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Cong T Trinh et al.
Applied and environmental microbiology, 77(14), 4894-4904 (2011-06-07)
Fermentation enables the production of reduced metabolites, such as the biofuels ethanol and butanol, from fermentable sugars. This work demonstrates a general approach for designing and constructing a production host that uses a heterologous pathway as an obligately fermentative pathway
Jeremy J Minty et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(36), 14592-14597 (2013-08-21)
Synergistic microbial communities are ubiquitous in nature and exhibit appealing features, such as sophisticated metabolic capabilities and robustness. This has inspired fast-growing interest in engineering synthetic microbial consortia for biotechnology development. However, there are relatively few reports of their use
Jingnan Lu et al.
Applied microbiology and biotechnology, 96(1), 283-297 (2012-08-07)
Wild-type Ralstonia eutropha H16 produces polyhydroxybutyrate (PHB) as an intracellular carbon storage material during nutrient stress in the presence of excess carbon. In this study, the excess carbon was redirected in engineered strains from PHB storage to the production of
Michael H Abraham et al.
European journal of medicinal chemistry, 43(3), 478-485 (2007-06-05)
Values of in vitro gas to lung partition coefficients, K(lung), of VOCs have been collected from the literature. For 44 VOCs, application of the Abraham solvation equation to log K(lung) yielded a correlation with R(2)=0.968 and S.D.=0.25 log units. Combination
Fumio Matsuda et al.
Bioscience, biotechnology, and biochemistry, 76(11), 2139-2141 (2012-11-08)
To increase isobutanol production in Saccharomyces cerevisiae, the valine biosynthetic pathway was activated by overexpression of the relevant enzymes in the mitochondria and the cytosol. Native mitochondrial enzymes were overepxressed in the cytosol by deleting the mitochondrial transit peptides. The
Luis H Reyes et al.
Applied and environmental microbiology, 79(17), 5313-5320 (2013-07-03)
Cross-tolerance and antagonistic pleiotropy have been observed between different complex phenotypes in microbial systems. These relationships between adaptive landscapes are important for the design of industrially relevant strains, which are generally subjected to multiple stressors. In our previous work, we
Giulia Caron et al.
Journal of medicinal chemistry, 48(9), 3269-3279 (2005-04-29)
Growing interest in the use of both the logarithm of the partition coefficient of the neutral species in the alkane/water system (log P(N)(alk)) and the difference between log P(N)(oct) (the logarithm of the partition coefficient of the neutral species in
Won-Heong Lee et al.
Bioprocess and biosystems engineering, 35(9), 1467-1475 (2012-05-01)
Engineering of Saccharomyces cerevisiae to produce advanced biofuels such as isobutanol has received much attention because this yeast has a natural capacity to produce higher alcohols. In this study, construction of isobutanol production systems was attempted by overexpression of effective
Konstantinos Grintzalis et al.
Applied and environmental microbiology, 80(18), 5561-5571 (2014-07-09)
We show here that oxidative stress is involved in both sclerotial differentiation (SD) and aflatoxin B1 biosynthesis in Aspergillus flavus. Specifically, we observed that (i) oxidative stress regulates SD, as implied by its inhibition by antioxidant modulators of reactive oxygen
Han Li et al.
Science (New York, N.Y.), 335(6076), 1596-1596 (2012-03-31)
One of the major challenges in using electrical energy is the efficiency in its storage. Current methods, such as chemical batteries, hydraulic pumping, and water splitting, suffer from low energy density or incompatibility with current transportation infrastructure. Here, we report
D Norbäck et al.
Occupational and environmental medicine, 52(6), 388-395 (1995-06-01)
As a part of the worldwide European Community respiratory health survey, possible relations between symptoms of asthma, building characteristics, and indoor concentration of volatile organic compounds (VOCs) in dwellings were studied. The study comprised 88 subjects, aged 20-45 years, from
Cong T Trinh
Applied microbiology and biotechnology, 95(4), 1083-1094 (2012-06-09)
Elementary mode (EM) analysis based on the constraint-based metabolic network modeling was applied to elucidate and compare complex fermentative metabolisms of Escherichia coli for obligate anaerobic production of n-butanol and isobutanol. The result shows that the n-butanol fermentative metabolism was
A novel pathway to produce butanol and isobutanol in Saccharomyces cerevisiae
Branduardi P, et al.
Biotechnology for Biofuels, 6(1), 68-68 (2013)
Jacek Sein Anand et al.
Przeglad lekarski, 69(8), 589-591 (2012-12-19)
We presented the male who was addicted to ethanol, and who drank the mixture of different toxic alcohols. Toxicological screening of ingested fluid found 42% vol propan-1-OL, and 17% vol propan-2-OL (isopropanol). After regaining consciousness, the patient stated that substance
Prasenjit Seal et al.
The Journal of chemical physics, 136(3), 034306-034306 (2012-01-28)
The purpose of the present investigation is to calculate partition functions and thermodynamic quantities, viz., entropy, enthalpy, heat capacity, and Gibbs free energies, for 1-butanol, 2-methyl-1-propanol, and butanal in the vapor phase. We employed the multi-structural (MS) anharmonicity method and
Stéphanie Rollero et al.
