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Membrane Structure and Dynamics
Cell Biology, 227-239 (2017)
Brandt Bertrand et al.
Biochimica et biophysica acta. Biomembranes, 1862(2), 183105-183105 (2019-11-05)
The aim of this study was to investigate the factors that govern the activity and selectivity of two potent antimicrobial peptides (AMPs) using lipid membrane models of bacterial, erythrocyte and fungal cells. These models were used in calcein liposome leakage
Bharat Reddy et al.
eLife, 8 (2019-12-28)
Prokaryotic mechanosensitive (MS) channels open by sensing the physical state of the membrane. As such, lipid-protein interactions represent the defining molecular process underlying mechanotransduction. Here, we describe cryo-electron microscopy (cryo-EM) structures of the E. coli small-conductance mechanosensitive channel (MscS) in
Tatiana M Domingues et al.
Langmuir : the ACS journal of surfaces and colloids, 36(19), 5145-5155 (2020-04-28)
Antimicrobial peptides are innate host defense molecules with the ability to kill pathogens. They have been widely studied for their membrane lytic activity and their potential to overcome the ever-increasing threat of antimicrobial resistance against conventional antibiotics. Here, we focus
Nabanita Das et al.
Nature protocols, 8(11), 2256-2270 (2013-10-26)
Solid-state NMR spectroscopy has been used successfully for characterizing the structure and dynamics of membrane proteins as well as their interactions with other proteins in lipid bilayers. Such an environment is often necessary for achieving native-like structures. Sample preparation is
Danubia Batista Martins et al.
Amino acids, 52(5), 725-741 (2020-05-06)
Increasing resistance in antibiotic and chemotherapeutic treatments has been pushing studies of design and evaluation of bioactive peptides. Designing relies on different approaches from minimalist sequences and endogenous peptides modifications to computational libraries. Evaluation relies on microbiological tests. Aiming a
Lydie Vamparys et al.
Biophysical journal, 104(3), 585-593 (2013-02-28)
In biological membranes, changes in lipid composition or mechanical deformations produce defects in the geometrical arrangement of lipids, thus allowing the adsorption of certain peripheral proteins. Here, we perform molecular dynamics simulations on bilayers containing a cylindrical lipid (PC) and
Ming Li et al.
Biochimica et biophysica acta. Biomembranes, 1862(9), 183315-183315 (2020-04-19)
The spontaneously formed structures of physiologically relevant lipid model membranes made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) and 1,2-hexanoyl-sn-glycero-3-phosphocholine have been evaluated in depth using small angle neutron scattering. Although a common molar ratio of long- to short- chain phospholipids (~4) as reported
Enrico Forbrig et al.
Langmuir : the ACS journal of surfaces and colloids, 34(6), 2373-2385 (2018-01-23)
Antimicrobial peptides (AMPs) are the first line of defense after contact of an infectious invader, for example, bacterium or virus, with a host and an integral part of the innate immune system of humans. Their broad spectrum of biological functions
Wenting Wang et al.
The journal of physical chemistry. B, 124(25), 5169-5176 (2020-05-27)
Membrane fusion of the viral and host cell membranes is the initial step of virus infection and is catalyzed by fusion peptides. Although the β-sheet structure of fusion peptides has been proposed to be the most important fusion-active conformation, it
Gopal Pandit et al.
Biochimica et biophysica acta. Biomembranes, 1862(4), 183177-183177 (2020-01-19)
The widespread abuse of antibiotics has led to the use of antimicrobial peptides (AMPs) as a replacement for the existing conventional therapeutic agents for combating microbial infections. The broad-spectrum activity and the resilient nature of AMPs has mainly aggrandized their
A peptide for transcellular cargo delivery: Structure-function relationship and mechanism of action.
Alexander Komin et al.
Journal of controlled release : official journal of the Controlled Release Society, 324, 633-643 (2020-06-01)
The rate of transport of small molecule drugs across biological barriers, such as the blood-brain barrier, is often a limiting factor in achieving a therapeutic dose. One proposed strategy to enhance delivery across endothelial or epithelial monolayers is conjugation to
Hannah Cetuk et al.
Langmuir : the ACS journal of surfaces and colloids, 36(18), 5065-5077 (2020-04-21)
Piscidins 1 and 3 (P1 and P3) are potent antimicrobial peptides isolated from striped bass. Their mechanism of action involves formation of amphipathic α-helices on contact with phospholipids and destabilization of the microbial cytoplasmic membrane. The peptides are active against
Philipp M G Löffler et al.
Methods in molecular biology (Clifton, N.J.), 2105, 75-96 (2020-02-24)
Peptide nucleic acids (PNAs) can be modified with aliphatic lipid chains and designed to be water soluble and able to spontaneously insert into phospholipid bilayers. Liposomes with 1.5% negatively charged POPG can be driven to fuse and mix their inner
Xinxing Zhang et al.
Journal of the American Chemical Society (2018-11-22)
Nature carefully designs the components of amphiphile-composed monolayer and bilayer membranes to deliver specific functions. The compositions of these interfacial layered structures are so delicate that minute modifications can result in huge changes in function. Great efforts have been expended
Alexander Kehl et al.
Microorganisms, 8(5) (2020-05-10)
Enterohemorrhagic Escherichia coli (EHEC) is a zoonotic pathogen responsible for life-threating diseases such as hemolytic uremic syndrome. While its major virulence factor, the Shiga toxin (Stx), is known to exert its cytotoxic effect on various endothelial and epithelial cells when
Matteo Runfola et al.
