photometrically
at 580 nm.
https://www.sigmaaldrich.com
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Roche Diagnostics GmbH
Sandhofer Strasse 116
68305 Mannheim
Germany
4. Supplementary
excitation and
emission wavelengths of Mito Red are 560 nm and 580 nm, respectively.
Product Description
Chemical Name: 9-[2-(4'-Methylcoumarin-7'-oxycarbonyl)phenyl]-3,6-
bis(diethylamino
Standard wells 1-4.
3. At the end of the incubation period, quickly add
100 µL of Reagent B to stop the reaction. Tap
plate to mix.
Measurement
Read the optical density (OD) at
. Toxicol., 15(11), 1451-1459 (2002).
4. Olczak, A. A., et al, Association of Helicobacter
pylori Antioxidant Activities with Host Colonization
Proficiency. Infect. Immun., 71(1), 580-583 (2003).
during extractions from animal and
plant tissues or cells, yeast, and bacteria
Cat. No. 11 873 580 001 20 tablets in a glass vial
for 50 ml each
Cat. No. 05 056 489 001 3 x 20 tablets
. Toxicol., 15(11), 1451-1459 (2002).
4. Olczak, A. A., et al, Association of Helicobacter
pylori Antioxidant Activities with Host Colonization
Proficiency. Infect. Immun., 71(1), 580-583 (2003).
digitonin-permeabilized
HeLa cells was observed at a concentration of
120 µg/ml.
λex 560 nm; λemm 580 nm
References
1. Moore, M.S., and Blobel, G., Proc. Nat. Acd. Sci.
USA, 91, 10212-10216
2004).
2. Schraen-Maschke, S. et al., Biomark. Med., 2(4),
363-384 (2010).
3. Zilka, N. et al., FEBS Lett., 580(15), 3582-3588
(2006).
4. Christensen, R.A. et al., J. Biol. Chem., 279(51)
MBD2 and MBD3,
sequence similarity is limited to the methyl-CpG binding
domains (MBD). Human MBD4, (580 amino acids),
contains an N-terminal MBD domain and a distinctive
C-terminal glycosylase domain.