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Jayesh A Kulkarni et al.
Nanoscale, 11(45), 21733-21739 (2019-11-13)
Onpattro, the first RNAi-based therapeutic to receive FDA approval, is enabled by a lipid nanoparticle (LNP) system that facilitates siRNA delivery into the cytoplasm of target cells (hepatocytes) following intravenous (i.v.) administration. These LNP-siRNA systems consist of four lipid components
Jing Xu et al.
Molecular pharmaceutics, 10(9), 3366-3374 (2013-08-09)
Herein we report the development of a nonviral lipid-complexed PRINT (particle replication in nonwetting templates) protein particle system (LPP particle) for RNA replicon delivery with a view toward RNA replicon-based vaccination. Cylindrical bovine serum albumin (BSA) particles (diameter (d) 1
Jorge Larios et al.
The Journal of cell biology, 219(3) (2020-02-13)
The intraluminal vesicles (ILVs) of endosomes mediate the delivery of activated signaling receptors and other proteins to lysosomes for degradation, but they also modulate intercellular communication when secreted as exosomes. The formation of ILVs requires four complexes, ESCRT-0, -I, -II
Jiajie Diao et al.
Nature protocols, 7(5), 921-934 (2012-05-15)
SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins are a highly regulated class of membrane proteins that drive the efficient merger of two distinct lipid bilayers into one interconnected structure. This protocol describes our fluorescence resonance energy transfer (FRET)-based single
Amit Kumar Khan et al.
Polymers, 12(4) (2020-04-26)
In this work, we have used low-molecular-weight (PEG12-b-PCL6, PEG12-b-PCL9 or PEG16-b-PLA38; MW, 1.25-3.45 kDa) biodegradable block co-polymers to construct nano- and micron-scaled hybrid (polymer/lipid) vesicles, by solvent dispersion and electroformation methods, respectively. The hybrid vesicles exhibit physical properties (size, bilayer
Jinhong Meng et al.
Scientific reports, 10(1), 1046-1046 (2020-01-25)
P53 mutations are responsible for drug-resistance of tumour cells which impacts on the efficacy of treatment. Alternative tumour suppressor pathways need to be explored to treat p53- deficient tumours. The E3 ubiquitin ligase, ITCH, negatively regulates the tumour suppressor protein
Juliana Kovalczuk de Oliveira et al.
Materials science & engineering. C, Materials for biological applications, 110, 110720-110720 (2020-03-25)
The use of nanocarriers for drug delivery is a strategy aimed to improve therapeutic indices through changes in their pharmacokinetic and pharmacodynamic characteristics. Liposomes are well-investigated nanocarriers for drug delivery to macrophage-targeted therapy, the main hosts of intracellular pathogens of
Tjeerd Pols et al.
The FEBS journal, 288(1), 293-309 (2020-04-20)
In cells, the breakdown of arginine to ornithine and ammonium ion plus carbon dioxide is coupled to the generation of metabolic energy in the form of ATP. The arginine breakdown pathway is minimally composed of arginine deiminase, ornithine transcarbamoylase, carbamate
Katyayini Aribindi et al.
Investigative ophthalmology & visual science, 54(4), 3037-3044 (2013-04-06)
We compared phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylinositol) profiles of control and glaucomatous trabecular meshwork (TM) derived from human donors. Control TM and most primary open angle glaucoma (POAG) TM were collected from cadaver donors. A select subset of POAG
Katherine N Liu et al.
Biophysical journal, 118(10), 2426-2433 (2020-04-17)
Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which
Yu-Qing Wang et al.
International journal of pharmaceutics, 582, 119331-119331 (2020-04-15)
The major drawbacks of the cytotoxin like DM1 are the off-target effects. Here, the targeting nanovesicles were developed by synthesizing tocopherol-SS-DM1 and conjugating a pH low insertion peptide (pHLIP) to PEGylated phospholipids, in which tocopherol-SS-DM1 improves the drug loading and
Non Miyata et al.
The Journal of cell biology, 214(1), 77-88 (2016-06-30)
Phosphatidylethanolamine (PE) is an essential phospholipid for mitochondrial functions and is synthesized mainly by phosphatidylserine (PS) decarboxylase at the mitochondrial inner membrane. In Saccharomyces cerevisiae, PS is synthesized in the endoplasmic reticulum (ER), such that mitochondrial PE synthesis requires PS
Stuart A Whitehead et al.
Bioconjugate chemistry, 28(4), 923-932 (2017-03-02)
Artificial systems for controlled membrane fusion applicable for drug delivery would ideally use triggers that are orthogonal to biology. To apply the strain-promoted alkyne-azide cycloaddition (SPAAC) to drive membrane fusion, oxo-dibenzocyclooctyne (ODIBO)-lipid 1 was designed, synthesized, and studied alongside azadibenzocyclooctyne
Dariusz T Mlynarczyk et al.
Bioorganic chemistry, 96, 103634-103634 (2020-02-12)
An important subgroup within the porphyrazine (Pz) family constitutes seco-porphyrazines, in the chemical structure of which one pyrrole unit is opened in the oxidative process. So far, there are only limited data on N-seco- and C-seco-Pzs. Here, the synthesis of
Catharina Husteden et al.
