594-60-5

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High-level expression and characterization of a thermophilic β-mannanase from Aspergillus niger in Pichia pastoris.

Shi Yu et al.

Biotechnology letters, 37(9), 1853-1859 (2015-05-15)

A novel, high-level expression, thermostable mannan endo-1,4-beta-mannosidase is urgently needed for industrial applications. The mannan endo-1,4-β-mannosidase gene (MAN) from Aspergillus niger CBS 513.88 was optimized based on the codon usage bias in Pichia pastoris and synthesized by overlapping PCR to

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