For research use only. Not for use in diagnostic procedures.
03-0185-00 Rev 07/23 9
Assay Preparation
Sample Preparation
Prepare samples by one of the following methods
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60
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100
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KBr
∆9-TETRAHYDROCANNABINOL
Methanol Solution
Product Number T 4764
Lot
20442199w Rev 07/20 1 of 3
Intended Use
For single-step purification of genomic DNA from
cultured cells. This protocol has been developed for up
to 1 x 106 human or animal cells.
Storage and
20442199w Rev 07/20 1 of 3
Intended Use
For single-step purification of genomic DNA from
cultured cells. This protocol has been developed for up
to 1 x 106 human or animal cells.
Storage and
20442196w Rev 07/20 1 of 3
Intended Use
For single-step purification of genomic DNA from
tissue samples. This protocol has been developed for
1 – 20 mg human and animal tissue samples. 10 mg
is
20442196w Rev 07/20 1 of 3
Intended Use
For single-step purification of genomic DNA from
tissue samples. This protocol has been developed for
1 – 20 mg human and animal tissue samples. 10 mg
is
20441295w REV 07/20 1 of 3
Intended Use
For purification of genomic DNA from liquid blood
samples. This protocol has been developed for
200 µL to 1,000 µL of human or animal whole blood
(EDTA
20442229w Rev 07/20 1 of 4
Quick-Start Protocol (See Standard Protocol for detailed instructions.)
Lysis
• Add 10 – 50 mg of plant tissue sample per well
of Tube Strips.
• If
20442229w Rev 07/20 1 of 4
Quick-Start Protocol (See Standard Protocol for detailed instructions.)
Lysis
• Add 10 – 50 mg of plant tissue sample per well
of Tube Strips.
• If
8. After incubation at 60 °C, increase the temperature
to 80 °C and incubate for additional 10 minutes
with maximum agitation.
9. After having performed lysis, add 25 µL Clearing
Solution P
due to loss of
solvent.
CMW,MAM 07/16-1
High Pressure Liquid Chromatography
Column: Supelco Discovery C18, 5 m, 2.1 250 mm
Mobile Phase: 80% Methanol/20% Water (18 Mcm)
Gradient
high values. Use no more than 10 IU heparin per mL of blood collected.
HSCR-32K. 07-MAR-2013 PAGE 9 EMD MILLIPORE
PREPARATION OF REAGENTS FOR IMMUNOASSAY
London, 1994).
3. Journal of the American Oil Chemists' Society, 58,
13 (1981).
CMH/NSB 10/07
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants that
For research use only. Not for use in diagnostic procedures.
MCVD1MAG-77K Rev 07/22 9
• Avoid multiple (> 2) freeze/thaw cycles.
• When using frozen samples, it is recommended
BMG 6H8)
For life science research only.
Not for use in diagnostic procedures.
y Version: 07
Content Version: December 2020
Monoclonal antibody to the thymidine-analog 5-bromo-2′-deoxyuridine