CN
585-50-2
应用筛选条件
关键词:'585-50-2'
显示 1-30 共 203 条结果 关于 "585-50-2" 范围 技术文档
Product Information Sheet - 11585622910
11 585 649 910
CSPD, ready-to-use 2 × 50 ml 11 755 633 001
CDP-Star, ready-to-use 2 × 50 m 12 041 677 001
DIG-High Prime 160 �l 11 585 606 910
High Prime 200 �l 11 585 592 910
Hybridization bags 50 bags
Product Information Sheet - 11585592001
Prime DNA Labeling Kit 1 kit for 50 reactions 11 585 584 910
DIG DNA Labeling Kit 1 kit for 40 reactions 11 175 033 910
DIG Luminescent Detection Kit 1 kit for 50 blots 11 363
Data Sheet - 79454
information sheet
Product Details
Spectral data: λex = 571 nm / λem = 585 nm
Content: Sufficient for 50
Product Information Sheet - 11585584001
200 �l 11 585 592 001
DIG-High Prime 160 �l 11 585 606 910
Fluorescein-High Prime 100 �l 11 585 622 910
Biotin-High Prime 100 �l 11 585 649 910
Quick Spin Columns,
Sephadex G-50 (Fine)
Product Information Sheet - MAK520
Working Reagent to all wells.
2. Briefly tap plate to mix.
3. Incubate for 20 min in the dark.
4. Read fluorescence intensity at λex = 530 nm
and λem =
Product Information Sheet - MAK522
mins at Room Temperature.
2. Read the optical density at 570nm (550-585 nm)
Fluorometric Procedure
1. Prepare a 200 µg/mL Standard by diluting 5 µL of
50 mg/mL Standard with 1.245 mL
MAK433 Technical Bulletin
MAK433D 1 L 1 L
Assay protocol:
1. Add 50 L of sample to each sample well.
2. Add 50 L of sample to each sample blank
well.
3. Add 50 L
Product Information Sheet - 11442066001
NBT crystals 5 g 11 585 029 001
Labeling of Biomolecules
Product Pack size Cat. No.
DIG RNA Labeling Mix 40 �l
(20 reactions)
11 277 073 910
DIG DNA Labeling Mix 50 �l
(25 reactions)
Product Information Sheet - MAK460
Measurement
1. Quickly add 50 L of Working Reagent to
each well and tap plate to mix.
2. Immediately measure the fluorescence
intensity at Ex = 530 nm/Em = 585 nm
for time zero (F0).
Product Information Sheet - MAK121
530/λem = 585 nm)
product directly proportional to neuraminidase activity
in the sample. The linear detection range at 37 C is
0.1–10 units/L for colorimetric assays and
0.01–2 units/L
Product Information Sheet - MAK332
530 nm/em = 585 nm is directly proportional to the
pyruvate concentration in the sample.
The Pyruvate Assay Kit has a linear detection range for
pyruvate in a 96 well plate assay of 2–500 M
Product Information Sheet - MAK321
Master Reaction Mixes according to the
scheme in Table 2. 50 µL of the appropriate
Master Reaction Mix is required for all wells.
Table 2.
Master Reaction Mixes
Reagent
Standards
Product Information Sheet - MAK478
peroxide concentration is
determined with a colorimetric reagent at 585 nm.
The linear detection range of the kit is
0.3 – 50 units/liter (U/L) α-amylase. The kit is
suitable for the determination
Product Information Sheet - MAK502
for 20 minutes at
room temperature.
2. Measure optical density at 570 nm (550-585 nm)
or fluorescence intensity at
λEx = 530 nm/ λEm = 585 nm.
Product Information Sheet-11096176001
3 μg DNA and
detection of 24 blots of 100 cm2
11 585 614 910
Buffers in a Box, Premixed SSC
Buffer, 20x
4 l 11 666 681 001
1 2 3
1 2 3
Product Information Sheet - MAK500
minutes at
37 °C or 60 minutes at RT.
2. Measure the optical density (OD) at 570 nm for
colorimetric assay or measure fluorescence at
λem = 585 nm/λex = 530 nm
Data Sheet - S4501
yield
reproducible gels.
References
1. Smithies, O.: Biochem. J., 61, 629 (1955)
2. Smithies, O.: Biochem. J., 71, 585 (1959)
MMS 7/21/98
Sigma brand products are sold through Sigma-Aldrich, Inc
Product Information Sheet - MAK331
Procedure 10-fold in Assay Buffer.
2. Transfer 50 L of diluted standards and 50 L of
diluted samples into separate wells of a black
96 well plate.
3. Add 50 L of Reaction Mix (see
Product Information Sheet - MAK537
fluorescence intensity at
λex/λem = 530/585 nm.
The linear detection range of the kit is 0.86 to 500 µM
for the colorimetric assay and 0.18 to 50 µM for the
fluorometric
Product Information Sheet - MAK506
fluorescence
intensity at λEx =530 nm/λEm =585 nm is a direct
measure of the enzyme activity.
The linear detection range of the kit is 2 to 50 U/L
peroxidase for colorimetric
Data Sheet - 41629
applicable for 2 different protein concentration ranges.
1. Low range (linear range 50 µg/ml Protein):
The following solutions are required:
1. 50 mM Phosphate buffer (pH 5.0)
2. 0.1 % SDS solution
Product Information Sheet - MAK136
resulting in the formation of H2O2,
which is determined by a fluorimetric method
(ex = 530/em = 585 nm). The assay is simple,
sensitive, stable, and high-throughput adaptable. Unit
definition: one
Product Information Sheet-11585606910
available in US
11 666 916 001
CSPD, ready-to-use 2 x 50 ml 11 755 633 001
DIG-labeled Control DNA 50 μl, 5 μg/ml DIG-labeled plasmid DNA 11 585 738 910
DIG-High Prime DNA Labeling and
Detection Starter
Product Information Sheet-pefbsc-ro
523 001
25 mg 11 585 681 001
cOmplete 20 tablets in a glass vial, for 50 ml each 11 697 498 001
3 x 20 tablets in glass vials, for 50 ml each 11 836 145 001
20 tablets
Data Sheet - E1158
EPHB3 (585-end) Active (E1158) - Datasheet
EPHB3 (585-end) Active
human, recombinant
GST-tagged, expressed in Sf9 cells
Catalog Number E1158
Lot Number 019K1573
Storage Temperature –70 °C
Product Information Sheet - MAK443
product, measured at
Ex = 530 nm/Em = 585 nm, is proportional
to the lactate concentration in the sample.
The assay method has a linear response up
to 50 µM L-lactate and a detection limit of
1
Product Information Sheet - MAK436
Em = 585 nm is directly proportional to the
total cholesterol concentration in the sample.
The linear detection range for the assay
method is 0.1 – 10 mg/dL by colorimetric
detection or 0.02 – 2 mg/dL
Product Information Sheet - MAK516
60 µL Working Reagent to all sample and
inhibitor wells.
2. Tap plate briefly to mix.
3. Read fluorescence λex=530 nm / λem 585 nm
at 0 min (∆R0) and 10
Product Information Sheet - MAK531
. Tap plate to mix. Incubate
for 10 minutes.
2. Read optical density at 570nm (550 to 585nm) or
fluorescence intensity at λem/ex = 585/ 530nm.
114538, 114539, 114679, 114680, 114681, 114682 - Spectroquant COD solutions A%26#43;B
Solution B Cat. No. 114681 114682 114539 114680
Measuring wavelength 340 445 or 446 605 or 585 605 or 585 (nm)
Number of Solution A 210 210 210 225
determinations Solution B 170 170 210 275
1/7