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R Radhakrishnan et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 17(9), 1220-1223 (2013-08-10)
Blinded rechecking of auramine-stained acid-fast bacilli (AFB) sputum smears using fluorescence microscopy (FM), especially FM using light-emitting diode (LED), is not well understood. To examine the rechecking of auramine-stained sputum smears without restaining within a month using LED FM. A
Sumita Dixit et al.
Journal of AOAC International, 94(6), 1874-1881 (2012-02-11)
The present method utilizes a simple pretreatment step, cleanup on polyamide SPE cartridges, and HPLC resolution on reversed-phase C18 for the detection of the three basic nonpermitted dyes encountered in food matrixes. Polyamide cartridges were chosen because both acidic and
Akio Ohta et al.
Journal of oleo science, 57(12), 659-667 (2008-11-13)
The aggregation behaviors of three stereoisomers of tetramethylammonium N-dodecanoyl phenylalanylphenylalaninate in dilute aqueous solution were investigated. From surface tension, fluorescence intensity using probes, and heat of dilution measurements, it was suggested that the critical aggregation concentration was the same between
Nadav Amdursky et al.
The journal of physical chemistry. B, 116(45), 13389-13395 (2012-10-24)
There is an indispensable need for a fluorescence marker for the detection of amyloid fibrils, where, at present, the most used marker is thioflavin-T (ThT). Here, we present the use of auramine-O (AuO) as a possible alternative to ThT. As
The Sigma-Aldrich Handbook of Stains, Dyes and Indicators,, 103-103 (1990)
S Wilson et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 14(9), 1164-1168 (2010-09-08)
Direct sputum smear microscopy is usually less sensitive than the indirect approach using concentration by centrifugation, but laboratories often do not have access to appropriate equipment. An alternative method of sample concentration has been developed based on magnetic beads coated
Koneman's Color Atlas and Textbook of Diagnostic Microbiology (2006)
Alexander V Pichugin et al.
The American journal of pathology, 174(6), 2190-2201 (2009-05-16)
Significant host heterogeneity in susceptibility to tuberculosis exists both between and within mammalian species. Using a mouse model of infection with virulent Mycobacterium tuberculosis (Mtb), we identified the genetic locus sst1 that controls the progression of pulmonary tuberculosis in immunocompetent
Rosa M Girón et al.
Archivos de bronconeumologia, 44(12), 679-684 (2008-12-19)
To determine the prevalence of nontuberculous mycobacterial infection in patients with cystic fibrosis. We performed a prospective study in which patients with cystic fibrosis were followed for 2 years; the patients were recruited from specialized units and were all over
K E Verweij et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 14(9), 1127-1131 (2010-09-08)
Macha, Zambia. To assess the benefits of auramine-O staining fluorescence microscopy and Mycobacterial Growth Indicator Tube (MGIT) liquid culture with molecular identification in tuberculosis (TB) diagnostics. One hundred patients suspected of TB were subjected to three sputum sample examinations applying
T L Sawadogo et al.
Medecine et sante tropicales, 22(3), 302-306 (2012-11-24)
This study was conducted at the National Tuberculosis Center in Burkina Faso from October 2007 through May 2008. Our objective was to compare the diagnostic performance of three staining methods: Kinyoun, auramine O, and Ziehl-Neelsen. Ziehl-Neelsen staining served as the
Santiago Ramón-García et al.
Antimicrobial agents and chemotherapy, 56(4), 2074-2083 (2012-01-11)
Efflux pumps extrude a wide variety of chemically unrelated compounds conferring multidrug resistance and participating in numerous physiological processes. Mycobacterium tuberculosis possesses many efflux pumps, and their roles in drug resistance and physiology are actively investigated. In this work we
The epidermal cell structure of the secondary pollen presenter in Vangueria infausta (Rubiaceae: Vanguerieae) suggests a functional association with protruding onci in pollen grains.
PLoS ONE, 9, e96405-e96405 (2014)
Cindy Hendry et al.
Journal of clinical microbiology, 47(4), 1206-1208 (2009-02-27)
Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure
Analysis and testing of biological stains--the Biological Stain Commission Procedures.
D P Penney et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 77(5-6), 237-275 (2003-02-05)
Minako Kondo et al.
The journal of physical chemistry. B, 113(6), 1632-1639 (2009-01-17)
The excited-state dynamics of a reactive dye molecule, auramine O, have been studied in nanoscale water droplets stabilized by a nonionic surfactant. Spectral dynamics were measured as a function of the radius of the water nanodroplet with 50 fs time
Jessica Minion et al.