ACS applied materials & interfaces, 12(8), 8963-8977 (2020-02-01)
Biomaterials, which release active compounds after implantation, are an essential tool for targeted regenerative medicine. In this study, thin multilayer films loaded with lipid/DNA complexes (lipoplexes) were designed as surface coatings for in situ transfection applicable in tissue engineering and
Benjamin J Wylie et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(1), 185-190 (2013-12-18)
It has been hypothesized that transmembrane allostery is the basis for inactivation of the potassium channel KcsA: opening the intracellular gate is spontaneously followed by ion expulsion at the extracellular selectivity filter. This suggests a corollary: following ion expulsion at
Christopher Janich et al.
Langmuir : the ACS journal of surfaces and colloids, 33(39), 10172-10183 (2017-09-06)
Four different binary lipid mixtures composed of a cationic lipid and the zwitterionic colipids DOPE or DPPC, which show different DNA transfer activities in cell culture models, were investigated at the soft air/water interface to identify transfection efficiency determining characteristics.
Takao Hanada et al.
The Journal of biological chemistry, 282(52), 37298-37302 (2007-11-08)
Autophagy is a bulk degradation process in eukaryotic cells; autophagosomes enclose cytoplasmic components for degradation in the lysosome/vacuole. Autophagosome formation requires two ubiquitin-like conjugation systems, the Atg12 and Atg8 systems, which are tightly associated with expansion of autophagosomal membrane. Previous
Ming-Tao Lee et al.
Biochemistry, 57(38), 5629-5639 (2018-08-29)
Daptomycin is a phosphatidylglycerol specific, calcium-dependent membrane-active antibiotic that has been approved for the treatment of Gram-positive infections. A recent Bacillus subtilis study found that daptomycin clustered into fluid lipid domains of bacterial membranes and the membrane binding was correlated
Manasi P Bhate et al.
Protein expression and purification, 91(2), 119-124 (2013-08-07)
We report the expression, purification, liposome reconstitution and functional validation of uniformly (13)C and (15)N isotope labeled KcsA, a bacterial potassium channel that has high homology with mammalian channels, for solid-state NMR studies. The expression and purification is optimized for
Gautam K Samdani et al.
Journal of agricultural and food chemistry, 66(15), 3939-3948 (2018-04-10)
Phospholipids have been shown to act synergistically with tocopherols and delay lipid oxidation in bulk oil. The synergistic activity between phospholipids and tocopherols is due to the ability of amino-group-containing phospholipids (e.g., phosphatidylethanolamine (PE) and phosphatidylserine (PS)) to convert oxidized
Jürgen Kreiter et al.
Biophysical journal, 117(10), 1845-1857 (2019-11-11)
Genipin, a natural compound from Gardenia jasminoides, is a well-known compound in Chinese medicine that is used for the treatment of cancer, inflammation, and diabetes. The use of genipin in classical medicine is hindered because of its unknown molecular mechanisms
Rashik Ahmed et al.
Chemical science, 10(24), 6072-6082 (2019-07-31)
Soluble amyloid beta assemblies (Aβ n
Jinju Lee et al.
Journal of controlled release : official journal of the Controlled Release Society, 321, 184-197 (2020-02-09)
Despite the promising anticancer effects of kinesin spindle protein (KSP) inhibition, functional plasticity of kinesins induced resistance against KSP inhibitors in a variety of cancers, leading to clinical failure. Additionally, paclitaxel is a widely used anticancer agent, but drug resistance
Stephen A Pearson et al.
Metallomics : integrated biometal science, 12(6), 902-915 (2020-04-28)
Iron-sulfur cluster proteins play key roles in a multitude of physiological processes; including gene expression, nitrogen and oxygen sensing, electron transfer, and DNA repair. Biosynthesis of iron-sulfur clusters occurs in mitochondria on iron-sulfur cluster scaffold proteins in the form of
Jiajun Xu et al.
Molecular pharmaceutics, 17(1), 301-315 (2019-11-26)
The controversy surrounding the use of diphtheria toxin (DT) as a therapeutic agent against tumor cells arises mainly from its unexpected harmfulness to healthy tissues. We encoded the cytotoxic fragment A of DT (DTA) as an objective gene in the
Sanjay Arora et al.
ACS chemical neuroscience, 11(11), 1620-1633 (2020-05-01)
Alzheimer's disease (AD), the most common cause of dementia, inflicts enormous suffering to patients and their family members. It is the third deadliest disease, affecting 46.8 million people worldwide. Brain-derived neurotrophic factor (BDNF) is involved in the development, maintenance, and
Parvin Zamani et al.
Life sciences, 252, 117646-117646 (2020-04-10)
Myeloid-derived suppressor cells (MDSCs) are immunosuppressive cells causing resistance to immunotherapies in cancer tumors. In the current study, various immunogenic and therapeutic features of the combination therapies with non-liposomal Doxorubicin (Dox) and the E75 immunogenic peptide (Pep), derived from the
Wei Jin et al.
Journal of the American Society for Mass Spectrometry, 31(2), 366-378 (2020-01-11)
Gemini surfactants are a class of lipid molecules that have been successfully used in vitro and in vivo as nonviral gene delivery vectors. However, the biological fate of gemini surfactants has not been well investigated. In particular, the metabolism of
Jordi H Borrell et al.
The journal of physical chemistry. B, 121(28), 6882-6889 (2017-06-22)
Because transmembrane proteins (TMPs) can be obtained with sufficient purity for X-ray diffraction studies more frequently than decades ago, their mechanisms of action may now be elucidated. One of the pending issues is the actual interplay between transmembrane proteins and
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