70221

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Immunohistochemical characterization of novel monoclonal antibodies against the N-terminus of amyloid β-peptide.

Nicolaas A Verwey et al.

Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis, 20(3), 179-187 (2013-07-09)

Abstract Amyloid β-peptide (Aβ) is a key molecule in Alzheimer's disease (AD). Reliable immunohistochemical (IHC) methods to detect Aβ and Aβ-associated factors (AAF) in brain specimens are needed to determine their role in AD pathophysiology. Formic acid (FA) pre-treatment, which

Direct analysis of formate in human plasma, serum and whole blood by in-line coupling of microdialysis to capillary electrophoresis for rapid diagnosis of methanol poisoning.

Pavel Kubáň et al.

Analytica chimica acta, 768, 82-89 (2013-03-12)

A simple method using direct injection of human blood samples and quantitative analysis of formate was developed for rapid diagnosis of methanol poisoning. A sample pretreatment device including a 500Da molecular weight cut-off dialysis membrane was in-line coupled to capillary

Evaluation of ¹³C- and ²H-labeled internal standards for the determination of amphetamines in biological samples, by reversed-phase ultra-high performance liquid chromatography-tandem mass spectrometry.

Thomas Berg et al.

Journal of chromatography. A, 1344, 83-90 (2014-05-02)

Stable isotope-labeled internal standards (SIL-ISs) are often used when applying liquid chromatography-tandem mass spectrometry (LC-MS/MS) to analyze for legal and illegal drugs. ISs labeled with (13)C, (15)N, and (18)O are expected to behave more closely to their corresponding unlabeled analytes

Development and validation of a LC-MS/MS assay for quantitation of plasma citrulline for application to animal models of the acute radiation syndrome across multiple species.

Jace W Jones et al.

Analytical and bioanalytical chemistry, 406(19), 4663-4675 (2014-05-21)

The potential risk of a radiological catastrophe highlights the need for identifying and validating potential biomarkers that accurately predict radiation-induced organ damage. A key target organ that is acutely sensitive to the effects of irradiation is the gastrointestinal (GI) tract

Development of a stability-indicating HPLC method of etifoxine with characterization of degradation products by LC-MS/TOF, 1H and 13C NMR.

Nadia Djabrouhou et al.

Journal of pharmaceutical and biomedical analysis, 100, 11-20 (2014-08-15)

This paper describes a new LC-MS/TOF method for the degradation products determination when Etifoxine (ETI) is submitted to different stress conditions. Chromatography is performed by using Kromasil C18 column (250mm×4.6mm, 5μm particle size). The selected mobile phase consists of formate

Stability of extemporaneously compounded dexamethasone in glass and plastic bottles and plastic syringes.

Mary H H Ensom et al.

The Canadian journal of hospital pharmacy, 67(4), 274-279 (2014-09-13)

Dexamethasone is widely used to treat rheumatic and endocrine disorders and chemotherapy-induced nausea and vomiting. A palatable, alcohol-free liquid formulation, with a suitable concentration to allow reasonable administration volume, is available only via extemporaneous compounding. To evaluate the stability of

In vitro assessment of time-dependent inhibitory effects on CYP2C8 and CYP3A activity by fourteen protein kinase inhibitors.

Anne M Filppula et al.

Drug metabolism and disposition: the biological fate of chemicals, 42(7), 1202-1209 (2014-04-10)

Previous studies have shown that several protein kinase inhibitors are time-dependent inhibitors of cytochrome P450 (CYP) 3A. We screened 14 kinase inhibitors for time-dependent inhibition of CYP2C8 and CYP3A. Amodiaquine N-deethylation and midazolam 1'-hydroxylation were used as marker reactions for

Mass spectrometric quantification of glucosylsphingosine in plasma and urine of type 1 Gaucher patients using an isotope standard.

Mina Mirzaian et al.

Blood cells, molecules & diseases, 54(4), 307-314 (2015-04-07)

Deficiency of glucocerebrosidase (GBA) leads to Gaucher disease (GD), an inherited disorder characterised by storage of glucosylceramide (GlcCer) in lysosomes of tissue macrophages. Recently, we reported marked increases of deacylated GlcCer, named glucosylsphingosine (GlcSph), in plasma of GD patients. To

Evaluation of enantiomeric purity of magnesium-L-aspartate dihydrate.

Oliver Wahl et al.

Journal of pharmaceutical and biomedical analysis, 102, 100-109 (2014-09-30)

Magnesium supplementation in form of organic magnesium salts is a very popular practice. We examined the enantiomeric purity of "Magnesium aspartate dihydrate" monographed in the European Pharmacopeia. A chiral capillary zone electrophoresis using (2-hydroxypropyl)-β-cyclodextrin coupled to laser induced fluorescence detection

An application of target profiling analyses in the hepatotoxicity assessment of herbal medicines: comparative characteristic fingerprint and bile acid profiling of Senecio vulgaris L. and Senecio scandens Buch.-Ham.

Aizhen Xiong et al.

