2.
Effects of possible interfering substances.
1. H2O, 2. Phosphate, 3. ATP in H2O,
4. ATP/Phosphate in H2O, 5, 7, 9, 11, 13:
ATP in Assay Buffer with, where indicated,
0.5 mg/mL BSA, 1 mM DTT, 2
of measuring absorbance of 96-well plates at wavelength of 620-630 nm, or
spectrophotometer (UV/Visible).
2. Incubator 30EC (If required).
3. Precision pipettes (2-20, 20-100 and 200-1000 :l) with disposable
mitochondrial functions
by the antibiotics, bikaverin and duclauxine. J.
Antibiot. (Tokyo), 31, 616-620 (1978).
5. Fuska, J. et al., New potential cytotoxic and
antitumor substances I. In vitro effect
precipitates, allowing TPTZ to
complex with Fe2+. The Fe-TPTZ complex results in a
colorimetric (620 nm) product proportional to the
chloride present.
Components
The kit is sufficient for 100 assays
at 450 nm (620 nm as optional reference wavelength)
- Glass-distilled or deionized water
- Statistical calculator with program to perform linear regression analysis.
3
monomeric.
References
1. Gross, A. et al., Genes and Develop. 13, 1899-
1911 (1999).
2. Kroemer, G., Nature Med., 3, 614-620 (1997).
Sigma brand products are sold through Sigma-Aldrich
New York (1993).
12. Cohn, E.J. et al., J. Am. Chem. Soc., 68, 459 (1946).
13. Saifer, A. and Goldman, L. J. Lipid Res., 2(3), 268 (1961).
14. Scott, T. and Eagleson, M., Concise Encyclopedia: Biochemistry
New York (1993).
12. Cohn, E.J. et al., J. Am. Chem. Soc., 68, 459 (1946).
13. Saifer, A. and Goldman, L. J. Lipid Res., 2(3), 268 (1961).
14. Scott, T. and Eagleson, M., Concise Encyclopedia: Biochemistry
New York (1993).
12. Cohn, E.J. et al., J. Am. Chem. Soc., 68, 459 (1946).
13. Saifer, A. and Goldman, L. J. Lipid Res., 2(3), 268 (1961).
14. Scott, T. and Eagleson, M., Concise Encyclopedia: Biochemistry
New York (1993).
12. Cohn, E.J. et al., J. Am. Chem. Soc., 68, 459 (1946).
13. Saifer, A. and Goldman, L. J. Lipid Res., 2(3), 268 (1961).
14. Scott, T. and Eagleson, M., Concise Encyclopedia: Biochemistry
New York (1993).
12. Cohn, E.J. et al., J. Am. Chem. Soc., 68, 459 (1946).
13. Saifer, A. and Goldman, L. J. Lipid Res., 2(3), 268 (1961).
14. Scott, T. and Eagleson, M., Concise Encyclopedia: Biochemistry
New York (1993).
12. Cohn, E.J. et al., J. Am. Chem. Soc., 68, 459 (1946).
13. Saifer, A. and Goldman, L. J. Lipid Res., 2(3), 268 (1961).
14. Scott, T. and Eagleson, M., Concise Encyclopedia: Biochemistry
New York (1993).
12. Cohn, E.J. et al., J. Am. Chem. Soc., 68, 459 (1946).
13. Saifer, A. and Goldman, L. J. Lipid Res., 2(3), 268 (1961).
14. Scott, T. and Eagleson, M., Concise Encyclopedia: Biochemistry