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Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor
Lamichhane R, et al.
Bio-protocol, 7(12) (2017)
Renato M S Cardoso et al.
Biochimica et biophysica acta. Biomembranes, 1862(3), 183157-183157 (2019-12-18)
Association of amphiphiles with biomembranes is important for their availability at specific locations in organisms and cells, being critical for their biological function. A prominent role is usually attributed to the hydrophobic effect, and to electrostatic interactions between charged amphiphiles
Sophia C Goodchild et al.
PloS one, 9(8), e104492-e104492 (2014-08-08)
Although the molecular mechanisms underlying the pathology of amyloidoses are not well understood, the interaction between amyloid proteins and cell membranes is thought to play a role in several amyloid diseases. Amyloid fibrils of β2-microglobulin (β2m), associated with dialysis-related amyloidosis
Julia Skalska et al.
Nanoscale, 11(48), 23366-23381 (2019-12-04)
One of the most important causes of failure in tumour treatment is the development of resistance to therapy. Cancer cells can develop the ability to lose sensitivity to anti-neoplastic drugs during reciprocal crosstalk between cells and their interaction with the
Jonathan F Tait et al.
Analytical biochemistry, 329(1), 112-119 (2004-05-12)
We developed a method for measuring the binding affinity of annexin V for phospholipid vesicles and cells at very low levels of membrane occupancy. The annexin V-117 mutant was labeled with fluorescein iodoacetamide on its single N-terminal cysteine residue; binding
Arnaud Marquette et al.
Biochimica et biophysica acta. Biomembranes, 1862(2), 183149-183149 (2019-12-10)
Viral protein R (Vpr) is a small accessory protein of 96 amino acids that is present in Human and simian immunodeficiency viruses. Among the very different properties that Vpr possesses we can find cell penetrating capabilities. Based on this and
Rajan Lamichhane et al.
Structure (London, England : 1993), 28(3), 371-377 (2020-01-25)
G-protein-coupled receptors (GPCRs) mediate a wide range of human physiological functions by transducing extracellular ligand binding events into intracellular responses. GPCRs can activate parallel, independent signaling pathways mediated by G proteins or β-arrestins. Whereas "balanced" agonists activate both pathways equally
Sumie Manno et al.
The Journal of biological chemistry, 285(44), 33923-33929 (2010-08-21)
Human erythrocytes are continuously exposed to glucose, which reacts with the amino terminus of the β-chain of hemoglobin (Hb) to form glycated Hb, HbA1c, levels of which increase with the age of the circulating cell. In contrast to extensive insights
Saba Ghazvini et al.
Langmuir : the ACS journal of surfaces and colloids, 34(3), 1159-1170 (2017-10-12)
Lipid membranes, a major component of cells, are subjected to significant changes in pH depending on their location in the cell: the outer leaflet of the cell membrane is exposed to a pH of 7.4 whereas lipid membranes that make
Stéphanie Jephthah et al.
Biomolecules, 10(4) (2020-04-23)
Magnesium transporter A (MgtA) is an active transporter responsible for importing magnesium ions into the cytoplasm of prokaryotic cells. This study focuses on the peptide corresponding to the intrinsically disordered N-terminal region of MgtA, referred to as KEIF. Primary-structure and
Alessandra Luchini et al.
Analytical chemistry, 92(1), 1081-1088 (2019-11-27)
In vitro characterization of membrane proteins requires experimental approaches providing mimics of the microenvironment that proteins encounter in native membranes. In this context, supported lipid bilayers provide a suitable platform to investigate membrane proteins by a broad range of surface-sensitive
Irene S Tobias et al.
The Biochemical journal, 473(4), 509-523 (2015-12-05)
Atypical protein kinase C (aPKC) isoenzymes are key modulators of insulin signalling, and their dysfunction correlates with insulin-resistant states in both mice and humans. Despite the engaged interest in the importance of aPKCs to type 2 diabetes, much less is
Peter A Leventis et al.
Annual review of biophysics, 39, 407-427 (2010-03-03)
Phosphatidylserine (PS) is the most abundant negatively charged phospholipid in eukaryotic membranes. PS directs the binding of proteins that bear C2 or gamma-carboxyglutamic domains and contributes to the electrostatic association of polycationic ligands with cellular membranes. Rather than being evenly
Phosphoserine imprinted nanosensor for detection of Cancer Antigen 125
Buyuktiryaki S, et al.
Talanta, 167, 172-180 (2017)
Hongbing Yao et al.
The Journal of biological chemistry, 283(2), 849-854 (2007-11-16)
Hepatocytes play a crucial role in regulating lipid metabolism by exporting cholesterol and triglyceride into plasma through secretion of very low density lipoproteins (VLDL). VLDL production is also required for release of hepatitis C virus (HCV) from infected hepatocytes. Here
James W P Brown et al.
