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显示 1-30 共 46 条结果 关于 "850356P" 范围 论文
Dene R Littler et al.
The Journal of biological chemistry, 279(10), 9298-9305 (2003-11-14)
Most proteins adopt a well defined three-dimensional structure; however, it is increasingly recognized that some proteins can exist with at least two stable conformations. Recently, a class of intracellular chloride ion channel proteins (CLICs) has been shown to exist in
Tim Diederichs et al.
Nature communications, 10(1), 5018-5018 (2019-11-07)
Nanopores are key in portable sequencing and research given their ability to transport elongated DNA or small bioactive molecules through narrow transmembrane channels. Transport of folded proteins could lead to similar scientific and technological benefits. Yet this has not been
Franziska Ramm et al.
Scientific reports, 10(1), 2887-2887 (2020-02-23)
Bacillus cereus is increasingly recognized as an opportunistic pathogen causing local and systemic infections. The causative strains typically produce three pore-forming enterotoxins. This study focusses on the tripartite non-hemolytic enterotoxin (Nhe). Until today, studies have tried to elucidate the structure
ZhangFei Su et al.
Langmuir : the ACS journal of surfaces and colloids, 36(1), 409-418 (2019-12-10)
The structure of water in the submembrane region of the bilayer of DPhPC floating (fBLM) on a monolayer of 1-thio-β-d-glucose (β-Tg)-modified gold nanoparticle film was studied by the surface-enhanced infrared absorption spectroscopy (SEIRAS). SEIRAS employs surface enhancement of the mean
Gabriel Villar et al.
Science (New York, N.Y.), 340(6128), 48-52 (2013-04-06)
Living cells communicate and cooperate to produce the emergent properties of tissues. Synthetic mimics of cells, such as liposomes, are typically incapable of cooperation and therefore cannot readily display sophisticated collective behavior. We printed tens of thousands of picoliter aqueous
Christian Bech Rosen et al.
Communications biology, 3(1), 160-160 (2020-04-05)
Protein post-translational translocation is found at the plasma membrane of prokaryotes and protein import into organellae. Translocon structures are becoming available, however the dynamics of proteins during membrane translocation remain largely obscure. Here we study, at the single-molecule level, the
Jianjun Pan et al.
The journal of physical chemistry. B, 116(51), 14829-14838 (2012-12-04)
Cholesterol and ether lipids are ubiquitous in mammalian cell membranes, and their interactions are crucial in ether lipid mediated cholesterol trafficking. We report on cholesterol's molecular interactions with ether lipids as determined using a combination of small-angle neutron and X-ray
Tomas Sabirovas et al.
Biochimica et biophysica acta. Biomembranes, 1862(6), 183232-183232 (2020-03-03)
Mixed self-assembled monolayers of octadecyltrichlorosilane (OTS) and methyltrichlorosilane (MTS) were deposited via simple silanization procedure on a mechanically polished titanium surface. The monolayers act as molecular anchors for mixed hybrid bilayer lipid membranes (mhBLM) which were accomplished via vesicle fusion.
Jin Li et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(1), 1901719-1901719 (2020-01-11)
The bottom-up construction of synthetic cells with user-defined chemical organization holds considerable promise in the creation of bioinspired materials. Complex emulsions, droplet networks, and nested vesicles all represent platforms for the engineering of segregated chemistries with controlled communication, analogous to
Stephanie Tristram-Nagle et al.
Chemistry and physics of lipids, 163(6), 630-637 (2010-05-08)
Diphytanoylphosphatidylcholine (DPhyPC) is a branched chain lipid often used for model membrane studies, including peptide/lipid interactions, ion channels and lipid rafts. This work reports results of volume measurements, water permeability measurements P(f), X-ray scattering from oriented samples, and X-ray and
Chan Cao et al.
Nature communications, 10(1), 4918-4918 (2019-10-31)
Nanopore sensing is a powerful single-molecule approach for the detection of biomolecules. Recent studies have demonstrated that aerolysin is a promising candidate to improve the accuracy of DNA sequencing and to develop novel single-molecule proteomic strategies. However, the structure-function relationship
Elio J Challita et al.
Langmuir : the ACS journal of surfaces and colloids, 34(50), 15166-15173 (2018-11-24)
Model lipid bilayer membranes provide approximations of natural cellular membranes that may be formed in the laboratory to study their mechanics and interactions with the surrounding environment. A new approach for their formation is proposed here based on the self-assembly
Alessio Sacconi et al.
Langmuir : the ACS journal of surfaces and colloids, 34(40), 12183-12190 (2018-09-16)
Solid supported membranes (SSMs) are usually formed by an hybrid octadecanethiol/phosphatidylcholine (PC) bilayer supported by a gold electrode. Recently, it was shown that phosphatidylserine (PS) in place of PC can promote a more effective accumulation of lipid vesicles on the
Unusual Constriction Zones in the Major Porins OmpU and OmpT from Vibrio cholerae
Pathania M, et al.
Structure, 26(5), 708-721 (2018)
Xiaojun Wei et al.
ACS sensors, 5(6), 1707-1716 (2020-05-15)
Nanopore technology has been employed as a powerful tool for DNA sequencing and analysis. To extend this method to peptide sequencing, a necessary step is to profile individual amino acids (AAs) through their nanopore stochastic signals, which remains a great
Zhen Fang et al.
