Buffer. Incubate cells for 20 minutes on ice.
• Remove fixation buffer by centrifugation at 670 x g for 5 minutes. Aspirate and discard
supernatant, only saving the cell pellet.
• For every
Refer to Figure 2 for an example of an experiment performed in
96-well format.
Table 2.
Physical properties of
fluorophores conjugated to
SmartFlare™ RNA Detection
Probes
Figure 2.
Sample map
cells are between 2
x105 to 1 x 106 cells/mL.
13. Transfer 2 x 104 to 1 x 105 cells in 100 µL of serum- or albumin-containing medium (2 x105 to 1 x
106 cells/mL) to
Chemiluminescent
Catalog # 70-600
Sufficient reagents for 96 reactions.
Contents Page
I. INTRODUCTION 2
II. TEST PRINCIPLE 2
III. KIT COMPONENTS 3
IV. SAMPLE PREPARATION 4
V. ASSAY DEVELOPMENT
Colorimetric
Catalog # 70-500
Sufficient reagents for 96 reactions.
Contents Page
I. INTRODUCTION 2
II. TEST PRINCIPLE 2
III. KIT COMPONENTS 3
IV. SAMPLE PREPARATION 4
V. ASSAY DEVELOPMENT
expressed on the cell membrane. Annexin V, CF647 is excitable by a red laser, fluoresces
maximally at 670 nm and can be detected in the Red2 channel of dual laser cytometers. Live cells
demonstrate little
mammalian cells (up to 2 mM for Hep2 cells).
Purity: ≥97% by HPLC. M.W. 283.3
NO O
CO2H
Cat. No. 677520 25 mg € 83
Ref.: Hung, D .T ., et al . 2005 . Science 310, 670
for IL-18. In a 96-well
dilution plate, prepare a serial 1:2 (16 points) or 1:4
(8 points) dilution of human IL-18 (starting at
2,000 ng/mL) in complete medium.
2. In a 96-well flat-bottom
Z., et al. 2000. Nat. Cell Biol. 2, 463.
Haass, C., and De Stooper, B. 1999. Science 286,
916.
Sinha, S., and Lieberburg, I. 1999. Proc. Natl. Acad.
Sci. USA 96, 11049.
Chen, M. 1997. FEBS Lett
microwells of the 96-well clear plate. Can be used with
human, mouse, and rat samples. Sensitivity: 1.6 Units/mL;
Range of Detection: 1.6 to 100 Units/mL.
L6 cells were stained with 2 µg/mL of Anti-GSK3
PEST P9109
622 Negative Control NA
660 Serine/Threonine Protein Phosphatase 2 A/Bg P5359
665 SH-PTP2 (SHP-2) S3056
670 SIRPa1 (SHPS-1) S1311
684 Sos1 S2937
704 Negative Control NA
XP725
PEST P9109
622 Negative Control NA
660 Serine/Threonine Protein Phosphatase 2 A/Bg P5359
665 SH-PTP2 (SHP-2) S3056
670 SIRPa1 (SHPS-1) S1311
684 Sos1 S2937
704 Negative Control NA
XP725
PEST P9109
622 Negative Control NA
660 Serine/Threonine Protein Phosphatase 2 A/Bg P5359
665 SH-PTP2 (SHP-2) S3056
670 SIRPa1 (SHPS-1) S1311
684 Sos1 S2937
704 Negative Control NA
XP725
mutation, “Swedish mutation,” results from the
replacement of Lys and Met by Asn and Leu at
codons 670 and 671. The Swedish mutation does
not lie within Aβ peptide region but lies in the
proximity of the
Sin3A, N-terminal S4445 P y y y
668 Sin3A, C-Terminal S6695 P y n/d n/d
669 Sir2S S5313 P n/d y n/d
670 SIRPα1 (SHPS-1) S1311 P n/d y y
671 Sirt1 S5196 M n/d y n/d
672 Anti Cy3+Cy5 C0992 M NA NA
96
Water Baths Overview
TW Series SW Series
Please refer to the fold-out page for description of icons above.
Water Baths
+20 °C ... +99.9 °C
4 different models
from 2 to 26 liters
035508.2,NP_056292.1,NP_001102231.1 P y n/d n/d
669 Sir2 S5313 93759 Sirt1 NP_062786.1 P n/d y n/d
670 SIRPα1 (SHPS-1) S1311 P n/d y y
671 Sirt1 S5196 93759 Sirt1 NP_062786.1 M n/d y n/d
672 Anti Cy3+Cy5
035508.2,NP_056292.1,NP_001102231.1 P y n/d n/d
669 Sir2 S5313 93759 Sirt1 NP_062786.1 P n/d y n/d
670 SIRPα1 (SHPS-1) S1311 P n/d y y
671 Sirt1 S5196 93759 Sirt1 NP_062786.1 M n/d y n/d
672 Anti Cy3+Cy5