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3
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to each well
8. Mix the samples thoroughly by pipetting up and down.
9. Incubate the samples for 30 minutes at room temperature (18 to 25ºC) in the dark.
10. Pipette 80 µL of 1X Assay Buffer BA directly
The hydantoin BW 245C (Prod. No. B 9305)
has been widely used as a selective DP agonist [18-20]; it
may exist as 9-oxo,11-oxo and 9,11-dioxo tautomers.
Inversion of all chiral centers in the ring systems
220201 61�.9 6-10 1 g
5 g
10 g
25 g
References:
1. Bjellqvist, B., et al. 1982. J. Biochem. Biophys.
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. Res. 1, 30–41.
21. Pollack, S. R., Petrov, N., Brankov, G. & Blagoeva, R. (1984) J. Biomech. 17, 627–636.
22. Jacobs, C. R., Yellowley, C. E., Davis, B. R., Zhou, Z., Cimbala, J. M. & Donahue, H. J. (
active 14-654 3 12-550
EGFR, active 14-531 3
EGFR (L858R), active 14-626 3
EGFR (L861Q), active 14-627 3
EGFR (T790M), active 14-725 3
EGFR (T790M, L858R),
active
14-721 3
EphA1, active 14-653 3
/mm/115333
18 LC-MS Contaminants │ Sample preparation is crucial for minimizing contamination
Hypergrade and gradient grade solvents minimize
contaminant peaks
Figure 9 illustrates the influence
et al.
Electroanalysis. �00�, 14(9), 627. (7) Rubio Retama, J. et al. Biosens. Bioelectron.
�004, 20, 1111. (8) Brahim, S. et al. Microchimica Acta �00�, 143, 123. (9)
Abraham, S et al. Biomaterials