Complete volume to 88 µl with lysis buffer.
3. Add 88 µl of lysis buffer to the Blank control tube.
4. Add 2 µl of 25 µg/ml MGMT to the Positive control
tube and complete volume to 88
and 88> testing of un-aged and aged
samples was conducted by an accredited laboratory for
USP biological testing.
• USP : Elution Method, Agarose Overlay Method
•
up to 60 minutes.
7. Once single cells are observed, remove enough sample for counting on a hemocytometer. Then immediately add 5 mL of SCM304 + 10 µM
ROCK inhibitor to the rest.
up to 60 minutes.
7. Once single cells are observed, remove enough sample for counting on a hemocytometer. Then immediately add 5 mL of SCM304 + 10 µM
ROCK inhibitor to the rest.
detects all
isoforms of human calpain 94, including the LP82, 85,
88, and 90 forms. The antibody also detects isoforms
82, 85, 88, and 90 of rat calpain 94. The antibody
recognizes latent and active
endothelial cells,7
and CD34+ hematopoietic progenitors.8 Both TNF-α
and TNF-β bind to TNF RI. Soluble TNF-α binds with
a Kd in the range of 20-60 pM,9, 10 while TNF-β binds
with a Kd equal to 650 pM.9
endothelial cells,7
and CD34+ hematopoietic progenitors.8 Both TNF-α
and TNF-β bind to TNF RI. Soluble TNF-α binds with a
Kda in the range of 20-60 pM,9,10 while TNF-βbinds with
a Kda equal to 650 pM.9
endothelial cells,7
and CD34+ hematopoietic progenitors.8 Both TNF-α
and TNF-β bind to TNF RI. Soluble TNF-α binds with a
kDa in the range of 20-60 pM,9,10 while TNF-β binds
with a kDa equal to 650 pM
CENP-A,
conjugated to KLH, as immunogen This sequence is
highly conserved (88% identity) in bovine and dog
CENP-A shows 60% identity with histone H3. The
antibody is affinity-purified using the immunizing