Cleavage of the peptide by
renin results in the production of a fluorescent product
(λex = 540/λem = 590 nm). This assay is ∼50-fold more
sensitive than the EDANS/DABCYL-based assay.
Some crude biological
constants of biologically active, intercalating
fluorescent stains. Anal. Biochem., 95(2), 329-339
(1979).
KTA 01/06-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
coupled enzyme assay,
which results in a colorimetric (570 nm)/
fluorometric (ex = 535/em = 590 nm) product,
proportional to the fatty acids present.
Components
The kit is sufficient for
Tauhata, S.B.F., et al., J. Biol. Chem., 276, 39812
(2001).
7. Mehta, A.D., et al., Nature, 400, 590 (1999).
8. Rief, M., et al., Proc. Natl. Acad. Sci. USA
read in the multiwell plate reader at 405 nm with
corrections at 570 or 590 nm.
c. Subtract the readings at 590 nm from the readings
at 405 nm, to correct for optical imperfection of the
absorbance
at 570 nm (A570). For fluorometric assays, measure
fluorescence intensity (λex = 535/λem = 590 nm).
3
Results
Calculations
The background for the assays is the value obtained for
Amount of newly synthesized
Bio-16-dUTP labeled DNA
after 1 hour
after 2 hours
after 5 hours
175 ng
300 ng
500 ng
590 ng
950 ng
1340 ng
1550 ng
DNA synthesis was measured by incorporation
mouse melanoma cell lines with defined genetic
alterations. Pigment Cell Melanoma Res 29(5): 590-597.
2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten
loss to induce metastatic melanoma
mouse melanoma cell lines with defined
genetic alterations. Pigment Cell Melanoma Res 29(5):
590-597.
2. Dankort D et al. (2009) Braf(V600E) cooperates with
Pten loss to induce metastatic melanoma
mouse melanoma cell lines with defined genetic alterations.
Pigment Cell Melanoma Res 29(5): 590-597.
2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten
loss to induce metastatic melanoma
mouse melanoma cell lines with defined genetic
alterations. Pigment Cell Melanoma Res 29(5): 590-597.
2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten
loss to induce metastatic melanoma
mouse melanoma cell lines with defined genetic
alterations. Pigment Cell Melanoma Res 29(5): 590-597.
2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten
loss to induce metastatic melanoma
equivalent)
• Microplate reader with absorbance filter suitable for measuring OD between 590
and 630 nm
• 2 ml, 5ml, 10 ml tubes
• MilliQ water (phosphate free water)
Section
5’OH
Recognition
Site 5’CCCTT3’
Vaccinia Topoisomerase
Cal Red 590
FAM
T4 DNA Ligase
Figure 2.
Assay Principle
It offers new dual-detection technology which labels both