Applied microbiology and biotechnology, 99(5), 2291-2304 (2014-11-22)
Volatile compounds produced by yeast during fermentation greatly influence the organoleptic qualities of wine. We developed a model to predict the combined effects of initial nitrogen and phytosterol content and fermentation temperature on the production of volatile compounds. We used
SPR Catalysis V26 (2014)
Shanshan Li et al.
Biotechnology letters, 34(12), 2253-2258 (2012-09-04)
High-level constitutive gene expression can result in cellular metabolic imbalance and limit production. To circumvent these problems, a P(alsSD)-controlled auto-inducible 2-ketoisovalerate biosynthetic pathway and a P(spac)-controlled IPTG-inducible Ehrlich pathway were constructed in Bacillus subtilis to modulate gene expression. Based on
Xiaoqiang Jia et al.
Applied biochemistry and biotechnology, 168(1), 1-9 (2011-05-04)
Isobutanol can be biosynthesized via α-ketoisovalerate catalyzed by heterologous keto acid decarboxylase (KDC) and alcohol dehydrogenase (ADH). In this work, isobutanol biosynthesis pathway was designed in Bacillus subtilis, a notable solvent-tolerant host. In order to do that, a plasmid pPKA
Sensitive analysis of alkyl alcohols as decomposition products of alkyl nitrites in human whole blood and urine by headspace capillary GC with cryogenic oven trapping
Watanabe-Suzuki K, et al.
Journal of Chromatographic Science, 41(2), 63-66 (2003)
Gabriel M Rodriguez et al.
Nature chemical biology, 10(4), 259-265 (2014-03-13)
To expand the capabilities of whole-cell biocatalysis, we have engineered Escherichia coli to produce various esters. The alcohol O-acyltransferase (ATF) class of enzyme uses acyl-CoA units for ester formation. The release of free CoA upon esterification with an alcohol provides
Antonino Baez et al.
Applied microbiology and biotechnology, 90(5), 1681-1690 (2011-05-07)
Promising approaches to produce higher alcohols, e.g., isobutanol, using Escherichia coli have been developed with successful results. Here, we translated the isobutanol process from shake flasks to a 1-L bioreactor in order to characterize three E. coli strains. With in
Ewa Papajak et al.
The Journal of chemical physics, 137(10), 104314-104314 (2012-09-18)
We calculate the standard state entropy, heat capacity, enthalpy, and Gibbs free energy for 13 radicals important for the combustion chemistry of biofuels. These thermochemical quantities are calculated from recently proposed methods for calculating partition functions of complex molecules by
The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading.
Evert K Holwerda et al.
Biotechnology for biofuels, 7(1), 155-155 (2014-11-08)
Clostridium thermocellum is a model thermophilic organism for the production of biofuels from lignocellulosic substrates. The majority of publications studying the physiology of this organism use substrate concentrations of ≤10 g/L. However, industrially relevant concentrations of substrate start at 100
Buyuan Guan et al.
Nanoscale, 4(20), 6588-6596 (2012-09-15)
A general synthetic procedure for highly ordered and well-dispersed periodic mesoporous organosilica (PMO) nanoparticles is reported based on a single cationic surfactant cetyltrimethylammonium bromide (CTAB) and simple silica sources with organic bridging groups via an ammonia-catalyzed sol-gel reaction. By changing
N Inglik et al.
Laboratornoe delo, (8)(8), 24-27 (1989-01-01)
The developed gas-chromatographic vapour-phase analysis of volatile components of biological fluids has been used to study the urine volatile organic compounds (VOC) in normal subjects and patients with diabetes mellitus. The results have permitted the detection of a normal urine
Jan-Karl Guterl et al.
ChemSusChem, 5(11), 2165-2172 (2012-10-23)
The limited supply of fossil resources demands the development of renewable alternatives to petroleum-based products. Here, biobased higher alcohols such as isobutanol are versatile platform molecules for the synthesis of chemical commodities and fuels. Currently, their fermentation-based production is limited
Nobutaka Nakashima et al.
Journal of bioscience and bioengineering, 114(1), 38-44 (2012-05-09)
Sugar derived from biomass is usually a mixture of glucose and other sugars. When mixed sugars are fed to Escherichia coli, glucose is preferentially utilized while other sugars remain unutilized. This phenomenon is known as carbon catabolite repression (CCR). To
Pedram Fatehi
Biotechnology progress, 29(2), 297-310 (2013-01-09)
In this review, the recent advancements and technical challenges associated with the production of ethanol, butanol, and isobutanol via bioconversion routes from celluloses of woody materials are reviewed. Physicochemical processes, e.g. steam explosion, seem to be the most viable process
Malcolm E Tessensohn et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 16(1), 160-168 (2014-11-25)
Voltammetric experiments with 9,10-anthraquinone and 1,4-benzoquinone performed under controlled moisture conditions indicate that the hydrogen-bond strengths of alcohols in aprotic organic solvents can be differentiated by the electrochemical parameter ΔEp (red) =|Ep (red(1)) -Ep (red(2)) |, which is the potential
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