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L Gallego et al.
Food science and technology international = Ciencia y tecnologia de los alimentos internacional, 17(5), 429-438 (2011-09-29)
The aim of this study is to assess the use of polymeric cartridges with diode array detection to extract the main low-molecular mass phenolic compounds from red wine samples. This method expanded and improved permitted to quantify 20 compounds in
Liping Chung et al.
Breast cancer research : BCR, 16(3), R63-R63 (2014-06-18)
Serum profiling using proteomic techniques has great potential to detect biomarkers that might improve diagnosis and predict outcome for breast cancer patients (BC). This study used surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS) to identify differentially expressed proteins in
Patricia V Demkura et al.
Molecular plant, 5(3), 642-652 (2012-03-27)
Light is emerging as a central regulator of plant immune responses against herbivores and pathogens. Solar UV-B radiation plays an important role as a positive modulator of plant defense. However, since UV-B photons can interact with a wide spectrum of
Jana Škerlová et al.
The FEBS journal, 281(18), 4280-4292 (2014-05-28)
Deoxyribonucleoside regulator (DeoR) from Bacillus subtilis negatively regulates expression of enzymes involved in the catabolism of deoxyribonucleosides and deoxyribose. The DeoR protein is homologous to the sorbitol operon regulator family of metabolic regulators and comprises an N-terminal DNA-binding domain and
O Kenny et al.
Food chemistry, 161, 79-86 (2014-05-20)
Antimicrobial properties of ethanol and water extracts from eight Asteraceae species were investigated against three Gram positive (Staphylococcus aureus, MRSA and Bacillus cereus) and two Gram negative (Escherichia coli and Salmonella typhimurium) bacterial strains. Ethanol extracts from Centaurea scabiosa, Arctium
Clifton K Fagerquist et al.
Journal of the American Society for Mass Spectrometry, 23(12), 2102-2114 (2012-10-12)
We previously reported the apparent formation of matrix adducts of 3,5-dimethoxy-4-hydroxy-cinnamic acid (sinapinic acid or SA) via covalent attachment to disulfide bond-containing proteins (HdeA, Hde, and YbgS) from bacterial cell lysates ionized by matrix-assisted laser desorption/ionization (MALDI) time-of-flight-time-of-flight tandem mass
Thangarasu Silambarasan et al.
PloS one, 9(12), e115682-e115682 (2014-12-23)
Hypertensive heart disease is a constellation of abnormalities that includes cardiac fibrosis in response to elevated blood pressure, systolic and diastolic dysfunction. The present study was undertaken to examine the effect of sinapic acid on high blood pressure and cardiovascular
S.N. Singh
Climate Change and Crops, 249-249 (2009)
Andrew D Watt et al.
Methods in molecular biology (Clifton, N.J.), 752, 229-240 (2011-06-30)
Alzheimer's disease (AD) is a highly heterogeneous and progressive dementia which is characterised by a progressive decline in cognitive functioning, selective neuronal atrophy, and loss of cortical volume in areas involved in learning and memory. However, recent research has indicated
Xiao-Li Jin et al.
World journal of gastroenterology, 20(40), 14958-14964 (2014-10-31)
To develop a method to differentiate pancreatic cancer patients from healthy or benign individuals when carbohydrate antigen (CA) 19-9 is normal. Forty-one serum samples from patients with pancreatic lesions and blood samples from 20 healthy individuals were collected at the
Clara Brieke et al.
Organic & biomolecular chemistry, 13(7), 2012-2021 (2014-12-17)
Understanding the mechanisms underpinning glycopeptide antibiotic biosynthesis is key to the future ability to reinvent these compounds. For effective in vitro characterization of the crucial later steps of the biosynthesis, facile access to a wide range of substrate peptides as
Fabiola Medina et al.
Enzyme and microbial technology, 52(1), 68-76 (2012-12-04)
A Structure Activity Relationship (SAR) study for laccase mediator systems was performed in order to correctly classify different natural phenolic mediators. Decision tree (DT) classification models with a set of five quantum-chemical calculated molecular descriptors were used. These descriptors included
Hussein N Yassine et al.
PloS one, 10(1), e0115320-e0115320 (2015-01-22)
Using mass spectrometric immunoassay, the abundance of SAA truncations relative to the native variants was examined in plasma of 91 participants with type 2 diabetes and chronic kidney disease and 69 participants without diabetes. The ratio of SAA 1.1 (missing
Toshiyuki Takahashi et al.
Bioorganic & medicinal chemistry letters, 22(7), 2494-2496 (2012-03-06)
The inhibitory effects of the synthetic serotonin analogues (1-8) on COX (1 and 2) were evaluated. Two serotonin derivatives (4 and 8) showed inhibitory effect of COX (1 and 2). Especially, 4 exhibited excellent inhibitions on COX-2 with extremely high
Kimberly J Nelson et al.
Analytical biochemistry, 375(2), 187-195 (2007-12-29)
Cysteine reactivity in enzymes is imparted to a large extent by the stabilization of the deprotonated form of the reduced cysteine (i.e., the thiolate) within the active site. Although this is likely to be an important chemical attribute of many
Kyueng-Whan Min et al.
