Reconstitute according to
label instructions
8000 pg/mL
2 0.25 mL 0.25 mL (1) 4,000
3 0.25 mL 0.25 mL (2) 2,000
4 0.25 mL 0.25 mL (3) 1,000
5 0.25 mL 0.25 mL (4)
Centrifuge 30-60 s at 8000 × g (as above).
� • Discard the flowthrough solution.
• Reconnect the Filter Tube to the same Collection Tube.
• Add 300 �l Wash Buffer.
• Centrifuge 30-60 s at 8000 × g (as above)
4000
6000
8000
10000
a.i.
/D=/data/sara/humanser/051602/Sigma_Cl_serum_10Kfiltrate_C18_64/1SRef/pdata/1 Administrator Tue May 28 16:16:42 2002
1 to 4 ml of serum or plasma
minutes.
15. Centrifuge the samples at 8000 g for 10 minutes.
16. Transfer the supernatant (approximately 650 µL)
into scintillation tubes.
17. Add 4 mL of scintillation liquid.
18. Cap the