Extraction Kit is from 400 bp to
5 kb with a reproducible recovery of about 80%.
Typical recoveries with smaller and larger fragment sizes:
• 20 bp – 55%
• 40 bp – 68%
• 120 bp – 76%
• 200 bp – 80%
• 8 – 9
USA 100 (6): 3324-3327.
Further Readings 5 Vogelstein, B. et al. (1979) Preparative and analytical purification of
DNA from agarose Proc Natl Acad Sci USA 76 (2):615-619.
6 Aitken, N. et al. (2004)
Reagents
PANSORBIN® Cells IP 507858 1 g
5 g
10 g
25 g
50 g
€77
€306
€�86
€965
€1700
PANSORBIN® Cells, Lyophilized EIA, IP, RIA 507862 1 g
5 g
€76
€291
PANSORBIN® Cells, Standardized IP
NP_001001655.1 y n/d n/d
755 Vesicular GABA Transporter V5764 140679 SLC32A1 P NP_542119.1 n/d y y
586 Protein Kinase Cδ P8333 170538 Prkcd P NP_579841.1 n/d n/d y
686 Spred-2 S7320 200734 SPRED2 P NP_001121682.1
NP_001001655.1 y n/d n/d
755 Vesicular GABA Transporter V5764 140679 SLC32A1 P NP_542119.1 n/d y y
586 Protein Kinase Cδ P8333 170538 Prkcd P NP_579841.1 n/d n/d y
686 Spred-2 S7320 200734 SPRED2 P NP_001121682.1
NP_001001655.1 y n/d n/d
755 Vesicular GABA Transporter V5764 140679 SLC32A1 P NP_542119.1 n/d y y
586 Protein Kinase Cδ P8333 170538 Prkcd P NP_579841.1 n/d n/d y
686 Spred-2 S7320 200734 SPRED2 P NP_001121682.1
.70
BSB-Me 505 92±3 1.0 9.1 0.78±0.10 2.66
BSB-OMe 530 90±1 1.2 7.2 0.90±0.10 2.33
BSB-CN 586 55±2 1.5 3.7 2.35±0.10 1.84
The ASE thresholds are not directly correlated with φf or τf but are
P3203 P n/d n/d y
584 Protein Kinase Cβ2 P2584 M n/d n/d y
585 Protein Kinase Cγ P8083 M n/d n/d y
586 Protein Kinase Cδ P8333 P n/d n/d y
587 Protein Kinase Cε P8458 P n/d n/d y
588 Protein Kinase Cζ
dioxane sol. 160 g TCA, 8 mL 25%
ammonia in 1 L water; 70/30
Migration distance 7 cm
Detection 586 nm (TLC/HPTLC Scanner, Camag)
Separation of oligo-nucleotides
www.merck-chemicals.com/thin-layer-chromatography
25023 Prkcb1 NP_036845.2 M n/d n/d y
585 Protein Kinase Cγ P8083 24681 Prkcc NP_036760.1 M n/d n/d y
586 Protein Kinase Cδ P8333 170538 Prkcd NP_579841.1 P n/d n/d y
587 Protein Kinase Cε P8458 29340 Prkce
25023 Prkcb1 NP_036845.2 M n/d n/d y
585 Protein Kinase Cγ P8083 24681 Prkcc NP_036760.1 M n/d n/d y
586 Protein Kinase Cδ P8333 170538 Prkcd NP_579841.1 P n/d n/d y
587 Protein Kinase Cε P8458 29340 Prkce