Nature communications, 10(1), 5662-5662 (2019-12-13)
Single-molecule assays have, by definition, the ultimate sensitivity and represent the next frontier in biological analysis and diagnostics. However, many of these powerful technologies require dedicated laboratories and trained personnel and have therefore remained research tools for specialists. Here, we
Kari Kusler et al.
The journal of physical chemistry. B, 120(50), 12883-12889 (2016-12-14)
Phosphatidylserine (PS) has previously been found to bind Cu2+ in a ratio of 1 Cu2+ ion per 2 PS lipids to form a complex with an apparent dissociation constant that can be as low as picomolar. While the affinity of
Erick Miranda-Laferte et al.
The Journal of biological chemistry, 289(15), 10387-10398 (2014-02-13)
The β-subunit associates with the α1 pore-forming subunit of high voltage-activated calcium channels and modulates several aspects of ion conduction. Four β-subunits are encoded by four different genes with multiple splice variants. Only two members of this family, β2a and
Stefania Zimbone et al.
International journal of molecular sciences, 21(8) (2020-04-25)
Copper plays an important role as a regulator in many pathologies involving the angiogenesis process. In cancerogenesis, tumor progression, and angiogenic diseases, copper homeostasis is altered. Although many details in the pathways involved are still unknown, some copper-specific ligands have
Anna Lívia Linard Matos et al.
Cells, 9(5) (2020-05-14)
Annexin A2 (AnxA2) is a cytosolic Ca2+ regulated membrane binding protein that can induce lipid domain formation and plays a role in exocytosis and endocytosis. To better understand the mode of annexin-membrane interaction, we analyzed membrane-bound AnxA2 assemblies by employing
Li-Ping Gao et al.
Biochimica et biophysica acta. General subjects, 1864(1), 129422-129422 (2019-09-07)
Previous studies suggested that fibrillar human IAPP (hIAPP) is more likely to deposit in β-cells, resulting in β-cell injury. However, the changes in the conformation of hIAPP in lipid environment and the mechanism involved in β-cell damage are unclear. Synthetic
Jigesh Patel et al.
Biochimica et biophysica acta. Biomembranes, 1862(2), 183140-183140 (2019-12-04)
There is an on-going debate whether anesthetic drugs, such as isoflurane, can cause meaningful structural changes in cell membranes at clinical concentrations. In this study, the effects of isoflurane on lipid membrane fluidity were investigated using fluorescence anisotropy and spectroscopy.
Cellular phosphatase activity of C1-Ten/Tensin2 is controlled by Phosphatidylinositol-3, 4, 5-triphosphate binding through the C1-Ten/Tensin2 SH2 domain
Kim E, et al.
Cellular Signalling, 51, 130-138 (2018)
Phosphatidylserine and the human brain
Glade MJ and Smith K
Nutrition, 31(6), 781-786 (2015)
Karishma Bhasne et al.
The journal of physical chemistry. B, 124(5), 708-717 (2020-01-10)
α-Synuclein is an intrinsically disordered protein that adopts an α-helical structure upon binding to the negatively charged lipid membrane. Binding-induced conformational change of α-synuclein plays a crucial role in the regulation of synaptic plasticity. In this work, we utilized the
Vanessa T R Kiraly et al.
International journal of biological macromolecules, 146, 320-331 (2020-01-04)
The Hsp70 family of heat shock proteins plays a critical function in maintaining cellular homeostasis within various subcellular compartments. The human mitochondrial Hsp70 (HSPA9) has been associated with cellular death, senescence, cancer and neurodegenerative diseases, which is the rational for
Dorotea Fracchiolla et al.
eLife, 5 (2016-11-24)
Selective autophagy is mediated by cargo receptors that link the cargo to the isolation membrane via interactions with Atg8 proteins. Atg8 proteins are localized to the membrane in an ubiquitin-like conjugation reaction, but how this conjugation is coupled to the
John M Harrington et al.
The Journal of biological chemistry, 289(36), 24811-24820 (2014-07-20)
Haptoglobin-related protein (Hpr) is a component of a minor subspecies of high density lipoproteins (HDL) that function in innate immunity. Here we show that assembly of Hpr into HDL is mediated by its retained N-terminal signal peptide, an unusual feature
Examination of the Interaction between a Membrane Active Peptide and Artificial Bilayers by Dual Polarisation Interferometry
Payne JAE, et al.
Bio-protocol, 7(1), e2087-e2087 (2017)
Josef Melcr et al.
Journal of chemical theory and computation, 16(1), 738-748 (2019-11-26)
Phosphatidylserine (PS) lipids are important signaling molecules and the most common negatively charged lipids in eukaryotic membranes. The signaling can be often regulated by calcium, but its interactions with PS headgroups are not fully understood. Classical molecular dynamics (MD) simulations
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