Journal of Korean medical science, 29(7), 934-940 (2014-07-22)
Direct tissue imaging mass spectrometry (IMS) by matrix-assisted laser desorption ionization and time-of-flight (MALDI-TOF) mass spectrometry has become increasingly important in biology and medicine, because this technology can detect the relative abundance and spatial distribution of interesting proteins in tissues.
Magdalena Luczak et al.
Journal of translational medicine, 13, 20-20 (2015-01-28)
Atherosclerosis is a major cause of cardiac events and mortality in patients suffering from chronic kidney disease (CKD). Moreover, the risk of cardiovascular disease (CVD) development in patients with CKD increases as kidney function declines. Although the close connection between
Anne Arnold et al.
Rapid communications in mass spectrometry : RCM, 25(19), 2844-2850 (2011-09-14)
A simple, sensitive and fast method for the determination of melamine and its derivatives in milk powder using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was developed. Neither time-consuming sample preparation, nor special target plates, or other extra equipment are
Subhro Jyoti Roy et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 50(11), 3984-3989 (2012-08-28)
In the pathology of myocardial infarction, lysosomal lipid peroxidation and resulting enzyme release play an important role. We evaluated the protective effects of sinapic acid on lysosomal dysfunction in isoproterenol induced myocardial infarcted rats. Male Wistar rats were treated with
L Pari et al.
Chemico-biological interactions, 194(1), 40-47 (2011-08-26)
Arsenic compounds are classified as toxicants and human carcinogens. Environmental exposure to arsenic imposes a big health issue worldwide. Sinapic acid is a phenylpropanoid compound and is found in various herbal materials and high-bran cereals. It has been reported that
Milica M Grozdanovic et al.
Journal of the science of food and agriculture, 94(14), 3046-3052 (2014-03-19)
Actinidin is a cysteine protease and major allergen from kiwi fruit. When purified under specific native conditions, actinidin preparations from fresh kiwi fruit contain both an active and inactive form of this enzyme. In this study, biochemical and immunological properties
Wojciech Ostrowski et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 967, 21-27 (2014-07-27)
Liquid chromatography coupled to mass spectrometry (MS) with electrospray ionization (ESI) is one of analytical techniques to obtain accurate results of low molecular weight aromatic compounds in biological samples of different origin. The interpretations of mass spectra of these aromatic
Jiyan Liu et al.
Journal of ethnopharmacology, 155(2), 1322-1331 (2014-07-30)
Chemotherapy is the mainstay of modern tuberculosis (TB) control. Traditional Chinese Medicine (TCM) can enhance the effect of anti-TB drug, promote the absorption of the foci in the lung and reduce drug toxicity. In TCM, the determination of treatment is
David M G Anderson et al.
Analytical and bioanalytical chemistry, 407(8), 2311-2320 (2015-02-11)
MALDI imaging requires careful sample preparation to obtain reliable, high-quality images of small molecules, peptides, lipids, and proteins across tissue sections. Poor crystal formation, delocalization of analytes, and inadequate tissue adherence can affect the quality, reliability, and spatial resolution of
Anand Natrajan et al.
Organic & biomolecular chemistry, 13(9), 2622-2633 (2015-01-13)
Chemiluminescent acridinium dimethylphenyl esters are highly sensitive labels that are used in automated assays for clinical diagnosis. Light emission from these labels and their conjugates is triggered by treatment with alkaline peroxide. Conjugation of acridinium ester labels is normally done
Mattia Gasperotti et al.
Talanta, 128, 221-230 (2014-07-26)
An increasing number of studies have concerned the profiling of polyphenol microbial metabolites, especially in urine or plasma, but only a few have regarded their accurate quantification. This study reports on a new ultra-performance liquid chromatography tandem mass spectrometry method
Zijun Bie et al.
Analytica chimica acta, 834, 1-8 (2014-06-15)
Boronate affinity materials have attracted increasing attentions as sample enrichment platforms for glycoproteomic analysis in recent years. However, most of the boronate affinity materials that have already employed for proteomic analysis are suffering from apparent disadvantages, such as alkaline pH
Jonathan Cartmell et al.
Carbohydrate research, 403, 123-134 (2014-08-16)
Selective strategies for the construction of novel three component glycoconjugate vaccines presenting Candida albicans cell wall glycan (β-1,2 mannoside) and polypeptide fragments on a tetanus toxoid carrier are described. The first of two conjugation strategies employed peptides bearing an N-terminal
Yin-Hung Lai et al.
The journal of physical chemistry. B, 116(32), 9635-9643 (2012-07-18)
This investigation concerns the initial chemical reactions that affect the ionization of matrixes in matrix-assisted laser desorption/ionization (MALDI). The study focuses on the relaxations of photon energy that occur on a comparable time scale to that of ionization, in which
Jose C J M D S Menezes et al.
European journal of medicinal chemistry, 46(2), 773-777 (2011-01-11)
Long chain alkyl hydroxycinnamates (8-21) were synthesized from the corresponding half esters of malonic acid (5-7) and benzaldehyde derivatives by Knoevenagel condensation. The total antioxidant capacity of these hydroxycinnamyl esters was evaluated using DPPH and ABTS assays. The observed antioxidant
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