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Liulin Wang et al.
Langmuir : the ACS journal of surfaces and colloids, 34(36), 10764-10773 (2018-07-28)
Monoamine oxidase A and B (MAO-A and B) are mitochondrial outer membrane enzymes that are implicated in a number of human diseases, and the pharmacological inhibition of these enzymes is a promising therapeutic strategy to alleviate disease symptoms. It has
Acacia F Dishman et al.
ACS infectious diseases, 6(5), 1204-1213 (2020-04-04)
Antimicrobial peptides (AMPs) are a class of molecules which generally kill pathogens via preferential cell membrane disruption. Chemokines are a family of signaling proteins that direct immune cell migration and share a conserved α-β tertiary structure. Recently, it was found
Cameron Webb et al.
International journal of pharmaceutics, 582, 119266-119266 (2020-04-07)
Nanomedicines are well recognised for their ability to improve therapeutic outcomes. Yet, due to their complexity, nanomedicines are challenging and costly to produce using traditional manufacturing methods. For nanomedicines to be widely exploited, new manufacturing technologies must be adopted to
Caterina Masaracchia et al.
Biochimica et biophysica acta. Proteins and proteomics, 1868(1), 140298-140298 (2019-11-05)
The misfolding and aggregation of alpha-synuclein (aSyn) are thought to be central events in synucleinopathies. The physiological function of aSyn has been related to vesicle binding and trafficking, but the precise molecular mechanisms leading to aSyn pathogenicity are still obscure.
Jens H W Pahl et al.
Journal of experimental & clinical cancer research : CR, 33, 27-27 (2014-03-13)
In osteosarcoma, the presence of tumor-infiltrating macrophages positively correlates with patient survival in contrast to the negative effect of tumor-associated macrophages in patients with other tumors. Liposome-encapsulated muramyl tripeptide (L-MTP-PE) has been introduced in the treatment of osteosarcoma patients, which
Sathish Ramakrishnan et al.
Langmuir : the ACS journal of surfaces and colloids, 34(20), 5849-5859 (2018-04-26)
In vivo membrane fusion primarily occurs between highly curved vesicles and planar membranes. A better understanding of fusion entails an accurate in vitro reproduction of the process. To date, supported bilayers have been commonly used to mimic the planar membranes.
Yibo Liu et al.
Langmuir : the ACS journal of surfaces and colloids, 34(25), 7545-7553 (2018-05-29)
Natural lipid headgroups contain a few types of metal ligands, such as phosphate, amine, and serine, which interact with metal ions differently. Herein, we studied the binding between Cu2+ and liposomes with four types of headgroups: phosphocholine (PC), phosphoglycerol (PG)
Kabir H Biswas et al.
Langmuir : the ACS journal of surfaces and colloids, 34(4), 1775-1782 (2017-12-28)
The pathway of vesicle adsorption onto a solid support depends on the material composition of the underlying support, and there is significant interest in developing material-independent strategies to modulate the spectrum of vesicle-substrate interactions on a particular surface. Herein, using
Choosing the right fluorophore for single-molecule fluorescence studies in a lipid environment
Zhang Z, et al.
Biochimica et Biophysica Acta - Biomembranes, 1859(7), 1242- 1253 (2017)
Megan B Miller et al.
The Journal of biological chemistry, 290(21), 13541-13555 (2015-04-12)
Previous studies revealed an essential role for the lipid-binding Sec14 domain of kalirin (KalSec14), but its mechanism of action is not well understood. Because alternative promoter usage appends unique N-terminal peptides to the KalSec14 domain, we used biophysical, biochemical, and
Minjoung Kyoung et al.
Nature protocols, 8(1), 1-16 (2012-12-12)
This protocol describes a single vesicle-vesicle microscopy system to study Ca(2+)-triggered vesicle fusion. Donor vesicles contain reconstituted synaptobrevin and synaptotagmin-1. Acceptor vesicles contain reconstituted syntaxin and synaptosomal-associated protein 25 (SNAP-25), and they are tethered to a PEG-coated glass surface. Donor
Xi Zhang et al.
Analytical chemistry, 90(24), 14387-14394 (2018-11-15)
Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system
Philippe Calvez et al.
Journal of the American Chemical Society (2016-10-01)
Recoverin undergoes a calcium-myristoyl switch during visual phototransduction. Indeed, calcium binding by recoverin results in the extrusion of its myristoyl group, which allows its membrane binding. However, the contribution of particular lipids and of specific amino acids of recoverin in
Javier Espadas et al.
Nature communications, 10(1), 5327-5327 (2019-11-24)
The endoplasmic reticulum (ER) is a continuous cell-wide membrane network. Network formation has been associated with proteins producing membrane curvature and fusion, such as reticulons and atlastin. Regulated network fragmentation, occurring in different physiological contexts, is less understood. Here we
A single vesicle-vesicle fusion assay for in vitro studies of SNAREs and accessory proteins
Diao J, et al.
