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The Effective Use of Protein Kinase Inhibitors
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86 101 105 106 34 37 57 87 81
Product Information Sheet-e64-ro
4. Supplementary Information
y Version: 08E-64, Protease Inhibitor N-[N-(L-3-Trans-carboxirane-2-carbonyl)-L-leucyl]-agmatine
sigma-aldrich.com 7
4. Supplementary Information
4.1. Conventions
The Effective Use of Protein Kinase Inhibitors
www.sigma-aldrich.com/cellsignaling
84 99 99 9 64 59 20 90 93 80 94 103 96 52 55 89 82 89 99 94 102 95
86 101 105 106 34 37 57 87 81 26
Anti-Calpain-94 (LP828588 90 Isoforms) (Domain I) Large Subunit antibody produced in rabbit (C3739)
the LP 82, 85, 88, and 90 isoforms.2 The lens
contains isoforms LP82 and LP85. The latent large
subunit is 94 kDa, and the amino-terminal truncation at
activation yields approximately 64-62 kDa isoforms
Complement C8 (C3535)
mass of 150 kDa. It has three
nonidentical polypeptide chains:
1-3
-chain (64 kDa)
-chain (64 kDa)
-chain (22 kDa)
Complement C8 is present in normal plasma at
Anti-Calpain-94 (Domain II) Large Submit antibody produced in rabbit (C3364)
Subunit detects all
isoforms of human calpain 94, including the LP82, 85,
88, and 90 forms. The antibody also detects isoforms
82, 85, 88, and 90 of rat calpain 94. The antibody
recognizes latent and active
Analysis of Antibiotic Sulfa Drugs Using Ascentis C18
, sulfamerazine, 127-79-7, S8876, sulfamethizole, 144-82-1, S5632, 581324-U
Conditions
column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
mobile phase: 85:15, water with 1% acetic
Product Information Sheet-11836153001
incubation
with the protease
Pancreas extract 0.02 7.8 85 96
Pronase 0.005 7.8 92 98
Thermolysin 0.0005 7.8 98 98
Chymotrypsin 0.0015 7.8 85 93
0.003 7.8 90 93
Papain 0.33 7.8 66 77
The inhibitory
Data Sheet - P8208
storage, clarify
the solution by centrifugation before use.
References
1. Cantley, L., et al., Cell, 64, 281 (1991).
2. Otsu, M., et al., Cell, 65, 91 (1991).
3. Panayotou, C., and Waterfield, M., BioEssays
LABORATORY NOTEBOOK ISSUANCE PAGE
.22 85 0.22
42 2.5 1,870 0.007 0.20 100 0.40
44 16 995 0.007 0.18 80 0.29
50 2.7 2,685 0.015 0.12 97 6
52 7 235 0.015 0.18 96 7
54 20–25 39 0.015
Lab Notebook Guidelines
.22 85 0.22
42 2.5 1,870 0.007 0.20 100 0.40
44 16 995 0.007 0.18 80 0.29
50 2.7 2,685 0.015 0.12 97 6
52 7 235 0.015 0.18 96 7
54 20–25 39 0.015
LABORATORY NOTEBOOK ISSUANCE PAGE
.22 85 0.22
42 2.5 1,870 0.007 0.20 100 0.40
44 16 995 0.007 0.18 80 0.29
50 2.7 2,685 0.015 0.12 97 6
52 7 235 0.015 0.18 96 7
54 20–25 39 0.015
LABORATORY NOTEBOOK ISSUANCE PAGE
.22 85 0.22
42 2.5 1,870 0.007 0.20 100 0.40
44 16 995 0.007 0.18 80 0.29
50 2.7 2,685 0.015 0.12 97 6
52 7 235 0.015 0.18 96 7
54 20–25 39 0.015
Product Information Sheet - R5125
Product Information
Ribonuclease A from bovine pancreas
Sigma Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein
R5125
Product Description
CAS Registry Number
Product Information Sheet - C6143
solution. J. Pharm. Pharmacol., 32(7),
460-462 (1980).
9. Shaker, L.A., et al., Mechanism of resistance of
Bacillus subtilis spores to chlorhexidine. J. Appl.
Bacteriol., 64(6), 531-539 (1988).
IRB
DataSheet - P8340
Pittsburgh, M.S. thesis, p. 7 (2020).
