mouse
clone CB-64, purified from hybridoma cell culture
Catalog Number SAB4200631
Product Description
Monoclonal Anti-CYBA (mouse IgM isotype) is derived
from the hybridoma CB-64 produced by the
7. Flow Cytometry: First Principles, Givan, A. L.,
Wiley-Liss (New York, NY: 1992), p. 64.
JLH/RXR 12/02
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants
information regarding hazards and safe
handling practices.
Product Profile
A minimum titer of 1:64 was determined by Fluorescent
Dot Immunobinding Assay (FDIBA) on a 5 µg dot of
monkey IgG bound to
.5 mg/ml (prior to the addition of BSA).
A567/A280: 1.5-5.0
A minimum working dilution of 1:64 was determined by
direct immunofluorescent labeling of human peripheral
blood lymphocytes.
In
safe
handling practices.
Product Profile
The minimum working dilution was determined to be 1:64
by direct immunofluorescent labeling of mouse spleen
cells.
In order to obtain best results it
Profile
Fluorescent dot immunobinding assay (F-DIBA):
minimum working dilution on a 4-8 μg dot of rabbit IgG
is 1:64.
Particle Immunofluorescence Assay (P-IFMA):
minimum working dilution on a 50 μL
identification
of in vivo substrates of the ubiquitin pathway. Anal.
Biochem., 282(1), 54-64 (2000).
JBB/MAM-2 10/02
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
and B Cells.
in Immunology Series No. 8 Procedural Guide,
(May 1978), pp. 11-20, U.S. Dept. HEW-PHS, CDC
Bureau of Laboratories, Atlanta, GA 30333.
ADM,MAM 02/11-1
Sigma brand products are sold