Nature Protocols, 7(5), 921-921 (2012)
Phosphatidylserine and the human brain
Glade MJ and Smith K
Nutrition, 31(6), 781-786 (2015)
Stephen MacDonald et al.
International journal of laboratory hematology (2019-11-21)
There is an unmet need to characterize the diagnosis and management of patients with an unclassified bleeding disorder (UBD). Retrospective review of registered patients with UBD at our centre. Assessment including rotational thromboelastometry (ROTEM) and thrombin generation (TG) were used.
Erdem Karatekin et al.
Nature protocols, 7(5), 903-920 (2012-04-21)
Many biological processes rely on membrane fusion, and therefore assays to study its mechanisms are necessary. Here we report an assay with sensitivity to single-vesicle, and even to single-molecule events using fluorescently labeled vesicle-associated v-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein
Tuo-Xian Tang et al.
Biochimica et biophysica acta. Biomembranes, 1862(6), 183230-183230 (2020-03-04)
Changes in membrane curvature are required to control the function of subcellular compartments; malfunctions of such processes are associated with a wide range of human diseases. Membrane remodeling often depends upon the presence of phosphoinositides, which recruit protein effectors for
Anna Sundborger et al.
Journal of cell science, 124(Pt 1), 133-143 (2010-12-22)
Clathrin-mediated vesicle recycling in synapses is maintained by a unique set of endocytic proteins and interactions. We show that endophilin localizes in the vesicle pool at rest and in spirals at the necks of clathrin-coated pits (CCPs) during activity in
Huan Bao et al.
Nature, 554(7691), 260-263 (2018-02-09)
The fusion pore is the first crucial intermediate formed during exocytosis, yet little is known about the mechanisms that determine the size and kinetic properties of these transient structures. Here, we reduced the number of available SNAREs (proteins that mediate
Jinxia Lu et al.
The Journal of biological chemistry, 295(21), 7470-7480 (2020-04-16)
Amyloid aggregation of pathological proteins is closely associated with a variety of neurodegenerative diseases, and α-synuclein (α-syn) deposition and Tau tangles are considered hallmarks of Parkinson's disease and Alzheimer's disease, respectively. Intriguingly, α-syn and Tau have been found to co-deposit
Toshihiko Oka et al.
Langmuir : the ACS journal of surfaces and colloids, 33(43), 12487-12496 (2017-10-03)
Electrostatic interactions (EIs) play important roles in the structure and stability of inverse bicontinuous cubic (QII) phases of lipid membranes. We examined the effect of pH on the phase of dioleoylphosphatidylserine (DOPS)/monoolein (MO) membranes at low ionic strengths using small-angle
Xiaoke Chi et al.
eLife, 9 (2020-01-25)
The Bcl-2 family BH3 protein Bim promotes apoptosis at mitochondria by activating the pore-forming proteins Bax and Bak and by inhibiting the anti-apoptotic proteins Bcl-XL, Bcl-2 and Mcl-1. Bim binds to these proteins via its BH3 domain and to the
Kelly Cathcart et al.
Membranes, 5(4), 664-684 (2015-11-04)
Cellular membranes have a complex phospholipid composition that varies greatly depending on the organism, cell type and function. In spite of this complexity, most structural data available for phospholipid bilayers concern model systems containing only one or two different phospholipids.
Hye-Ra Lee et al.
International journal of molecular sciences, 21(3) (2020-02-06)
Hepatitis C virus (HCV) p7 is known to be a nonselective cation channel for HCV maturation. Because the interaction of HCV proteins with host lipids in the endoplasmic reticulum membrane is crucial for the budding process, the identification of p7-lipid
Baharan Ali Doosti et al.
Langmuir : the ACS journal of surfaces and colloids, 33(41), 11010-11017 (2017-09-15)
Experimental and theoretical studies on ion-lipid interactions predict that binding of calcium ions to cell membranes leads to macroscopic mechanical effects and membrane remodeling. Herein, we provide experimental evidence that a point source of Ca2+ acting upon a negatively charged
Sathish Ramakrishnan et al.
eLife, 9 (2020-05-14)
Calcium (Ca2+)-evoked release of neurotransmitters from synaptic vesicles requires mechanisms both to prevent un-initiated fusion of vesicles (clamping) and to trigger fusion following Ca2+-influx. The principal components involved in these processes are the vesicular fusion machinery (SNARE proteins) and the
Lydie Vamparys et al.
Biophysical journal, 104(3), 585-593 (2013-02-28)
In biological membranes, changes in lipid composition or mechanical deformations produce defects in the geometrical arrangement of lipids, thus allowing the adsorption of certain peripheral proteins. Here, we perform molecular dynamics simulations on bilayers containing a cylindrical lipid (PC) and
Matteo Runfola et al.
Scientific reports, 10(1), 204-204 (2020-01-16)
The aggregation of α-synuclein (αS), a protein abundant at presynaptic terminals, is associated with a range of highly debilitating neurodegenerative conditions, including Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy (MSA). Emerging evidence indicates that the
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