# Expected Range Units
NGF Control I HADK2-111 74 - 154 pg/mL
Control II HADK2-211 317 - 659 pg/mL
IL-6 Control I HADK2-111 102 - 212 pg/mL
Control II HADK2-211
produced in rabbit using as immunogen
a synthetic peptide corresponding to amino acid
residues 644-659 of rat PMP70 with N-terminal added
cysteine, conjugated to KLH. The corresponding
sequence is identical
Biochem., 162(1), 296-300 (1987).
3. Metz-Boutigue, M. H., et al., Human
lactotransferrin: amino acid sequence and
structural comparisons with other transferrins. Eur.
J. Biochem., 145(3), 659-676 (1984
produced in rabbit using as immunogen
a synthetic peptide corresponding to amino acid
residues 644-659 of rat PMP70 (Gene ID: 25270),
conjugated to KLH. The corresponding sequence is
identical in mouse
their role in AMP binding. Biochem. J., 346,
659-669 (2000).
Precisio is a trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich Co.
BKR,MAM 10/09-1
Sigma brand products are sold through
amount of enzyme (mg)
References
1. Anest, V. et al., A nucleosomal function for I-kappa-
B kinase-alpha in NF-kappa-B-dependent gene
expression. Nature, 423, 659-663 (2003).
2. Hoshino, K. et al
solution.
References
1. Metz-Boutigue, M. H., et al., Human
lactotransferrin: amino acid sequence and
structural comparisons with other transferrins. Eur.
J. Biochem., 145(3), 659-676 (1984).
2.
and their role in AMP binding. Biochem. J., 346,
659-669 (2000).
PRECISIO is a registered trademark of Sigma-Aldrich
Co. LLC.
JR,SV,MAM 04/12-1
2012 Sigma-Aldrich Co. LLC. All rights reserved
plasminogen activator activity in
human synovial fibroblasts. Biochem. Biophys.
Res.Commun. 180, 652-659 (1991).
6. Kitamura, T., et al., Establishment and
characterisation of a unique human cell line
developed in rabbit using a synthetic peptide
(C)KQIGSLESKLEHLTAS corresponding to amino acid
residues 659–674 of human KCa2.3 as the immunogen.
This sequence has 100% homology with mouse, pig,
and rat. The
be used within
24 hours.
References
1. Bergy, M. E., and Eble, T. E., The filipin complex.
Biochemistry, 7(2), 653-659 (1968).
2. The Merck Index, 13th
com-
mercially available kits. n
References
1. Wilfinger WW et al. (1997) BioTechniques 22:474–481
2. okamoto T, okabe S (2000) int J mol med 6:657–659
3. Fleige S, Pfaffl mW (2006) mol aspects med 27
Recommended procedure for mammalian cells
For 70-90% confluent 100 mm dish (106-107 cells), use 1 ml lysis buffer (50 mM Tris HCl, pH 7.4,
150 mM NaCl, 1 mM EDTA, 1% Triton X-100). If the expression level
PPP1CA P NP_001008709.1 y n/d n/d
609 Protein Phosphatase 1a P7607 5499 PPP1CA M NP_001008709.1 y y y
659 Serine/Threonine Protein Phosphatase 1g1 P7609 5501 PPP1CC P NP_002701.1 y y y
610 Protein Phosphatase
PPP1CA P NP_001008709.1 y n/d n/d
609 Protein Phosphatase 1a P7607 5499 PPP1CA M NP_001008709.1 y y y
659 Serine/Threonine Protein Phosphatase 1g1 P7609 5501 PPP1CC P NP_002701.1 y y y
610 Protein Phosphatase