RAB0130A: 96-wells
(12 strips x 8 wells) coated with anti-Human ENA-78
• 20X Wash Buffer (Item B)-RABWASH4: 25 mL of 20X concentrated solution
• Lyophilized Human ENA-78 Protein Standard (Item C)-RAB0130C
Reservoir: 8 mm (.31”)
female, quick connect
Distribution Pump Outlet:
20 mm (.78”) hose barb
Inlet to Back Pressure Valve (loop return):
20 mm (.78”) hose barb
Reservoir Drain Valve: 20 mm (.78”)
8. Add 1 mL of ice-cold 1x PBS + 10 M ROCK inhibitor to the organoid pellet.
9. Break the organoids with a P-1000 micropipette by placing pipet tip perpendicular to bottom of tube. Repeat 30 times
Temperature
STOP
Temperature*
Run-Out Window**
+10°C/+50°F +8°C/+46°F 3 days (A), 8 days (B), 14 days
(C)s
+34°C/+93°F +30°C/+86°F 3 hours
* The temperature
204
(1981).
2. Wróblewska, B. et al., Int. J. Food Sci. Tech.,
39(8), 839-850 (2004).
3. Doig, N.M. et al., J. Neurosci., 30(44),
14610-14618 (2010).
4. Szymkiewicz, A., and Chudzik-Kozłowska
reacts in immunoblotting with PKC ζ (78-
80 kDa proteins appearing as doublet) in NIH 3T3
mouse fibroblasts lysate. In rat brain extract the
antiserum reacts with PKC ζ (78 kDa). Degradation
product of 50
at 2-8 C.
Collect the supernatant for assay and
keep on ice.
2. Liquid samples can be tested directly. If
samples will not be assayed immediately,
store at -80 C.
3.
(5 mg Protease) into each
beaker.
8. Cover each beaker with aluminum foil and place in
60 °C water bath. With continuous agitation,
incubate for 30 minutes after the internal
temperature of
204
(1981).
2. Wróblewska, B. et al., Int. J. Food Sci. Tech, 39(8),
839-850 (2004).
3. Doig, N.M. et al. J. Neurosci., 30(44), 14610-14618
(2010).
4. Szymkiewicz, A., and Chudzik-Kozłowska
al. Room-temperature-storable PCR
mixes for SARS-CoV-2 detection. Clinical
biochemistry vol. 84:73-78 (2020).
Notice
We provide information and advice to our customers
on application technologies