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wash 4 times as directed
in step 3.
6. Add 100 µL of prepared HRP-Streptavidin solution
(see Reagent Preparation step 11) to each well.
Incubate for 45 minutes with gentle shaking at room
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Recombinant catalytic domain of human MEG2 protein tyrosine phosphatase consisting of amino acids 285-593
fused to GST at the N-terminus, expressed in and purified from E. coli. PTP-MEG2 is an intracellular
Filter Paper (continued)
Whatman® filter paper
Flat grade 589/3 591 595 597 597 L 598 602 h 602 eh
Prepleated (Folded) grade 593½ 594½ 595 ½ 597 ½ 598 ½ 602 h½ 604 ½
Particle type
Coarse
Inhibitor IV
{3-Fluoro-N-(6,7-dimethoxy-2,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenylamine}
A cell-permeable, ATP-competitive and reversible inhibitor
of PDGFR tyrosine kinase (IC50 = 4.2 nM and 45 nM for
rat. Cell Proliferation 25: 241-250.
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