Bradford Reagent and mix on a shaker for
∼ 30 seconds.
6. Let the samples incubate at room temperature
for 5–45 minutes. Then measure the absorbance
at 595 nm. The protein-dye complex is stable up
approximately 2 minutes, transfer the
solutions to separate cuvettes. Read and record
the absorbance (A) of Standard (ASTANDARD) and
Tests (ATEST) versus the Blank as the reference
at 595 nm. The color
Bradford
Reagent and mix on a shaker for ∼30 seconds.
6. Let the samples incubate at room temperature for
5–45 minutes. Then measure the absorbance at
595 nm. The protein-dye complex is stable up to
reaction and color reaction in one step. The change
in color intensity of the reaction product at 595 nm is
directly proportional to oxalate in the sample.
Sensitive and accurate – Use samples
reaction and color reaction in one step. The change in
color intensity of the reaction product at 595 nm is
directly proportional to oxalate in the sample.
Key Features
Sensitive and accurate – Use
270–81.e8 (2020).
7. Lan J., et al. Nature. 581:215–20 (2020).
8. Liu Z., et al. J Med Virol. 92:595–601 (2020).
9. Luan J., et al., Biochem Biophys Res Commun.
526:165–9 (2020).
10. Yan R., et
e8 (2020).
7. Lan J., et al. Nature. 581: 215–20 (2020).
8. Liu Z., et al. J Med Virol. 92: 595–601 (2020).
9. Luan J., et al., Biochem Biophys Res Commun.
526:165–9 (2020).
mM KH2PO4)
6. Deionized or distilled H2O.
7. 96-well microplate reader with 450-540 nm or 450-595 nm dual wavelength or 450 nm single
wavelength filter.
8. Tissue culture microplate (96 well
detection Detector for flammable gases
Safety data
Explosion Range 4,4 - 17 Vol.%
Ignition Temperature 595 °C
Materials
Cylinders and Valves: any usual materials
Seals: PTFE, PCTFE, PVDF, PA, PP, NBR, CR
Biochim. Biophys. Acta, 1477,
307-323 (2000).
2. Smith, M.J. et al., J. Leukocyte Biol., 60, 555-562
(1996).
3. Shresta, S. et al., Immunity, 10, 595-605 (1999).
4. Mullbacher, A. et al., Proc. Nat
20X and 1140 mL of
distilled water.
2. Mix and fill the washing device.
3. Store the washing buffer 1X at room temperature (15 – 30 °C) for 1 month or at 2 – 8 °C
1140 mL of
distilled water. Mix well.
2. Fill the washing device.
3. Store the washing buffer 1X at room temperature (15 – 30 °C) for 1 month or at 2 – 8 °C for a
P2X7 and is not present in any other known protein.
IMMUNOGEN: Purified peptide P2X7 from 576-595 of rat P2X7 (Accession Q64663).
APPLICATIONS: Western blot: 1:200-1:300 using ECL on rat brain
P2X7 and is not present in any other known protein.
IMMUNOGEN: Purified peptide P2X7 from 576-595 of rat P2X7 (Accession Q64663).
APPLICATIONS: Western blot: 1:200-1:1000 using ECL on rat brain
270–81.e8 (2020).
7. Lan J., et al. Nature. 581:215–20 (2020).
8. Liu Z., et al. J Med Virol. 92:595–601 (2020).
9. Luan J., et al., Biochem Biophys Res Commun.
526:165–9 (2020).
10. Yan R., et
SPECIFICITY: Recognizes Cav3.2 (α1H (T-Type)).
IMMUNOGEN: Purified peptide from amino acids 581-595 of rat Cav3.2 (Accession number Q9EQ60).
APPLICATIONS: Western blot: 1:200 using ECL on ND7/23
SPECIFICITY: Recognizes Cav3.2 (α1H (T-Type)).
IMMUNOGEN: Purified peptide from amino acids 581-595 of rat Cav3.2 (Accession number Q9EQ60).
APPLICATIONS: Western blot: 1:200 using ECL on ND7/23
date on
container.
2. Mix and fill the washing device.
3. The prepared 1X Washing Buffer solution may be stored at
room temperature (15 – 30 °C) for up to 1 month, or at 2 – 8
°C for up to 3 months
. Hoffmann, M. et al., Cell, 181(2), 271-280.e8
(2020).
8. Lan, J. et al., Nature, 581(7807), 215-220
(2020).
9. Liu, Z. et al., J. Med. Virol., 92(6), 595-601
(2020).
10. Luan, J.