340 and 341 and
serines 259 and 499 (activating), serine 43 (prevents
Ras:GTP binding), and serine 621(constitutive, required
for activity).6
Once active, Raf-1 phosphorylates and activates MAP
kinase
red screw
cap); mix and incubate for 15 minutes at 37°C in a thermoshaker
4. Add 50 µL of Lysis Buffer C (red cap) and incubate for 15 minutes at 37°C and 1,400 rpm in the
thermoshaker
red screw
cap); mix and incubate for 15 minutes at 37°C in a thermoshaker
4. Add 50 µL of Lysis Buffer C (red cap) and incubate for 15 minutes at 37°C and 1,400 rpm in the
thermoshaker
incubate for 15 minutes at 37°C in a thermoshaker
3. Add 450 µL of Lysis Buffer C (red cap) and incubate for 15 minutes at 37°C and 1,400 rpm in
the thermoshaker
4. Centrifuge for 10
2. J Biol Chem. 1995; 270(13): 7029-7032.
3. Science. 1998; 281(5375): 413-416.
4. Int J Cancer. 1977; 19(5): 621-626.
FOR RESEARCH USE ONLY.
NOT FOR USE IN DIAGNOSTIC PROCEDURES.
NOT FOR
red screw
cap); mix and incubate for 15 minutes at 37°C in a thermoshaker
4. Add 50 µL of Lysis Buffer C (red cap) and incubate for 15 minutes at 37°C and 1,400 rpm in the
thermoshaker
from CMP-Neu-5-Ac
and Lac-β−OMU per minute at 37 °C at pH 8.0
1 unit
Figure 8. Enzymatic-catalyzed glycosylation using β(1→4)Galactosyltransferase [β(1→4)
GalT] and UDP-Gal as donor substrate.
O
al. (2008). Utilizing flow cytometry to monitor autophagy in living mammalian
cells. Autophagy; 4(5): 621-8.
2. Zhang, L., et al. (2007). Small molecule regulators of autophagy identified by an image-based
al. (2008). Utilizing flow cytometry to monitor autophagy in living mammalian
cells. Autophagy; 4(5): 621-8.
2. Zhang, L., et al. (2007). Small molecule regulators of autophagy identified by an image-based
al. (2008). Utilizing flow cytometry to monitor autophagy in living mammalian
cells. Autophagy; 4(5): 621-8.
2. Zhang, L., et al. (2007). Small molecule regulators of autophagy identified by an image-based
(2008). Utilizing flow cytometry to monitor autophagy in living mammalian
cells. Autophagy; 4(5): 621-8.
2. Zhang, L., et al. (2007). Small molecule regulators of autophagy identified by an image-based
al. (2008). Utilizing flow cytometry to monitor autophagy in living mammalian
cells. Autophagy; 4(5): 621-8.
2. Zhang, L., et al. (2007). Small molecule regulators of autophagy identified by an image-based