/d
528 Anti Cy3+Cy5 C0992 NA NA M
552 Anti Cy3+Cy5 C0992 NA NA M
565 Phosphoserine P5747 NA NA M
566 Phosphothreonine P6623 NA NA M
567 Phosphotyrosine P1869 NA NA
72 Anti Cy3+Cy5 C0992 M NA NA NA
73 CDK5 C6118 M y y n/d
74 Bmf, N-Terminal B1684 P y y n/d
75 Bmf, C-Terminal B1559 P y y n/d
76 BNIP3 B7931 M y n/d n/d
77 BOB.1/OBF.1 B7810 M y y n/d
78