Analytical chemistry, 92(2), 1730-1737 (2019-12-24)
Biological research and clinical testing frequently require simultaneous quantification of multiple biomarkers for accurate disease diagnosis. In particular, the application of nanopore technology to real-sample analysis is limited in terms of discriminating multiple analytes in a mixture. In this study
Wilton T Snead et al.
The Journal of cell biology, 218(2), 664-682 (2018-12-07)
Cellular membranes are continuously remodeled. The crescent-shaped bin-amphiphysin-rvs (BAR) domains remodel membranes in multiple cellular pathways. Based on studies of isolated BAR domains in vitro, the current paradigm is that BAR domain-containing proteins polymerize into cylindrical scaffolds that stabilize lipid
Physical properties of bilayer membranes formed from a synthetic saturated phospholipid in n-decane.
W R Redwood et al.
Biochimica et biophysica acta, 233(1), 1-6 (1971-03-09)
Jianfei Feng et al.
Communications biology, 3(1), 159-159 (2020-04-05)
Understanding protein folding under conditions similar to those found in vivo remains challenging. Folding occurs mainly vectorially as a polypeptide emerges from the ribosome or from a membrane translocon. Protein folding during membrane translocation is particularly difficult to study. Here
Katarina Bartoš Jansen et al.
The Journal of biological chemistry, 295(27), 9147-9156 (2020-05-14)
Colicins are Escherichia coli-specific bacteriocins that translocate across the outer bacterial membrane by a poorly understood mechanism. Group A colicins typically parasitize the proton-motive force-linked Tol system in the inner membrane via porins after first binding an outer membrane protein
Patrick Urban et al.
Langmuir : the ACS journal of surfaces and colloids, 34(44), 13368-13374 (2018-10-23)
Controlling lateral interactions between lipid molecules in a bilayer membrane to guide membrane organization and domain formation is a key factor for studying and emulating membrane functionality in synthetic biological systems. Here, we demonstrate an approach to reversibly control lipid
Zahra Aminipour et al.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 146, 133-142 (2019-11-08)
Doxorubicin is an anti-cancer drug that is important for breast cancer therapy. In this study, the effects of the membrane potential of breast cancer cells (-30 mV) and normal breast epithelial cells (-60 mV) on doxorubicin (DOX) permeability was studied. To achieve
Devika Vikraman et al.
ACS nano, 14(2), 2285-2295 (2020-01-25)
Membrane protein pores have demonstrated applications in nanobiotechnology and single-molecule chemistry for effective detection of biomolecules. Here, we define the molecular basis of carbohydrate polymers translocation through a substrate-specific bacterial nanopore, CymA, which has a 15-residue N terminus segment inside
Hadjer Ouldali et al.
Nature biotechnology, 38(2), 176-181 (2019-12-18)
Efforts to sequence single protein molecules in nanopores1-5 have been hampered by the lack of techniques with sufficient sensitivity to discern the subtle molecular differences among all twenty amino acids. Here we report ionic current detection of all twenty proteinogenic
Fujun Yao et al.
Analytical chemistry, 92(5), 3827-3833 (2020-02-13)
A crowded cellular environment is highly associated with many significant biological processes. However, the effect of molecular crowding on the translocation behavior of DNA through a pore has not been explored. Here, we use nanopore single-molecule analytical technique to quantify
Peter J Beltramo et al.
Langmuir : the ACS journal of surfaces and colloids, 34(20), 5880-5888 (2018-05-02)
Capacitance measurements provide unique insights into the thickness, compressibility, and composition of large-area membrane bilayers and are used here in addition to demonstrate the successful incorporation of model ion channels. The simultaneous ability to control the bilayer size, manipulate tension
H Lindsey et al.
Biochimica et biophysica acta, 555(1), 147-167 (1979-07-19)
We report here on a series of studies aimed at characterization of the structural and dynamical properties of the synthetic lipid diphytanoyl phosphatidylcholine, in multilamellar dispersions and vesicle suspensions. The lipid exhibits no detectable gel to liquid crystalline phase transition
Masaki Matsushita et al.
The journal of physical chemistry. B, 124(12), 2410-2416 (2020-02-08)
This paper describes a nanospace probing system constructed with a pore-forming toxin and a hairpin DNA (hpDNA) molecule. The single hpDNA molecule can be inserted and can move in the confined nanospace of the alpha-hemolysin (αHL) pore. The molecular motion
Stephen G Brohawn et al.
Nature, 516(7529), 126-130 (2014-12-05)
Activation of mechanosensitive ion channels by physical force underlies many physiological processes including the sensation of touch, hearing and pain. TRAAK (also known as KCNK4) ion channels are neuronally expressed members of the two-pore domain K(+) (K2P) channel family and
Nathan J Hardenbrook et al.
Nature communications, 11(1), 840-840 (2020-02-13)
Following assembly, the anthrax protective antigen (PA) forms an oligomeric translocon that unfolds and translocates either its lethal factor (LF) or edema factor (EF) into the host cell. Here, we report the cryo-EM structures of heptameric PA channels with partially
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