Journal of clinical microbiology, 49(5), 2024-2026 (2011-03-25)
Light-emitting diode fluorescence microscopy is being scaled up for tuberculosis control, but fading of auramine-stained slides could compromise external quality assurance. We stored auramine-stained slides and reexamined them over time. Slides stored in all environments faded quickly, with significant changes
Pavani P Nadiminti et al.
Protoplasma, 252(6), 1475-1486 (2015-02-26)
Electron microscopy techniques such as transmission electron microscopy (TEM) and scanning electron microscopy (SEM) have been invaluable tools for the study of the micromorphology of plant cuticles. However, for electron microscopy, the preparation techniques required may invariably introduce artefacts in
Taek Kwan Kwon et al.
Drug development and industrial pharmacy, 37(1), 56-61 (2010-06-10)
Monoolein (MO) cubic phase containing acidic proteinoid was prepared for a pH-dependent release. The acidic proteinoid was prepared by a thermal-condensation reaction of Asp and Leu (9.85/0.15 in molar ratio). To prepare MO cubic phase, molten MO was hydrated with
Ismael A Heisler et al.
The journal of physical chemistry. B, 113(6), 1623-1631 (2009-01-17)
The effects of confinement on the ultrafast torsional reaction of auramine O in aqueous solution are investigated through ultrafast fluorescence up-conversion with 50 fs time resolution. The aqueous solution is confined in nanoscale water droplets by an ionic surfactant. The
Garcia LS
Practical Guide to Diagnostic Parasitology (2009)
The case of the disappearing mycobacteria in Ziehl-Neelsen-stained smears.
Thomas Hänscheid et al.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 15(4), e291-e291 (2011-02-15)
Donglei Zhang et al.
Se pu = Chinese journal of chromatography, 30(8), 770-776 (2012-12-22)
A method was developed for the simultaneous determination of 10 basic dyes in meat products using ultra fast liquid chromatography-ion trap time of flight mass spectrometry (LC-IT-TOF-MS). The target analytes were separated at a flow rate of 0.2 mL/min on
Flávia T F Pacheco et al.
The Journal of parasitology, 99(6), 1002-1008 (2013-07-09)
Despite the availability of many parasitological methods for detection of Cryptosporidium and Isospora (Cystoisospora) belli in fecal samples, there are uncertainties about the accuracy of these techniques in laboratory practice. In this study, 27 formalin-fixed positive stool samples for Cryptosporidium
Saroj Hooja et al.
The Indian journal of tuberculosis, 58(2), 72-76 (2011-06-08)
In developing countries like ours with a large number of tuberculosis (TB) cases and limited resources, the diagnosis of TB relies primarily on smear microscopy for Acid Fast Bacilli (AFB) but its sensitivity is limited in paucibacillary cases. To evaluate
Verma Ajoy Kumar et al.
The Journal of communicable diseases, 40(1), 47-52 (2009-01-09)
Tuberculosis is a major threat to the public health allover world. Out of the total tuberculosis cases reported globally, more than half are reported from African continent and India. Two to three fold rises in tuberculosis cases has been reported
Leopold G Lehman et al.
The American journal of tropical medicine and hygiene, 83(4), 906-908 (2010-10-05)
Poor laboratory equipment and few human resources have made it difficult to implement microscopic diagnosis of pulmonary tuberculosis (TB) on a large scale basis worldwide. Three hundred sputum samples from patients in Cameroon were studied by using the CyScope®, a
Yuval Erez et al.
The journal of physical chemistry. A, 116(49), 12056-12064 (2012-11-28)
Both auramine-O (AuO) and thioflavin-T (ThT) behave as fluorescent molecular rotors, meaning that their (non)radiative properties are markedly affected by the intramolecular rotation of the molecule. In this article, steady-state and time-resolved fluorescence of AuO and ThT were measured in
Z Khatun et al.
Bangladesh Medical Research Council bulletin, 37(1), 7-10 (2011-06-30)
Tuberculosis remains world's leading cause of death from a single infectious agent. Fluorescence microscopy offers well-described benefits, comparing with brightfield microscopy, for the evaluation sputum smear samples for tuberculosis. We evaluated the diagnostic performance of fluorescence microscopy, using novel Light
M Habtamu et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 16(3), 402-407 (2012-05-30)
Mawenzi Regional Hospital, northern Tanzania. To determine the value of light-emitting diode (LED) microscopy in diagnosing tuberculosis (TB) on bleach-treated and direct sputum smears. Sputum samples were collected from patients suspected of pulmonary TB who presented consecutively at the laboratory
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