wells 1-4.
3. At the end of the incubation period, quickly add
100 µL of Reagent B to stop the reaction. Tap
plate to mix.
Measurement
Read the optical density (OD) at 580
Anti-phospho-PYK2 (pTyr579/580)
Developed in Rabbit, Affinity Isolated Antibody
Product Number P 6989
Storage Temperature −20 °C
Product Description
Anti-phospho-Pyk2 (pTyr579/580) was developed in rabbit
(2010).
3. Zilka, N. et al., FEBS Lett., 580(15), 3582-3588
(2006).
4. Christensen, R.A. et al., J. Biol. Chem., 279(51),
53524-53532 (2004).
5. Borroni, B. et al., Neurology, 71(22), 1796-1803
Zhou, M., et al., Biochem. Biophys. Res. Commun.,
280, 574-580 (2001).
2. Nath, D., et al., J. Cell Sci., 113, 2319-2328 (2000).
3. Weskamp, G., et al., J. cell Biol., 132, 717-726
(1996).
Zhou, M., et al., Biochem. Biophys. Res. Commun.,
280, 574-580 (2001).
2. Nath, D., et al., J. Cell Sci., 113, 2319-2328 (2000).
3. Weskamp, G., et al., J. cell Biol., 132, 717-726
(1996).
Zhou, M., et al., Biochem. Biophys. Res. Commun.,
280, 574-580 (2001).
2. Nath, D., et al., J. Cell Sci., 113, 2319-2328 (2000).
3. Weskamp, G., et al., J. cell Biol., 132, 717-726
(1996).
columns at linear flow rates of 78 cm/hour or
580 cm/hour respectively.
Pressure drops were reproducibly found to be as low as 0.5 bar at
flow rates of 580 cm/hour.
F
9
https://www.sigmaaldrich.com
3. Troubleshooting
y Version: 22 Restriction Endonuclease Xba I from Xanthomonas campestris
6 sigma-aldrich.com
3. Troubleshooting
Observation Possible cause
. et al., J. Am. Chem. Soc., 127(3),
1038-1048 (2005).
14. Calligaris, S. et al., Pediatr. Res., 62(5), 576-580
(2007).
15. Hiyane, R. et al., Ann. Bot., 106(3), 395-403
(2010).
16. Hong
Aβ40 [3].
BTA-1 is a fluorescent Thioflavin-T (Prod. No. T 3516) derivative.
The compound exhibits high affinity for synthetic aggregated
Aβ40 fibrils, displaying a Ki value of 11 nM vs. 580 nM for
carboxyfluorescein (FAM; lex 494 nm;
lem 518 nm) and carboxytetramethyl-rhodamine (TAMRA; lex
555 nm; lem 580 nm) are supplied as single isomers, ensuring
labeled products of defined chemical structure, as well