fluorescence intensity at ex/em = 530/590 nm is
directly proportional to glycerol concentration in the
sample.
Detection ranges and limits
Linear detection range: 2 -100 μM, 18-920 μg/dL or
0.18-9.20 ppm.
Control Ranges for LINCO Research, Inc
HNDG4-6036-4: HNDG4-109.209 (18 November 2019)
EMD Millipore • 1-800-645-5476 • 1-800-645-5439 (FAX) • emdmillipore.com
The M logo is a trademark of Merck
wavelength
correction set at 590, 600, or 620 nm. If the absorbance readings exceed the
limitations of the plate reader, a second reading at 405 nm is needed (reference
filter 590, 600 or 620 if available)
with 1N HCl
References:
1. C.D. Jeffries, D.F. Holtmann, D.G. Guse, Rapid method for determining the activity of microorganisms on
nucleic acid, J. Bact., 73, 590-591 (1957)
2. B.G. Weckman
Tauhata, S.B.F., et al., J. Biol. Chem., 276, 39812
(2001).
7. Mehta, A.D., et al., Nature, 400, 590 (1999).
8. Rief, M., et al., Proc. Natl. Acad. Sci. USA, 97,
Sample Volume
F = Dilution Factor from sample preparation
REFERENCES:
1. Bondar, R.J.L. and Mead, D.C., Clin.Chem. 20, 586-590 (1974)
2. Kunsst, A., Draeger, B. and Ziegenhorn, J., Methods of Enzymatic
green fluorescence at 527 nm.
• However, when the concentration is > 1 mM,
a very strong red-orange fluorescence will
occur at 590 nm.
This is due to the formation of aggregates of the dye,
named
Microbiology.book
590 Merck Microbiology Manual 12th Edition
MAS-100 Eco®
The microbial airsampler system
The MAS-100 Eco® microbial airsampler is supplied with Mains
power supply unit
D.G.: Rapid method for
determining the activity of microorganisms on nucleic acid. - J. Bact., 73;
590-591 (1957).
SCHREIER, J.B.: Modification of Deoxyribonuclease Test Medium for rapid
identification
), 4172-
4183 (2016).
6. Chen, Q. et al., J. Biotech., 263, 30-35 (2017).
CMH,GCY,MAM 12/18-1
2018 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
), 4172-
4183 (2016).
6. Chen, Q. et al., J. Biotech., 263, 30-35 (2017).
CMH,GCY,MAM 12/18-1
2018 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
absorbency
at 450 nm and 590 nm
• Orbital Microtiter Plate Shaker
• Absorbent Paper or Cloth
Sample Collection and Storage
Preparation of Serum Samples
1.
absorbency
at 450 nm and 590 nm
• Orbital Microtiter Plate Shaker
• Absorbent Paper or Cloth
Sample Collection and Storage
Preparation of Serum Samples
1.
granulation: The influence of mannitol grade on granule and tablet properties.
Int. J. Pharm. 2020, 590, 119890.
Figure 2.
Friability (top) and fines (bottom) in function of L/S-ratio for formulations
capable of reading absorbency at 450 nm
and at 590 nm
• Orbital Microtiter Plate Shaker
• Absorbent Paper or Cloth
Sample Collection and Storage
1. To prepare serum, whole blood is directly drawn
for use in diagnostic procedures.
IFU-EZRMI-13K Rev 02/24 18
Quality Controls
The ranges for each analyte in Quality Control 1 and 2 are provided on the card
insert, or available at our
developed in rabbit using as
immunogen a synthetic peptide corresponding to amino
acid residues 577-590 of human HDRP/MITR with
N-terminal added cysteine, conjugated to KLH. The
corresponding sequence