determined via a coupled enzymatic
reaction. The fluorescence intensity, measured at
λex = 535 nm/λem = 590 nm, is proportional to the
amount of methanol present in the sample.
The kit’s linear range
D.G.: Rapid method for
determining the activity of microorganisms on nucleic acid. - J. Bact., 73;
590-591 (1957).
SCHREIER, J.B.: Modification of Deoxyribonuclease Test Medium for rapid
identification
with 1N HCl
References:
1. C.D. Jeffries, D.F. Holtmann, D.G. Guse, Rapid method for determining the activity of microorganisms on
nucleic acid, J. Bact., 73, 590-591 (1957)
2. B.G. Weckman
green fluorescence at 527 nm.
• However, when the concentration is > 1 mM,
a very strong red-orange fluorescence will
occur at 590 nm.
This is due to the formation of aggregates of the dye,
named
Tamoxifen and estrogens as
membrane antioxidants: comparison with
cholesterol. Methods Enzymol., 234, 590-602
(1994).
11. Cardoso, C.M. et al., Comparison of the changes in
adenine nucleotides of rat
Tamoxifen and estrogens as
membrane antioxidants: comparison with
cholesterol. Methods Enzymol., 234, 590-602
(1994).
11. Cardoso, C.M. et al., Comparison of the changes in
adenine nucleotides of rat
read in the multiwell plate reader at 405 nm with
corrections at 570 or 590 nm.
c. Subtract the readings at 590 nm from the readings
at 405 nm, to correct for optical imperfection of the
samples. This kit has 100% crossreactivity to rat GIP and mouse GIP and
measures both GIP(1-42) and GIP(3-42). One kit is sufficient to measure 39 unknown
samples in duplicate. This kit is for Research
1992).
28. Powis, G. Trends Pharmacol. Sci. 12, 188, (1991).
29. Wiseman, H. Methods in Enzymol. 234, 590, (1994).
30. Martindale, The Extra Pharmacopoeia, 30th ed. 500, (1993).
31. Goodman and Gilman’s The
measured spectrophotometrically by the increased absorbency
at 450 nm, corrected from the absorbency at 590 nm, after acidification of formed
products. Since the increase in absorbency is inversely proportional
/Rat) 0%
Pancreaetic Polypeptide (Human, Rat) 0%
Human GIP (1-42) 0%
Human GIP (3-42) 0%
Human Insulin 0%
Human Leptin 0%
Human GLP-1 0%
Human C-peptide 0%
Human Amylin 0%
Glucagon 0%
Rat/Mouse
measured spectrophotometrically by the increased absorbency
at 450 nm, corrected from the absorbency at 590 nm, after acidification of formed
products. Since the increase in absorbency is inversely proportional
nm and 590 nm in a plate reader within 5 minutes and ensure that there
are no air bubbles in any well. Record the difference in absorbance units. The
absorbance of the highest Amyloid β 1-42 standard
nm and 590 nm in a plate reader within 5 minutes and ensure that there are no air
bubbles in any well. Record the difference in absorbance units. The absorbance of
the highest Amyloid β 1-42 standard
450 nm and 590 nm in a plate reader within 5 minutes and ensure that there
are no air bubbles in any well. Record the difference in absorbance units. The
absorbance of the highest Amyloid β1-42 standard
nm and 590 nm in a plate reader within 5 minutes and ensure that there are no air
bubbles in any well. Record the difference in absorbance units. The absorbance of
the highest Amyloid β 1-42 standard
tissue extracts and cell culture
samples. This kit has 100% cross reactivity to human GIP (1-42) and GIP (3-42). One
kit is sufficient to measure 39 unknown samples in duplicate. This kit is for Research
Pancreatic Polypeptide (Human, Rat) 0%
Human GIP (1-42) 0%
Human GIP (3-42) 0%
Human Insulin 0%
Human Leptin 0%
Human GLP-1 0%
Human C-peptide 0%
Human
) and fluorescence microscopy images of Catalog No. CLL1035 (ex 530-560/em 590-650, 40×/1.4 oil).
6
References
1. Centers for Disease Control, Biosafety in
Microbiological and Biomedical
samples. This kit has 100% cross reactivity to rat GIP and mouse GIP
and measures both GIP(1-42) and GIP(3-42). One kit is sufficient to measure
39 unknown samples in duplicate.
This kit is for