Immobilon-P membrane
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filter paper
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PR02534_RevB.indd 1 12/4/2008 9:59:40 AM
Materials Recommended for Western Blotting
■ Immobilon-P Blotting Sandwich
■ Transfer
Recognition Sequence: 5’C/CGG’3
Activity: 10,000 units/ml
Cutting: 100%
Ligation: >95%
Recutting: >95%
No degradation detected with >40 units for 16 hrs.
Fold over digestion: 640 (40 units x 16 hrs.
Determinants for
Heparin Binding in Human Coagulation Factor XI”.
Temple University, Ph.D. dissertation, p. 59
(2008).
10. Dean, Sadie, “3-Nitrotyrosine As An Indicator Of
The Disease State Intermittent
Ala 30
Arg 31
Asp 67
Cys 3
Glu 186
Gly 19
His 22
Ile 44
Leu 75
Lys 68
Met 27
Phe 40
Pro 88
Ser 51
Thr 42
Trp 10
Tyr 28
Val 59
The total amino acid content is approximately
890 mg/g.
This product is
ethanol at 20 °C)1
The CMC is 0.21% (5 mM) and it is considered to be
readily dialyzable.3
Deoxycholic acid has been used in a modified
procedure to recover 40-80% of a protein from a
1 µg/ml solution
elemental content.
The CMC is 0.21% (5 mM) and it is considered to be
readily dialyzable.3
Deoxycholic acid has been used in a modified
procedure to recover 40-80% of a protein from a
1 µg/ml solution