Analytical and bioanalytical chemistry, 406(29), 7715-7727 (2014-09-23)

The toxicity assessment of herbal medicines is important for human health and appropriate utilization of these medicines. However, challenges have to be overcome because of the complexity of coexisting multiple components in herbal medicines and the highly interconnected organismal system.

Searching the cytochrome p450 enzymes for the metabolism of meranzin hydrate: a prospective antidepressant originating from Chaihu-Shugan-San.

Xi Huang et al.

PloS one, 9(11), e113819-e113819 (2014-11-27)

Meranzin hydrate (MH), an absorbed bioactive compound from the Traditional Chinese Medicine (TCM) Chaihu-Shugan-San (CSS), was first isolated in our laboratory and was found to possess anti-depression activity. However, the role of cytochrome P450s (CYPs) in the metabolism of MH

Evaluation of a LC-MS method for everolimus preclinical determination in brain by using [(13)C2D4]RAD001 internal standard.

Stefano Giovagnoli et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 985, 155-163 (2015-02-16)

Isotopic internal standards are increasingly frequent in LC-MS analysis to control biological matrix effects in the quantitation of immunosuppressant drugs, such as everolimus (RAD001). Here we present the evaluation of a LC-MS method, exploiting [(13)C2D4]RAD001 as internal standard, for preclinical

Effects of dietary supplementation with two potassium formate sources on performance of 8- to 22-kg pigs.

J K Htoo et al.

Journal of animal science, 90 Suppl 4, 346-349 (2013-02-13)

Dietary inclusion of salts of organic acids may modulate intestinal microbiota and enhance performance of pigs. Published data on the effects of potassium formate (KF; pH 6.0-8.5) on pig performance are scarce compared with that of potassium diformate (KDF; pH

Screening of drugs and toxic compounds with liquid chromatography-linear ion trap tandem mass spectrometry.

François-Ludovic Sauvage et al.

Clinical chemistry, 52(9), 1735-1742 (2006-07-22)

In clinical and forensic toxicology, general unknown screening is used to detect and identify exogenous compounds. In this study, we aimed to develop a comprehensive general unknown screening method based on liquid chromatography coupled with a hybrid triple-quadrupole linear ion

Comparison of capillary electrophoresis-mass spectrometry and hydrophilic interaction chromatography-mass spectrometry for anionic metabolic profiling of urine.

Miranda G M Kok et al.

Talanta, 132, 1-7 (2014-12-06)

In order to assess the utility of a recently developed capillary electrophoresis-mass spectrometry (CE-MS) method for the study of anionic metabolites in urine, a comparison was made with hydrophilic interaction chromatography-MS (HILIC-MS) using negative electrospray ionization. After optimization of the

Development and Validation of a LC-MS/MS Method for the Simultaneous Estimation of Amlodipine and Valsartan in Human Plasma: Application to a Bioequivalence Study.

Hemanth Jangala et al.

Scientia pharmaceutica, 82(3), 585-600 (2015-04-09)

A reliable, simple, and robust liquid chromatography-tandem mass spectro-metric (LC-MS/MS) method has been developed and validated that employs solid-phase extraction for the simultaneous estimation of amlodipine and valsartan in human K3EDTA plasma using amlodipine-d4 and valsartan-d9 as internal standards. Chromatographic

A high-performance liquid chromatography-tandem mass spectrometry method coupled with protein precipitation for determination of granisetron in human plasma and its application to a comparative pharmacokinetic study.

Ying Zhou et al.

Biomedical chromatography : BMC, 28(12), 1597-1600 (2014-05-09)

A rapid, simple and validated method based on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has been developed for the determination of granisetron in human plasma. Plasma samples were pre-purified by protein precipitation procedure. The chromatographic separation was achieved

Detection and characterization of prednisolone metabolites in human urine by LC-MS/MS.

Xavier Matabosch et al.

Journal of mass spectrometry : JMS, 50(3), 633-642 (2015-03-25)

Glucocorticosteroids are prohibited in sports when used by systemic administrations (e.g. oral), whereas they are allowed using other administration ways. Strategies to discriminate between administrations routes have to be developed by doping control laboratories. For this reason, the metabolism of

Determination of bisphenol A, triclosan and their metabolites in human urine using isotope-dilution liquid chromatography-tandem mass spectrometry.

Gilles Provencher et al.

Journal of chromatography. A, 1348, 97-104 (2014-05-20)

Bisphenol A (BPA) and triclosan (TCS) are ubiquitous environmental phenols exhibiting endocrine disrupting activities that may be involved in various health disorders in humans. There is a need to measure separately free forms and conjugated metabolites because only the former

A novel quantification method for analysis of twenty natural amino acids in human serum based on N-phosphorylation labeling using reversed-phase liquid chromatography-tandem mass spectrometry.

Xiaowu Chen et al.

