90-04-0 | Sigma-Aldrich

Application Note - qPCR Assays Using the LightCycler 2.0 Instrument

Universal ProbeLibrary Probe # 106 2 x 250 reactions 04 692 250 001 Universal ProbeLibrary Set, Mouse 1 set (90 probes for 250 reactions) 04 683 641 001 MagNA Lyser Instrument (110 V)

Boc resin peptide cleavage

www.novabiochem.com 04-12-0094 04-12-0087 04-12-0015 04-12-0024 04-12-0167 04-12-0068 04-12-0012 04-12-0023 04-12-0025

Product Information Sheet - MAK513

Maltose (µM) 1 10 µL of 500 µM Std 90 µL 50 2 10 µL of 300 µM Std 90 µL 30 3 10 µL of

Product Information Sheet - MAK522

Reagent Assay Buffer 90 µL 85 µL Enzyme A 1 µL - Enzyme B 1 µL 1 µL Dye Reagent 1 µL 1 µL Transfer 90

Product Information Sheet-bgals-ro

Hydroxycorticosteroids 1 10 – 15 85 – 90 Pregnanediol 0 trace 100 Pregnanetriol trace trace 100 Estrone (O1 ) 1 – 3 10 – 15 85 – 89 Estradiols (O2 ) 1 – 3 5 – 10 90 – 95 Estriol (O3 ) 0 –

Product Information Sheet - MAK484

Standard Purified Water L-Phenyl- alanine (µM) 1 90 µL 0 µL 300 2 60 µL 30 µL 200 3 30 µL 60 µL 100 4

Product Information Sheet - MAK504

Coenzyme A (µM) 1 10 µL of 1000 µM Std 90 µL 100 2 10 µL of 600 µM Std 90 µL 60 3 10 µL of 300

ChemiScreenTM GLOW AEQUORIN CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT M1 MUSCARINIC ACETYLCHOLINE RECEPTOR

-4 0 10 20 30 40 50 60 70 80 90 100 110 120 [Carbachol] Log M ∆∆ ∆∆ re la ti ve l u m in es ce n ce u n it s (% m ax im u m ) -10 -9 -8 -7 -6 -5 -4

ChemiScreen™ FLASH AEQUORIN CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT M1 MUSCARINIC ACETYLCHOLINE RECEPTOR

10 0 10 20 30 40 50 60 70 80 90 100 110 120 [Carbachol] Log M ∆∆ ∆∆ re la ti ve l u m in es ce nc e u n it s (% m ax im u m ) -10 -9 -8 -7 -6 -5 -4

Application Note - qPCR Validation of Candidates for Modulators of the MAPkinase Signaling Pathway

Set, Human library of 90 probes 04 683 633 001 Universal ProbeLibrary Set, Mouse library of 90 probes 04 683 641 001 Universal ProbeLibrary Set, Rat library of 90 probes 04 683 650 001 Universal ProbeLibrary

Product Information Sheet-MAK465

4 5 µL 195 µL 5 5 - 200 µL 0 (Blank) 3. Mix well and transfer 10 µL of each Standard into separate wells of the plate.

Steam-in-Place Sterilization of Filtration Trains containing Milligard®PES Prefilters

0 605 10 15 20 25 30 35 40 45 50 55 65 70 75 80 85 90 95 T em p er at u re ( ºC ) Time (elapsed, 5-minute intervals) TC 01 prefilter inlet low TC

Novabiochem Innovations 02/07

; buffer B: 90/10/0.1 MeCN/water/TFA. Peptides 1-4: Gradient 0% B for 0.2 min, then 0-100% B in 18 min; flow rate: 3 ml/min. Detection: 214 nm. Peptide 5: Gradient 0% B for 0.2 min, then 0-100% B in 7

Protocol- EZMCP1-99KRM|EZMCP1-99K5PKRM|EZMCP1-99K10PKRM

Recovery % Plasma 1 0 23.0 31.25 51.0 90 62.5 69.9 75 125 128.2 84 Plasma 2 0 18.9 31.25

114792 - Test procedure MQuant Silicate / Silicat / Silicates / Silicatos

7. 75 90 7. 00 04 -X X X X X X mg/l 1.14792 Si MQuant® 3 min6 ml 20 - 4 0 °C pH 2 - 8 B

Product Information Sheet - P5380

remaining):19 • pH 6 ≈ 70% • pH 7 ≈ 80% • pH 7.5-10 ≈ 95% • pH 10.5 ≈ 90% • pH 11 ≈ 70% • pH

Novabiochem Letters 03/04

activation. Cleavage from the resin should be carried out using 90% TFA at 0 °C. Using this approach, desulfation can be reduced to less than 15% [7]. 04-12-1251 Fmoc-Tyr(SO3·NnBu4)-OH 1 g NEW 5 g NEW Derivative

Product Information Sheet - 10775207001

acids 1000 U 5000 U 04 898 133 001 04 898 141 001 rAPid Dephos and Ligation Kit Dephosphorylation of nucleic acids. 40 reactions 160 reactions 04 898 117 001 04 898 125 001 Alkaline

Human MCP-1 ELISA

Plasma 1 0 23.0 31.25 51.0 90 62.5 69.9 75 125 128.2 84 Plasma 2 0 18.9

(P6760) - Enzyme Assay

Phosphatase, Acid from potato (P6760) - Enzyme Assay P6760 Page 1 of 3 SSPNPP02 Revised: 08/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of PHOSPHATASE, ACID

(P3752) - Enzyme Assay

Phosphatase, Acid from potato (P3752) - Enzyme Assay P3752 Page 1 of 3 SSPNPP02 Revised: 08/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of PHOSPHATASE, ACID

(P1146) - Enzyme Assay

Phosphatase, Acid from potato (P1146) - Enzyme Assay P1146 Page 1 of 3 SSPNPP02 Revised: 08/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of PHOSPHATASE, ACID

MAK204 - Product Information Sheet

MAK204 Technical Bulletin Rev 04/22 3 4.

Novabiochem Innovations 05/06

-Lys(Me)2-OH · HCl 1 g 5 g 04-12-1270 Fmoc-Lys(Me3)-OH chloride 500 mg 1 g 04-12-1270 04-12-1269 04-12-1263 04-12-1264 04-12-1261

Constituents and Additives - Colorants

y (m V ) 0.0-6.00 2.550 97.550 6.01-8.00 50 50 -1.00E+04 4.00E+04 9.00E+04 1.40E+05 M S In te 0 1 2 3 4 5 Retention Time (minutes) Chromatographic Data No.

Bulletin - 704148

Analysis for 704148 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 0 2 0 4 0 6 0 8 0 1 0 0

Product Information Sheet - MAK510

1 100 μL 0 μL 35.0 2 80 μL 20 μL 28.0 3 60 μL 40 μL 21.0 4 40 μL 60 μL 14.0 5 30 μL

(P1435) - Enzyme Assay

Phosphatase, Acid from sweet potato (P1435) - Enzyme Assay P1435 Page 1 of 3 SSPNPP02 Revised: 08/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of PHOSPHATASE, ACID

(P0157) - Enzyme Assay

Phosphatase, Acid from potato (P0157) - Enzyme Assay P0157 Page 1 of 3 SSPNPP02 Revised: 08/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of PHOSPHATASE, ACID

Product Information - P1267

P1267 Page 1 of 3 SSPNPP02 Revised: 08/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of PHOSPHATASE, ACID (EC 3.1.3.2) PRINCIPLE: p-Nitrophenyl Phosphate +