Scientific reports, 10(1), 204-204 (2020-01-16)
The aggregation of α-synuclein (αS), a protein abundant at presynaptic terminals, is associated with a range of highly debilitating neurodegenerative conditions, including Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy (MSA). Emerging evidence indicates that the
Martina Rangl et al.
eLife, 8 (2019-11-28)
CorA, a divalent-selective channel in the metal ion transport superfamily, is the major Mg2+-influx pathway in prokaryotes. CorA structures in closed (Mg2+-bound), and open (Mg2+-free) states, together with functional data showed that Mg2+-influx inhibits further Mg2+-uptake completing a regulatory feedback
Sarah M Fantin et al.
Analytical chemistry, 91(24), 15469-15476 (2019-11-20)
Membrane proteins represent most current therapeutic targets, yet remain understudied due to their insolubility in aqueous solvents and generally low yields during purification and expression. Ion mobility-mass spectrometry and collision induced unfolding experiments have recently garnered attention as methods capable
Taiji Okano et al.
ACS synthetic biology, 7(2), 739-747 (2018-02-01)
The shapes of giant unilamellar vesicles (GUVs) enclosing polymer molecules at relatively high concentration, used as a model cytoplasm, significantly differ from those containing only small molecules. Here, we investigated the effects of the molecular weights and concentrations of polymers
Takeshi Sunami et al.
Langmuir : the ACS journal of surfaces and colloids, 34(1), 88-96 (2017-12-08)
Liposome fusion is a way of supplying additional components for in-liposome biochemical reactions. Electrofusion is a method that does not require the addition of fusogens, which often alter the liposome dispersion, and is therefore useful for repetitive liposome fusion. However
Salma Al-Adwani et al.
Scientific reports, 10(1), 2376-2376 (2020-02-13)
Arginine residues of the antimicrobial peptide LL-37 can be citrullinated by peptidyl arginine deiminases, which reduce the positive charge of the peptide. Notably, citrullinated LL-37 has not yet been detected in human samples. In addition, functional and biophysical properties of
Ersoy Cholak et al.
Biochemistry, 58(50), 5052-5065 (2019-11-21)
A hallmark of Parkinson's disease is the presence of Lewy bodies consisting of lipids and proteins, mainly fibrillated α-synuclein (aSN). aSN is an intrinsically disordered protein exerting its physiological role in an ensemble of states, one of which coexists in
Sandeep Kumar et al.
The journal of physical chemistry. B, 122(26), 6763-6770 (2018-06-08)
The interaction of amphiphilic ionic liquids containing an 1-alkyl-3-methylimidazolium cation ([C12MIM]+), which shows acute cytotoxicity toward marine and bacterial life, with zwitterionic 1-palmitoyl-2-oleoyl- sn-glycero-3-phospho-choline (POPC) and anionic 1-palmitoyl-2-oleoyl- sn-glycero-3-phospho- rac-glycerol (POPG) membranes was investigated. Biophysical parameters of this interaction were
Sungsoo Lee et al.
Journal of visualized experiments : JoVE, (77), e50436-e50436 (2013-07-31)
To study the lipid-protein interaction in a reductionistic fashion, it is necessary to incorporate the membrane proteins into membranes of well-defined lipid composition. We are studying the lipid-dependent gating effects in a prototype voltage-gated potassium (Kv) channel, and have worked
Nareh Movsesian et al.
Langmuir : the ACS journal of surfaces and colloids, 34(30), 9025-9035 (2018-07-03)
In this study, we prepare giant lipid vesicles using vapor-deposited charged microporous poly(methacrylic acid- co-ethylene glycol diacrylate) polymer membranes with different morphologies and thicknesses. Our results suggest that vesicle formation is favored by thinner, more structured porous hydrogel substrates. Electrostatic
Bishoy Khairalla et al.
Bioelectrochemistry (Amsterdam, Netherlands), 132, 107443-107443 (2019-12-24)
Planar asymmetric lipid bilayers composed of phosphatidylethanolamine and phosphatidylglycerol lipids are transferred onto a gold electrode surface. Lipids containing two saturated, one monounsaturated and two monounsaturated hydrocarbon chains compose the model membranes. Results of electrochemically controlled polarization modulation infrared reflection
Hui Su et al.
ACS applied materials & interfaces, 11(47), 43799-43810 (2019-10-30)
Membrane biosensors that can rapidly sense pathogen interaction and disrupting agents are needed to identify and screen new drugs to combat antibiotic resistance. Bioelectronic devices have the capability to read out both ionic and electrical signals, but their compatibility with
Thais A Enoki et al.
Langmuir : the ACS journal of surfaces and colloids, 34(5), 2014-2025 (2017-12-29)
Considering the known different mode of action of antimicrobial peptides in zwitterionic and anionic cell membranes, the present work compares the action of the antimicrobial peptide K0-W6-Hya1 (KIFGAIWPLALGALKNLIK-NH2) with zwitterionic and negatively charged model membranes, namely, liposomes composed of phosphatidylcholine
Aishwarya Agarwal et al.
Biochimica et biophysica acta. Proteins and proteomics, 1868(2), 140324-140324 (2019-11-20)
Excitation energy migration via homo-Förster resonance energy transfer (homo-FRET) can serve as an intermolecular proximity ruler within complex biomolecular assemblies. Here we present a unique case to demonstrate that energy migration can be a novel and sensitive readout to capture
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