Analytica chimica acta, 836, 61-71 (2014-07-01)

A novel method based on the strategy of N-phosphorylation labeling is described for quantification of twenty natural amino acids in human serum by reversed-phase liquid chromatography-electrospray tandem mass spectrometry (RP-LC/ESI-MS). The derivatization reaction was easily performed in one-pot reaction under

Evolution of strategies to achieve baseline separation of ten anionic, water-soluble sulfated estrogens via achiral packed column supercritical fluid chromatography.

M A Patel et al.

Journal of chromatography. A, 1370, 240-245 (2014-12-03)

Near baseline separation of ten sulfated sodium salts of various structurally related estrogens employing a variety of bonded stationary phase packed columns was obtained using a conventional supercritical fluid chromatograph coupled with UV detection. Critical pairs 2/3 (8,9-dehydroestrone/17β-dihydroequilin) and 6/7

An improved liquid chromatography-tandem mass spectrometric method to quantify formoterol in human urine.

Genye He et al.

Journal of chromatographic science, 52(8), 848-851 (2013-09-14)

Formoterol is a new threshold substance in the prohibited list 2012 according to World Anti-Doping Agency. Extracted by ethyl acetate using formoterol-D6 as internal standard, formoterol underwent a constant flow rate gradient elution separation in reversed-phase liquid chromatography. Subsequently, mass

Nontargeted metabolite profiling discriminates diet-specific biomarkers for consumption of whole grains, fatty fish, and bilberries in a randomized controlled trial.

Kati Hanhineva et al.

The Journal of nutrition, 145(1), 7-17 (2014-12-21)

Nontargeted metabolite profiling allows for concomitant examination of a wide range of metabolite species, elucidating the metabolic alterations caused by dietary interventions. The aim of the current study was to investigate the effects of dietary modifications on the basis of

High-throughput tandem mass spectrometry multiplex analysis for newborn urinary screening of creatine synthesis and transport disorders, Triple H syndrome and OTC deficiency.

Christiane Auray-Blais et al.

Clinica chimica acta; international journal of clinical chemistry, 436, 249-255 (2014-06-10)

Creatine synthesis and transport disorders, Triple H syndrome and ornithine transcarbamylase deficiency are treatable inborn errors of metabolism. Early screening of patients was found to be beneficial. Mass spectrometry analysis of specific urinary biomarkers might lead to early detection and

Identification of CYP3A7 for glyburide metabolism in human fetal livers.

Diana L Shuster et al.

Biochemical pharmacology, 92(4), 690-700 (2014-12-03)

Glyburide is commonly prescribed for the treatment of gestational diabetes mellitus; however, fetal exposure to glyburide is not well understood and may have short- and long-term consequences for the health of the child. Glyburide can cross the placenta; fetal concentrations

Monoclonal antibodies selective for α-synuclein oligomers/protofibrils recognize brain pathology in Lewy body disorders and α-synuclein transgenic mice with the disease-causing A30P mutation.

Therese Fagerqvist et al.

Journal of neurochemistry, 126(1), 131-144 (2013-02-01)

Inclusions of intraneuronal alpha-synuclein (α-synuclein) can be detected in brains of patients with Parkinson's disease and dementia with Lewy bodies. The aggregation of α-synuclein is a central feature of the disease pathogenesis. Among the different α-synuclein species, large oligomers/protofibrils have

Mechanistic understanding of brain drug disposition to optimize the selection of potential neurotherapeutics in drug discovery.

Irena Loryan et al.

Pharmaceutical research, 31(8), 2203-2219 (2014-03-14)

The current project was undertaken with the aim to propose and test an in-depth integrative analysis of neuropharmacokinetic (neuroPK) properties of new chemical entities (NCEs), thereby optimizing the routine of evaluation and selection of novel neurotherapeutics. Forty compounds covering a

Evaluation of mobile phase characteristics on three zwitterionic columns in hydrophilic interaction liquid chromatography mode for liquid chromatography-high resolution mass spectrometry based untargeted metabolite profiling of Leishmania parasites.

Rong Zhang et al.

Journal of chromatography. A, 1362, 168-179 (2014-08-28)

It has been reported that HILIC column chemistry has a great effect on the number of detected metabolites in LC-HRMS-based untargeted metabolite profiling studies. However, no systematic investigation has been carried out with regard to the optimisation of mobile phase

Capillary electrophoresis with contactless conductometric detection for rapid screening of formate in blood serum after methanol intoxication.

Petr Kubáň et al.

Journal of chromatography. A, 1281, 142-147 (2013-02-20)

A new method for rapid, direct determination of formate in blood serum samples by capillary electrophoresis with contactless conductometric detection is presented. A selective separation of formate was achieved in approximately 1 min using an electrolyte system comprising 10 mM

Potent anti-proliferative, pro-apoptotic activity of the Maytenus royleanus extract against prostate cancer cells: evidence in in-vitro and in-vivo models.

Maria Shabbir et al.

PloS one, 10(3), e0119859-e0119859 (2015-03-24)

Prostate cancer is a leading of cause of cancer related death in men. Despite intensive investment in improving early diagnosis, it often escapes timely detection. Mortality remains high in advanced stage prostate cancer where palliative care remains the only option.

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