then ligated with 1unit T4-DNA ligase at
pH 7.5 at 4°C. A 10 µl reaction mixture, incubated for
16 hrs. at 4°C, contained: 0.3 unit T4-DNA ligase,
66 mM Tris-HCl, 5 mM MgCl2, 1 mM dithioerythritol and
ligated with 0.1 unit T4-DNA
ligase at pH 7.5 at 4 °C. A 10 µl reaction mixture,
incubated for 16 hrs. at 4 °C, contained: 0.1 unit T4-
DNA ligase, 66 mM Tris-HCl, 5 mM MgCl2,
ligated with 0.5 units T4-DNA ligase
at pH 7.5 at 4 °C. A 10 µl reaction mixture, incubated
for 16 hrs. at 4 °C, contained: 0.5 units T4-DNA ligase,
66 mM Tris-HCl, 5 mM MgCl2, 5 mM dithioerythritol
disease in rats. J. Virol. 66:3316-3323.
Stitz, L. et al. (1992). Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna
disease in rats. J. Virology 66:3316-3323.
Bierer, B.G. et
glycerol.
Molecular mass: ∼82 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 66–90 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
(Catalog Number C7025). The proteins in
the MWGF70 Kit have a range of molecular masses
from 6.5–66 kDa.
Reagents
Approximate
Molecular Mass
• Aprotinin from bovine lung, Catalog
Number A3886
(Catalog Number C7025). The proteins in
the MWGF70 Kit have a range of molecular masses
from 6.5–66 kDa.
Reagents
Approximate
Molecular Mass
• Aprotinin from bovine lung, Catalog
Number A3886
(Catalog Number C7025). The proteins in
the MWGF70 Kit have a range of molecular masses
from 6.5–66 kDa.
Reagents
Approximate
Molecular Mass
• Aprotinin from bovine lung, Catalog
Number A3886
(Catalog Number C7025). The proteins in
the MWGF70 Kit have a range of molecular masses
from 6.5–66 kDa.
Reagents
Approximate
Molecular Mass
• Aprotinin from bovine lung, Catalog
Number A3886
Molecular Weights (kDa) of
Proteins in ColorBurst
Band Color 4 → 20% Gel 10 → 20% Gel
Tris-Glycine Tris-Tricine
Violet 220 210
Pink 100 90
Blue 60 65
Pink 45 40
Orange 30 30
Blue
nOH
Average Molecular Weight: 10,000 (8,500 - 11,500)
CAS Number: 25322-68-3
Melting Point: 61 - 66 °C
Synonyms: PEG, Carbowax, Macrogol
Polyethylene glycol (PEG) is a condensation polymer
of
Developmental Biology, 66(1), 57-71
(1978).
10. Jones, P.A. et al., Cell cycle-specific reactivation
of an inactive X-chromosome locus by 5-
azadeoxycytidine. PNAS, 79(4), 1215-1219
(1982).
mixture, incubated for
16 hours at pH 7.5 at room temperature, contained
2.0 units T4-DNA ligase, 66 mM Tris-HCl, 5 mM MgCl2,
1 mM dithioerythritol and 1 mM ATP.
The degree of ligation and subsequent
Posmantur, R., et al., J. Neurochem., 68, 2328-
2337 (1997).
8. Yagi, Y., et al., Biochem, Pharmacol., 66, 1797-
1808 (2003).
NDH/PHC 12/04
Sigma brand products are sold through
glycerol.
Molecular mass: ∼82 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 66–90 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
über die Harnstoffbildung im
Tierkörper. Hoppe-Seyler's Zeitschrift für
Physiol. Chemie. 210, 33-66.
Revised: June 2007
Sigma-Aldrich, Inc. warrants that its products conform
to the information
S62-66
(1997).
4. Parsons, R. B., et al., In vitro effect of the cysteine
metabolites homocysteic acid, homocysteine and
cysteic acid upon human neuronal cell lines.
Neurotoxicology, 19(4-5)