Acetonitrile
B: 20mM Ammonium Formate, pH3
Gradient: 0-3 min; 80% A, 3-10 min from 80 to 20% A, 10-13 min 20% A
13-15 min 20-80%A followed by 10 min re-equilibrium at initial.
Chromatographic
time;
viability > 80 %
over at least
2 passages
10 : 90 3.0 Cell density,
viability in
mid-log growth
phase
Normal cell
doubling time;
viability > 80 %
over at least
2
ammonium acetate, pH 4.5 (80:20 v/v). Total ionic strength: 20 mM
Temperature: 30 °C
Sample: Patient plasma sample treated according to sample preparation protocol.
Pressure Drop: 90 Bar (1305 psi)p ( p )
this protocol, 80 – 90% confluence of NIH3T3 in one 10-cm plate can provide approximately 80 – 90 collagen beds
on inserts. Because NIH3T3 cells grow faster than PHKs, they can be thawed 2-3 days after
doubling time; Viability >80% over at
least 2 passages
10:90 3.0 Cell density, viability in mid-log
growth phase
Normal cell doubling time; Viability >80% over at
least 2 passages
0:100 3.0 Cell
doubling time; Viability >80% over at
least 2 passages
10:90 3.0 Cell density, viability in mid-log
growth phase
Normal cell doubling time; Viability >80% over at
least 2 passages
0:100 3.0 Cell
time;
viability > 80 %
over at least
2 passages
10 : 90 3.0 Cell density,
viability in
mid-log growth
phase
Normal cell
doubling time;
viability > 80 %
over at least
2
instruments
Standard Design:
• Petri dish 90 – 100 mm, adjustable
• Horizontal and vertical flow
• Autoclavable
Easy Clean Design:
• Petri dish 90 – 100 mm, not adjustable