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http://www.sigma-aldrich.com
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(i.e. 2 mL for 100 million cells).
19. Incubate on ice for 20 minutes and flick the tube occasionally to resuspend the cells.
20. Dounce up to 1 mL of the cell suspension on ice using a 2
AG25 H M WB IP IC Asc M IgG1 100 µL MAB3739
Protein
PTPMEG-2 KA 10 µg 14-592
LMPTP-B PA 10 µg 14-620
LMPTP-A PA 10 µg 14-619
PTPMEG-1 PA 10 µg 14-642
HePTP
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Jehličková
211 Cytokeratin peptide 19 C6930 M y n/d n/d
212 Pan Cytokeratin P2871 M y y y
213 DAPK D2178 M y n/d n/d
214 phospho-DAPK (pSer308) D4941 M y n/d n/d
215 DAP
n/d n/d
618 Pyk2 P3902 50646, 2185, 19229 Ptk2b,PTK2B NP_059014.2,NP_775268.1,NP_766086.1 P y y y
619 phospho-Pyk2 (pTyr579) P7114 50646, 2185 Ptk2b,PTK2B NP_059014.2,NP_775268.1 P y n/d y
620 phospho-