6. Crosland, Rachel, "Studies of The PTEN Tumour
Suppressor in Endometrial Cancer". Sheffield
Hallam University, Ph.D. dissertation, p. 85
(2004).
Data Sheet - SAB4200728
., 3, R45-54
(2014).
5. Di Comite G., et al., J Leukoc Biol., 85, 81-7
(2009).
6. Baudin E., et al., Br J Cancer, 78, 1102-7
(1998).
SG,DR_OKF/LV,PHC 04/17-1
Panorama Ab Microarray - Gene Regulation I List of Antibodies
clone: AH3-120 H0913
35 Acetyl&Phospho Histone H3 (pSer10, Ac-Lys9) clone: APH3-64 H0788
36 Acetyl-Histone H3 ( Ac-Lys9) H9286
37 Acetyl&Phospho Histone H3 (pSer10, Ac-Lys9) H9161
Data Sheet - P3618
al., Cancer Res., 50, 748 (1990).
6. Rubin, K., et al., Lancet, 1, 1353 (1988).
7. Reuterdahl, C., et al., Lab. Invest., 64, 321 (1991).
8. Raines, E.W., et al., Methods Enzymol., 109, 749
(1985).
Data Sheet - C9354
6. Williams, C., et al., Ann. N. Y. Acad. Sci., 889, 72
(1999).
7. Dubois, R. N., Aliment Pharmacol. Ther., 14, Suppl.
1, 64 (2000).
8. Langenbach, R., et al., Cell, 83, 483 (1995).
Data Sheet - P0089
Cell Tissue Res., 314,
85-92 (2003).
5. Mäkinen, T. et al., Cell. Mol. Life Sci., 64, 1915-
1929 (2007).
6. Takahashi, M. et al., Neoplasia, 8, 1003-1010
(2006).
7. Shimoda, M. et al., Clin
Product Information sheet - D9154
through novel
enzyme immobilization techniques". Washington
State University, M.S. thesis, p. 64 (2006).
12. Ryu, Moon-Shun, "Zinc transporter expression in
mature red blood cells and differentiating
Data Sheet - SAB4200833
22-7
(2016).
2. Pilo, P., Front. Cell Infect. Microbiol., 8, 258
(2018).
3. McLendon, M.K. et al., Annu. Rev. Microbiol.,
60, 167-85 (2006).
4. Ancuta, P. et al., Infect. Immun., 64, 2041
59505 Galactosyltransferase Kit
dimethylsulfoxide 72 74 75
methanol 77 80 84
ethanol 80 85 86
acetone 76 76 67
dioxane 72 67 25
acetonitrile 69 66 55
N,N-dimethylformamide 64 55 39
Anti-Calpain-94 (Catalytic Domain) Large Subunit antibody produced in rabbit (C3239)
latent large subunit is 94 kDa, and the amino-
terminal truncation at activation yields approximately
64-62 kDa isoforms. Also, a cascade of smaller forms
can be seen with further activation. Unlike m-calpain
Data Sheet - I0156
Transpl. Immol., 4, 81-85
(1996).
2. Zheng, X., et al., J. Immunol., 154, 5590-5600
(1995).
3. Park, L., et al., J. Exp. Med., 166, 476-488 (1987).
4. Plaut, M., et al., Nature, 339, 64-67 (1989).
5. Kikutani
Data Sheet - I0281
Transpl. Immol., 4, 81-85
(1996).
2. Zheng, X., et al., J. Immunol., 154, 5590-5600
(1995).
3. Park, L., et al., J. Exp. Med., 166, 476-488 (1987).
4. Plaut, M., et al., Nature, 339, 64-67 (1989).
5. Kikutani
Product Information Sheet - P6056
of Proteins and
Peptides, Volume 9, Elsevier, (New York, NY:
1989) p 73.
7. Welinder, K.G., Anal. Biochem., 174, 54-64, (1988).
Related Products
SIGMA HPLC Purified Products:
Product Catalog
Data Sheet - R1281 - Lot 061M0761
Lot Number 061M0761:
>95% (densitometry)
Figure 2.
Specific Activity of Lot Number 061M0761:
64 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5
Data Sheet - F0427
Biol., 45, 657-666 (2000).
7. Hu, C. L., Apoptosis of bovine granulosa cells after
serum withdrawal is mediated by Fas antigen
(CD95) and Fas ligand. Biol. Reprod., 64, 518-526
(2001).
8. Seino
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