Homozygous G
Sample B1
Homozygous T
Sample D1
Heterozygous G/T
0
100
200
300
400
500
600
700
1/4 1/8 1/16 1/32
Sequencing Reaction Size
Q
ua
lit
y
S
co
re
Phred-20 Phred-30 Phred-40
fragments have 5′-protruding ends and can be labeled with radioactive nucleotides, such as [32 P]-dTTP or
[32 P]-dGTP by standard filling-in reactions.
• End-labeling reactions can be performed with
MultiScreen filter plate with Ultracel-10 membrane can be used with conical
100 µL, standard 300 µL or 700 µL well microtiter deep well receiver plates.
Materials Required
The MultiScreen filter
to pellet
the microspheres.
16. Repeat steps 13 to 15 twice.
17. suspend the microspheres in 700 μL of activation/
coupling buffer (See antibody coupling section). It is
important that the microspheres