321-30 (1982).
4. Waymack, P.P., and van Etten, R.L., Isolation and
characterization of a homogeneous isoenzyme of
wheat germ acid phosphatase. Arch. Biochem.
Biophys., 288, 621-33 (1991).
5. Sugiura
321-30 (1982).
4. Waymack, P.P., and van Etten, R.L., Isolation and
characterization of a homogeneous isoenzyme of
wheat germ acid phosphatase. Arch. Biochem.
Biophys., 288, 621-33 (1991).
5. Sugiura
production of superoxide
radical. Biochem. Pharmacol., 37(2), 349-352
(1988).
4. Stryer, L., Biochemistry, 3rd ed., W.H. Freeman
(New York, NY: 1988), pp. 619-621.
5. Yang, J. J., and Finn, W. F., Effect of
305-46
(1989).
4. Waymack, P.P., and van Etten, R.L., Isolation and
characterization of a homogeneous isoenzyme of
wheat germ acid phosphatase. Arch. Biochem.
Biophys., 288, 621-33 (1991).
5. Brouillard
rapidly
and transferred to GHB broth to achieve a final
concentration of 4% (v/v) inoculum to broth. Cultures
were incubated at 37 °C (± 2 °C), with 6% (± 1%) CO2
for 22 (± 2) hours4.
For production
= 22.5 mL 67.5 mL/analysis
Total analysis/day 8 samples 32 samples 4-fold productivity increase
24
USP Chapter 621 - Chromatography
What changes are allowed in a monograph method?
. J. and MacDonald J. B., 1960, J. Bacteriol., 80:164.
9. Garrod, 1966, J. Pathol. Bacteriol., 91:621.
10. Lowrence and Traub, 1969, Appl. Microbiol., 17:839.
Precautions and Disclaimer
final volume of Reaction Mixture to 50 µl
3. Vortex the tubes briefly and incubate at 37 °C for
1 hour.
4. During the incubation, prepare the cleanup
columns. Mix the Cleanup Resin (Catalog Number
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
-
enrichment culture)
3. Incubate 24 h at 37°C
4. Transfer 0.1 mL first pre-enrichment culture to 10 mL fraser-boullion
5. Incubate 24 h at 37 °C
6. transfer a 2 mL
and homogenize for 1 minute in a Stomacher (first pre-enrichment
culture)
3. Incubate 4-6 h at 37°C
4. Incubate 44-48 h at 41,5 °C
5. Remove 0,2 mL of sample from the enrichment