Buffer 90 µL 594 µL 1,188 µL
SmartLyse™ T
Protease 5 µL 33 µL 66 µL
Final Volume 95 µL 627 µL 1,254 µL
3. Place the reaction tube(s) in the thermal shaker
and incubate at 60
Buffer 90 µL 594 µL 1,188 µL
SmartLyse™ T
Protease 5 µL 33 µL 66 µL
Final Volume 95 µL 627 µL 1,254 µL
3. Place the reaction tube(s) in the thermal shaker
and incubate at 60
The entire sample can be drawn
onto the tooth in about 2−5 seconds.
5. After all samples have been loaded, wait
approximately 30-60 seconds to ensure that
the entire sample has been absorbed
585 amino acid residues) and is
encoded on human chromosome 10. GAD 67 is a
cytoplasmic protein (594 amino acid residues) and is
encoded on chromosome 2. There is 64% amino acid
identity between the
(585 amino acid residues) and is
encoded on human chromosome 10. GAD67 is a
cytoplasmic protein (594 amino acid residues) and is
encoded on chromosome 2. There is 64% amino acid
identity between the
to be 8.0 – 8.5.
Larger fragments (>5 kb) are adsorbed more
strongly to the matrix.
Elute at +56 to +60°C for 15 – 20 min.
Pellet is too dry. Elute at +56 to +60°C.
Inhibition of subsequent
enzymatic
(585 amino acid residues) and is encoded on human
chromosome 10. GAD67 is a cytoplasmic protein (594
amino acid residues) and is encoded on
chromosome 2. There is 64% amino acid identity
between the
585 amino acid residues) and is
encoded on human chromosome 10. GAD 67 is a
cytoplasmic protein (594 amino acid residues) and is
encoded on chromosome 2. There is 64% amino acid
identity between the
continuously
and record data every 5-10 minutes for
30-60 minutes, protected from light.
• For end-point reading: Incubate the reaction
at the desired temperature for 30-60 minutes,
protected from light
Reaction Tempera-
ture
Time
period
1 initial denatur-
ation
+95°C 5 min
30 denaturation
hybridization
elongation
+95°C
+60°C
+72°C
45 sec
1 min
2 min
final elongation +72°C up to 10
min